DNMT3A Antibody (64B1446)

Images

 
Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with ...read more
Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - Formalin-fixed, paraffin-embedded human liver stained with Dnmt3a antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by ...read more
Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred ...read more
Immunocytochemistry/ Immunofluorescence: DNMT3A Antibody (64B1446) [NB120-13888] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: DNMT3A Antibody (64B1446) [NB120-13888] - IHC-P detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC-P, IHC-WhMt
Clone
64B1446
Clonality
Monoclonal
Host
Mouse
Concentration
1.0 mg/ml

DNMT3A Antibody (64B1446) Summary

Immunogen
This DNMT3A antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT3A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunohistochemistry Whole-Mount
Application Notes
Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306). Use in Immunohistochemistry whole mount reported in scientific literature (PMID 26507142).
Positive Control
DNMT3A Lysate (NBL1-09970)
Reviewed Applications
Read 2 Reviews rated 4
using
NB120-13888 in the following applications:

Publications
Read Publications using
NB120-13888 in the following applications:

Alternate Names for DNMT3A Antibody (64B1446)

  • 64B1446 clone
  • DNA (cytosine-5-)-methyltransferase 3 alpha
  • DNA (cytosine-5)-methyltransferase 3A
  • DNA cytosine methyltransferase 3A2
  • DNA methyltransferase HsaIIIA
  • DNA MTase HsaIIIA
  • DNMT3A
  • DNMT3A2
  • EC 2.1.1.37
  • M.HsaIIIA

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNMT3A Antibody (NB120-13888)(79)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt, WB.


Filter By Application
ChIP
(5)
ICC/IF
(7)
IHC
(2)
IHC-Fr
(1)
IHC-P
(5)
IHC-WhMt
(2)
WB
(27)
All Applications
Filter By Species
Human
(16)
Mouse
(24)
All Species
Showing Publications 1 - 10 of 79. Show All 79 Publications.
Publications using NB120-13888 Applications Species
Thakur A, Mackin SJ, Irwin RE et al. Widespread recovery of methylation at gametic imprints in hypomethylated mouse stem cells following rescue with DNMT3A2. Epigenetics Chromatin. Nov 29 2016 12:00AM [PMID:27895716] (WB, Mouse) WB Mouse
Hoang TV, Horowitz ER, Chaffee BR et al. Lens development requires DNMT1 but takes place normally in the absence of both DNMT3A and DNMT3B activity. Epigenetics. Nov 8 2016 12:00AM [PMID:27824296] (Mouse) Mouse
Daniel FI, Alves SR, Vieira DS et al. Immunohistochemical expression of DNA methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas.

Details:
DNMT3A antibody (clone 64B1446; Imgenex IMG-268A) was used at 1:700 dilution for IHC-P analysis of tissues from human subjects with actinic cheilitis and lip squamous cell carcinoma (LSCC), and non-neoplastic tissue (Figure 1). J. Oral Pathol. Med. May 9 2016 12:00AM [PMID:27159259] (IHC-P, Human)
IHC-P Human
Xu X, Tan X, Hulshoff MS et al. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016 Apr 01 [PMID:27012941] (WB, Human) WB Human
Hong B, Su Z, Zhang C et al. Reserpine Inhibit the JB6 P+ Cell Transformation Through Epigenetic Reactivation of Nrf2-Mediated Anti-oxidative Stress Pathway. AAPS J. 2016 Mar 17 [PMID:26988984] (WB, Mouse) WB Mouse
Dhawan S, Dirice E, Kulkarni RN, Bhushan A. Inhibition of TGF-beta signaling promotes human pancreatic beta cell replication. Diabetes. 2016 Mar 02 [PMID:26936960] (WB, Human) WB Human
Amouroux R, Nashun B, Shirane K et al. De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat. Cell Biol. 2016 Feb 01 [PMID:26751286] (ICC/IF, Mouse) ICC/IF Mouse
Li Y, Seah MK, O'Neill C. Mapping global changes in nuclear cytosine base modifications in the early mouse embryo.

