DNMT3A Antibody (64B1446)

Images

 
Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes. Whole mount ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Biological Strategies: Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-WhMt
Clone
64B1446
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

DNMT3A Antibody (64B1446) Summary

Immunogen
This DNMT3A Antibody (64B1446) was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • CyTOF-ready
  • Immunohistochemistry Whole-Mount
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 24623306). Use in Immunohistochemistry reported in multiple pieces of scientific literature: Immunohistochemistry Whole Mount (PMID: 26507142), Immunohistochemistry Frozen (PMID: 22134929), and Immunohistochemistry Paraffin (PMID: 22134929).
Control
DNMT3A Overexpression Lysate
DNMT3A Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB120-13888 in the following applications:

Publications
Read Publications using
NB120-13888 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for DNMT3A Antibody (64B1446)

  • 64B1446 clone
  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA Methyltransferase 3 Alpha
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA
  • TBRS

Background

Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.

References

1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L.,... Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G.,... Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D.,... Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for DNMT3A Antibody (NB120-13888)(98)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, KO, WB.


Filter By Application
ChIP
(7)
ICC/IF
(12)
IHC
(2)
IHC-Fr
(1)
IHC-P
(5)
IHC-WhMt
(3)
KO
(1)
WB
(37)
All Applications
Filter By Species
Human
(22)
Mouse
(35)
All Species
Showing Publications 1 - 10 of 98. Show All 98 Publications.
Publications using NB120-13888 Applications Species
Jia Y, Xie H, Zhang J, Ying H Induction of TGF-beta receptor I expression in a DNA methylation-independent manner mediated by DNMT3A downregulation is involved in early-onset severe preeclampsia FASEB J. Aug 14 2020 [PMID: 32794622] (WB) WB
Yagi M, Kabata M, Tanaka A et al. Identification of distinct loci for de novo DNA methylation by DNMT3A and DNMT3B during mammalian development Nat Commun Jun 24 2020 [PMID: 32581223] (WB, KO, Mouse) WB, KO Mouse
Ginno PA, Gaidatzis D, Feldmann A et al. A genome-scale map of DNA methylation turnover identifies site-specific dependencies of DNMT and TET activity Nat Commun May 29 2020 [PMID: 32471981] (WB, Mouse) WB Mouse
Ravichandran M, Lei R, Tang Q et al. Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells Cell Rep Aug 20 2019 [PMID: 31433977] (WB, Mouse) WB Mouse
Pang Y, Liu J, Li X et al. MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer.. J. Cell. Mol. Med. Oct 16 2018 [PMID: 30324719] (ICC/IF, Human) ICC/IF Human
Richard Albert J Application of an allele-specific pipeline to study DNA methylation inheritance and dynamics in the early embryo Thesis Jan 1 2020 (ICC/IF, Mouse) ICC/IF Mouse
McAninch D, Makela JA, La HM et al. SOX3 promotes generation of committed spermatogonia in postnatal mouse testes Sci Rep Apr 21 2020 [PMID: 32317665] (IHC-WhMt, Mouse) IHC-WhMt Mouse
Xia Limin, Huang Wenjie, Bellani Marina et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell 2017 Sep 06 [PMID: 28486105] (WB, ChIP, ICC/IF, Human) WB, ChIP, ICC/IF Human
Espinosa-Diez C, Wilson R, Mukherjee R et al. DNA damage dependent hypomethylation regulates the pro-angiogenic LncRNA MEG9 bioRxiv Oct 14 2018 (WB, Human) WB Human
Magno Guimaraes D, deLucas da Silva Almeida F, Moraes Castilho R DNA methyltransferase expression is associated with cell proliferation in salivary mucoepidermoid carcinoma J. Oral Pathol. Med. Aug 2 2020 [PMID: 32740989]
Show All 98 Publications.

Reviews for DNMT3A Antibody (NB120-13888) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Human , Other.

Reviews using NB120-13888:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Human
(1)
Other
(1)
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Images Ratings Applications Species Date Details
Western Blot DNMT3A NB120-13888
Enlarge
5
reviewed by:
Anonymous
WB Human 01/30/2019
View

Summary

ApplicationWestern Blot
Sample Testedcell
SpeciesHuman
LotD-5
Western Blot DNMT3A NB120-13888
Enlarge
4
reviewed by:
Anonymous
WB Human 01/23/2017
View

Summary

ApplicationWestern Blot
Sample Testedrecombinant purified protein
Species Human
LotAB1106138-C3

Comments

Comments1ug of recombinant DNMT3A protein. 4-12% Bis tris gel
Western Blot DNMT3A NB120-13888
Enlarge
4
reviewed by:
Nathan Burns
WB Other 08/02/2016
View

Summary

ApplicationWestern Blot
SpeciesOther
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB120-13888). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any information about the epitope of your antibody, so I could check the chances for the antibody working in the honey bee?
    • This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. We haven't mapped the binding binding epitope although Chen et (2002) mapped it to a range between 705-908 amino acids: I am not sure what technique you are planning with the antibody, but if you are doing a western blot, we do have a western blot postive control ready to use lysate NBP2-25063 and I would l highly suggest using that.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-13888AF350
Alexa Fluor 405 NB120-13888AF405
Alexa Fluor 488 NB120-13888AF488
Alexa Fluor 532 NB120-13888AF532
Alexa Fluor 594 NB120-13888AF594
Alexa Fluor 647 NB120-13888AF647
Alexa Fluor 700 NB120-13888AF700
Alexa Fluor 750 NB120-13888AF750
Allophycocyanin NB120-13888APC
DyLight 350 NB120-13888UV
DyLight 405 NB120-13888V
DyLight 488 NB120-13888G
DyLight 550 NB120-13888R
DyLight 594 NB120-13888DL594
DyLight 650 NB120-13888C
DyLight 680 NB120-13888FR
DyLight 755 NB120-13888IR
FITC NB120-13888F
HRP NB120-13888H
Janelia Fluor 549 NB120-13888JF549
Janelia Fluor 646 NB120-13888JF646
PE NB120-13888PE

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Blogs on DNMT3A.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
01/30/2019
Application: WB
Species: Human

Anonymous
01/23/2017
Application: WB
Species: Human

Nathan Burns
08/02/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol DNMT3A