CYP51A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex, colon, liver and testis using Anti-CYP51A1 antibody NBP2-48733 (A) shows similar protein distribution across tissues to independent antibody NBP2-48793 (B).

Western Blot: CYP51A1 Antibody [NBP2-48733] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: CYP51A1 Antibody [NBP2-48733] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis using Anti-CYP51A1 antibody NBP2-48733.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human liver.

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human colon.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CYP51A1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: RSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQH
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CYP51A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CYP51A1 Recombinant Protein Antigen (NBP2-48733PEP)

Reactivity Notes

Mouse (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CYP51A1 Antibody - BSA Free

• CP51
• CYP51
• CYP51A1
• CYP51Cytochrome P450LI
• CYPL1
• CYPLI
• Cytochrome P450 51A1
• cytochrome P450, family 51, subfamily A, polypeptide 1
• Cytochrome P45014DM
• Cytochrome P450-14DM
• Cytochrome P450LI
• EC 1.14.13.70
• EC 1.14.14.154
• lanosterol 14-alpha demethylase
• lanosterol 14-alpha-demethylase
• LDM
• LDMCytochrome P45014DM
• P450-14DM
• P450L1,51 (lanosterol 14-alpha-demethylase)
• Sterol 14-alpha demethylase

Background

CYP51A1, also known as Lanosterol 14-alpha demethylase, consists of a 56.8 kDa and a 46.3 kDa isoform and is involved in transforming lanosterol by removing the 14alpha-methyl group. Current research is being conducted with several diseases including tuberculosis, antley-bixler syndrome, colorectal cancer, leukemia, immunodeficiency, prostatitis, cholesterol, and chagas disease. This protein has been linked to the cholesterol and steroid biosynthesis, metabolism, and signaling pathways, where it interacts with FA2H, MSMO1, SCD, ZMPSTE24, and FDFT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYP51A1