MeCP2 Antibody

Images

 
Western Blot: MeCP2 Antibody [NB100-56326] - analysis of MeCP2 in HeLa cell lysate using this antibody.
Immunocytochemistry/ Immunofluorescence: MeCP2 Antibody [NB100-56326] - MeCP2 antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against ...read more
Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - IHC analysis of a formalin fixed paraffin embedded section of mouse liver tissue using MeCP2 antibody at 1:500 dilution. The antibody generated a very ...read more
Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using MeCP2 antibody at 1:500 dilution. The antibody generated a very ...read more
Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - IHC analysis of a formalin fixed paraffin embedded section of mouse liver tissue using MeCP2 antibody at 1:500 dilution. The antibody generated a very ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MeCP2 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 11-25 and 181-195 of human MeCP2.
Localization
Nucleus
Marker
Nucleus Marker
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MECP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
In HeLa, a 53 kDa band is observed.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-56326.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MeCP2 Antibody

  • AUTSX3
  • DKFZp686A24160
  • MeCp-2 protein
  • mental retardation, X-linked 79
  • methyl CpG binding protein 2 (Rett syndrome)
  • X-linked 16

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MeCP2 Antibody (NB100-56326)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MeCP2 Antibody (NB100-56326) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MeCP2 Antibody (NB100-56326)

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Research Areas for MeCP2 Antibody (NB100-56326)

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Blogs on MeCP2.

New MECP2 Study Suggests Important Link to GABA
MECP2 antibodies are used in DNA methylation studies as well as research into Rett syndrome, a progressive neurological disorder caused by a mutation in the MECP2 gene. Now, a new study has been published by the laboratory where the Rett Syndrome gene...  Read full blog post.

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Bioinformatics

Gene Symbol MECP2
Entrez
Uniprot