MD-1/LY86 Antibody

Western Blot: MD-1/LY86 Antibody [NB100-56700] - Analysis of MD-1 in Ramos lysate using MD-1 antibody at 2 ug/ml.

Immunohistochemistry-Paraffin: MD-1/LY86 Antibody [NB100-56700] - Analysis of human spleen using MD-1 antibody at 10 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MD-1/LY86 Antibody Summary

• Immunogen: This antibody was developed against a KLH-conjugated synthetic peptide corresponding to amino acids 112-125 of human MD-1.
• Specificity: In Ramos, a 25 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LY86
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/ml

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for MD-1/LY86 Antibody

• LY86
• Ly-86
• lymphocyte antigen 86
• MD1
• MD-1
• MMD-1
• Protein MD-1
• RP105-associated

Background

Mammalian MD-1 is a homolog of Chicken MD-1, which was previously isolated as a v-myb-regulated gene, since its transcription increases rapidly after v-myb induction. MD-1 is associated at the cell surface with RP105. RP105 is a leucine-rich repaeat (LRR) molecule that is expressed on B-lymphocytes. It was first identified by a mAb that protects spleen B cells from irradiation-induced apoptosis (2). LRR proteins, such as Toll receptors, have a role in innate immunity. MD-1 does not have any homology to other molecules. MD-1 when expressed alone behaves as a secretory protein. Expression of MD-1 in a cell increases the expression of RP105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MD-1/LY86 Antibody (NB100-56700)(3)

Publications using NB100-56700

• Kunda PE, Cavicchia JC, Acosta CG. Lipopolysaccharides and trophic factors regulate the LPS receptor complex in nodose and trigeminal neurons. Neuroscience. 2014-09-11 [PMID: 25218806] (WB, Mouse)
  
  Details:
  MD1/LY86 antibody used for WB on lysates of neurons from trigeminal and nodose ganglia of CD-1 mice /postnatal day 5. Spleen lysate was used as positive control and the WB Images are shown in Fig. 1A.
• Malek RL, Wang HY, Kwitek AE et al. Physiogenomic resources for rat models of heart, lung and blood disorders. Nat Genet. 2006-02-01 [PMID: 16415889]
• Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677]
  
  Details:
  IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).

Bioinformatics

• Gene Symbol: LY86
• Entrez:
  • 9450(Human)
  • 17084(Mouse)
• Uniprot:
  • O95711()