Details:
DNMT3A Antibody (clone 64B1446; Imgenex IMG-268A) was used for immunofluorescence staining of the murine whole embryos which were fixed at in 4% formaldehyde for 30?minutes at room temperature and permeabilized in 0.5% Tween20 & Triton X100. The primary antibody was used at 1:1000 dilution (see full text for detailed protocol). Reproduction. 2016 Feb 01 [PMID:26660107] (IHC-WhMt, Mouse)
IHC-WhMt Mouse
Tsukada Y, Akiyama T, Nakayama KI. Maternal TET3 is dispensable for embryonic development but is required for neonatal growth.

Details:
DNMT3a antibody was used for ICC/IF and IHC-whole mount staining of fully grown oocytes at the germinal vesicle stage and zygotes produced by in vitro fertilization in experiments involving Tet3 conditional knockout mice. The IHC protocol involved 20?minutes of fixation at RT in 4% PFA-PBS with 0.2% Triton X-100, 1 hour blocking with 1% BSA -0.05% Tween 20, ON incubation with DNMT3a antibody at 1:1000 dilution, detection using AF568-conjugated secondary (see full text for detailed protocol). Sci Rep. 2015 Oct 28 [PMID:26507142] (IHC-WhMt, ICC/IF, Mouse)
IHC-WhMt, ICC/IF Mouse
Dhawan S, Tschen SI, Zeng C et al. DNA methylation directs functional maturation of pancreatic B cells J. Clin. Invest. 2015 Jul 01 [PMID:26098213] (IHC-P, Mouse) IHC-P Mouse
Show All 79 Publications.

Reviews for DNMT3A Antibody (NB120-13888) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human , Other.
We have 2 review tested in 1 application: WB.

Reviews using NB120-13888:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DNMT3A NB120-13888
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reviewed by:
Anonymous
WB Human 01/23/2017
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Summary

ApplicationWestern Blot
Sample Testedrecombinant purified protein
Species Human
LotAB1106138-C3

Comments

Comments1ug of recombinant DNMT3A protein. 4-12% Bis tris gel
Western Blot DNMT3A NB120-13888
Enlarge
reviewed by:
Nathan Burns
WB Other 08/02/2016
View

Summary

ApplicationWestern Blot
SpeciesOther
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for DNMT3A Antibody (NB120-13888). (Showing 1 - 1 of 1 FAQs).

  1. Do you have any information about the epitope of your antibody, so I could check the chances for the antibody working in the honey bee?
    • This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. We haven't mapped the binding binding epitope although Chen et (2002) mapped it to a range between 705-908 amino acids: I am not sure what technique you are planning with the antibody, but if you are doing a western blot, we do have a western blot postive control ready to use lysate NBP2-25063 and I would l highly suggest using that.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-13888AF405
Alexa Fluor 488 Labeled NB120-13888AF488
Alexa Fluor 647 Labeled NB120-13888AF647
Alexa Fluor 700 Labeled NB120-13888AF700
Allophycocyanin Labeled NB120-13888APC
DyLight 350 Labeled NB120-13888UV
DyLight 405 Labeled NB120-13888V
DyLight 488 Labeled NB120-13888G
DyLight 550 Labeled NB120-13888R
DyLight 650 Labeled NB120-13888C
DyLight 680 Labeled NB120-13888FR
DyLight 755 Labeled NB120-13888IR
HRP Labeled NB120-13888H
PE Labeled NB120-13888PE
PerCP Labeled NB120-13888PCP

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Blogs on DNMT3A.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Go Ahead! Make My DNA
DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

Contact Information

Product PDFs

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
01/23/2017
Application: WB
Species: Human

Nathan Burns
08/02/2016
Application: WB
Species: Other

Bioinformatics

Gene Symbol DNMT3A
Entrez
Uniprot

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