MD-2 Antibody

Images

 
Western Blot: MD-2 Antibody [NB100-56655] - Western Blot Image of anti-MD2. Total protein from human colon (lane 1) and human spleen (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane ...read more
Immunohistochemistry-Paraffin: MD-2 Antibody [NB100-56655] - Analysis of MD2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control antibody (top left) and MD2 antibody (bottom left, right) at ...read more
Immunohistochemistry: MD-2 Antibody [NB100-56655] - Staining of normal human brain using 100-56655 (MD-2)at 10 ug/ml.
Immunohistochemistry: MD-2 Antibody [NB100-56655] - Staining of normal human brain using control (rabbit Ig) at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MD-2 Antibody Summary

Immunogen
A full length recombinant protein was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LY96
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Publications
Read Publications using
NB100-56655 in the following applications:

  • WB
    5 publications

Reactivity Notes

This antibody is reactive against Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MD-2 Antibody

  • ESOP1
  • ESOP-1
  • LY96
  • ly-96
  • lymphocyte antigen 96
  • MD2
  • MD-2
  • myeloid differentiation protein-2
  • Protein MD-2

Background

MD2 is a secreted glycoprotein associating with the extracellular domain of Toll-like receptor 4 (TLR4) and trafficking to and from the cell surface and the Golgi apparatus. It has a signal peptide, two N terminal glycosylation sites, and seven Cys residues and consists of a heterogeneous collection of disulfide-linked oligomers. MD2 is an essential accessory glycoprotein required for LPS recognition and associates with toll-like receptor 4 on the cell surface to confer responsiveness to lipopolysaccyaride (LPS), and hence provides a link between the receptor and LPS signaling. It can also function as a soluble receptor for cells that do not otherwise express it. MD2 contains multiple trypsin cleavage sites and its expression is correlated inversely with trypsin activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MD-2 Antibody (NB100-56655)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using NB100-56655 Applications Species
Ellison MA, Gearheart CM, Porter CC, Ambruso DR. IFN-g alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils. PLoS ONE. 2017 Oct 05 [PMID: 28982143] (WB, Human) WB Human
Xiong X, Liu Y, Mei Y et al. Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling. Sci Rep. 2017 Feb 06 [PMID: 28165494] (WB, Mouse) WB Mouse
Martin I, Caban-Hernandez K, Figueroa-Santiago O, Espino AM. Fasciola hepatica Fatty Acid Binding Protein Inhibits TLR4 Activation and Suppresses the Inflammatory Cytokines Induced by Lipopolysaccharide In Vitro and In Vivo J. Immunol. 2015 Mar 16 [PMID: 25780044] (WB, Human) WB Human
Yang H, Wang H, Ju Z et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling J. Exp. Med. 2015 Jan 12 [PMID: 25559892] (WB, Human)

Details:
MD-2 antibody used for WB analysis on mixture of CBP-tagged HMGB1 or CBP alone with supernatant of yeast Sf9 cells expressing human MD-2 immunoprecipitated with calmodulin beads (Figure 1C). Antibody also used for WB on lysate of RAW 264.7 cells that were subjected or not to the knockdown of MD-2 / with siRNA-MD2 (Figure 2A).
WB Human
Kunda PE, Cavicchia JC, Acosta CG. Lipopolysaccharides and trophic factors regulate the LPS receptor complex in nodose and trigeminal neurons. Neuroscience. 2014 Sep 11 [PMID: 25218806] (WB, Mouse)

Details:
MD2 antibody used for WB on lysates of neurons from trigeminal and nodose ganglia of CD-1 mice /postnatal day 5. Spleen lysate was used as positive control and the WB Images are shown in Fig. 1A.
WB Mouse

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Bioinformatics

Gene Symbol LY96
Entrez
Uniprot