DNMT3A Antibody (64B1446) - BSA Free


Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes. Whole mount ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Biological Strategies: Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-WhMt
BSA Free
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

DNMT3A Antibody (64B1446) - BSA Free Summary

This DNMT3A Antibody (64B1446) was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation
  • CyTOF-ready
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 24623306). Use in Immunohistochemistry reported in multiple pieces of scientific literature: Immunohistochemistry Whole Mount (PMID: 26507142), Immunohistochemistry Frozen (PMID: 22134929), and Immunohistochemistry Paraffin (PMID: 22134929).
DNMT3A Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
NB120-13888 in the following applications:

Read Publications using
NB120-13888 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34874218) .

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for DNMT3A Antibody (64B1446) - BSA Free

  • 64B1446 clone
  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA Methyltransferase 3 Alpha
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC
  • M.HsaIIIA
  • TBRS


Methylation of cytosine residues is essential for mammalian development by regulating gene expression, gene silencing and genomic imprinting (1). This process is controlled by the enzyme DNA (cytosine-5)-methyltransferase 3A (DNMt3a), which is encoded by the DNMT3A gene and has a predicted molecular weight of 102 kDa. DNA methyltransferases including DNMt3a are involved in de novo cytosine methylation and maintenance of embryonic and somatic cells through the transfer of methyl groups to specific CpG structures in DNA (2). Somatic mutations in DNMT3A are responsible for cytogenetically normal acute myeloid leukemia (CN-AML). Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA are linked with cancer (3,4). Mutations in the DNMT3A gene have also been found to cause DNMT3A overgrowth syndrome and myelodysplastic syndromes.


1. Thakur, A., Mackin, S. J., Irwin, R. E., O'Neill, K. M., Pollin, G., & Walsh, C. (2016). Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin, 9, 53. doi:10.1186/s13072-016-0104-2

2. Ravichandran, M., Lei, R., Tang, Q., Zhao, Y., Lee, J., Ma, L., . . . Dawlaty, M. M. (2019). Rinf Regulates Pluripotency Network Genes and Tet Enzymes in Embryonic Stem Cells. Cell Rep, 28(8), 1993-2003.e1995. doi:10.1016/j.celrep.2019.07.080

3. Pang, Y., Liu, J., Li, X., Xiao, G., Wang, H., Yang, G., . . . Ren, H. (2018). MYC and DNMT3A-mediated DNA methylation represses microRNA-200b in triple negative breast cancer. J Cell Mol Med, 22(12), 6262-6274. doi:10.1111/jcmm.13916

4. Xia, L., Huang, W., Bellani, M., Seidman, M. M., Wu, K., Fan, D., . . . Baylin, S. B. (2017). CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell, 31(5), 653-668.e657. doi:10.1016/j.ccell.2017.04.005


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB120-13888)(111)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, KO, WB.

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Showing Publications 1 - 10 of 111. Show All 111 Publications.
Publications using NB120-13888 Applications Species
Lu F, Yi Y, Vladimirova O et al. Epigenetic Landscape of HIV Infection in Primary Human Macrophage bioRxiv Jan 27 2022 [PMID: 35319230]
Liu X, Cui H The palliative effects of folic acid on retinal microvessels in diabetic retinopathy via regulating the metabolism of DNA methylation and hydroxymethylation Bioengineered Dec 1 2021 [PMID: 34874218] (ChIP, Rat) ChIP Rat
Aronson BE, Scourzic L, Shah V Et al. A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus Developmental cell Oct 26 2021 [PMID: 34710357]
Zhao J, Lin H, Huang K. Mesenchymal Stem Cell-derived Extracellular Vesicles Transmitting MicroRNA-34a-5p Suppress Tumorigenesis of Colorectal Cancer Through c-MYC/DNMT3a/PTEN Axis Molecular neurobiology Oct 8 2021 [PMID: 34623601] (WB, Human) WB Human
da C Fernandes CJ, da Silva RA, Fretes Wood P Et al. The molecular pathway triggered by zirconia in endothelial cells involves epigenetic control Tissue & cell Aug 18 2021 [PMID: 34425516] (WB, Human) WB Human
Kim KP, Li C, Bunina D et al. Donor cell memory confers a metastable state of directly converted cells Cell stem cell Apr 7 2021 [PMID: 33848472]
Huang Z, Yu J, Cui W, et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins Science Advances Jan 1 2021 [PMID: 33523915] (WB, Mouse) WB Mouse
Broche J, Kungulovski G, Bashtrykov P et al. Genome-wide investigation of the dynamic changes of epigenome modifications after global DNA methylation editing Nucleic acids research Dec 9 2020 [PMID: 33300025] (WB) WB
Cardenas H, Fang F, Jiang G et al. Methylomic Signatures of High Grade Serous Ovarian Cancer Epigenetics Dec 8 2020 [PMID: 33289590]
Jia Y, Xie H, Zhang J, Ying H Induction of TGF-beta receptor I expression in a DNA methylation-independent manner mediated by DNMT3A downregulation is involved in early-onset severe preeclampsia FASEB J. Aug 14 2020 [PMID: 32794622] (WB) WB
Show All 111 Publications.

Reviews for DNMT3A Antibody (NB120-13888) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using NB120-13888:
Filter by Applications
Western Blot
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Filter by Species
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Images Ratings Applications Species Date Details
Western Blot DNMT3A NB120-13888
reviewed by:
Yichi Xu
Western Blot Human 01/30/2019


ApplicationWestern Blot
Sample Testedcell
Western Blot DNMT3A NB120-13888
reviewed by:
Jennifer Fox
Western Blot Human 01/23/2017


ApplicationWestern Blot
Sample Testedrecombinant purified protein


Comments1ug of recombinant DNMT3A protein. 4-12% Bis tris gel
Western Blot DNMT3A NB120-13888
reviewed by:
Verified Customer
Western Blot Other 08/02/2016


ApplicationWestern Blot

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB120-13888). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any information about the epitope of your antibody, so I could check the chances for the antibody working in the honey bee?
    • This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. We haven't mapped the binding binding epitope although Chen et (2002) mapped it to a range between 705-908 amino acids: I am not sure what technique you are planning with the antibody, but if you are doing a western blot, we do have a western blot postive control ready to use lysate NBP2-25063 and I would l highly suggest using that.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on DNMT3A.

Epigenetics of Depression: How Can Psychological Stress Alter Your DNA?
By Emily Cartwright, PhDHow Can Psychological Stress Alter Your DNA? Traumatic events, work demands, relationship conflicts, and health problems are all examples of psychological stressors that can result in phy...  Read full blog post.

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation
By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews


Yichi Xu
Application: Western Blot
Species: Human

Jennifer Fox
Application: Western Blot
Species: Human

Verified Customer
Application: Western Blot
Species: Other


Gene Symbol DNMT3A