Genetic Strategies: Knockdown Validated: MBD1 Antibody (100B272.1) [NB100-56537] - blot shows lysates of HEK293T human embryonic kidney parental cell line and MBD1 knockout (KO) HEK293T cell line. PVDF membrane ...read more
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - NIH3T3 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of MBD1 (100B272.1) antibody. The staining was developed using HRP labeled ...read more
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
Immunocytochemistry/ Immunofluorescence: MBD1 Antibody (100B272.1) [NB100-56537] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|
There are many isoforms of human MBD1 ranging from 503 (~55 kDa) to 655 (~71 kDa) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 25798578). Use in Immunoprecipitation reported in scientific literature (PMID: 15327775). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24464130). Use in ICC/IF was reported in scientific literature (PMID: 24464130).
Mouse reactivity reported in scientific literature (PMID: 25798578)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
Alternate Names for MBD1 Antibody (100B272.1)
CXXC-type zinc finger protein 3
methyl-CpG binding domain protein 1 isoform PCM1
methyl-CpG binding domain protein 1
methyl-CpG-binding domain protein 1
Methyl-CpG-binding protein MBD1
Protein containing methyl-CpG-binding domain 1
the regulator of fibroblast growth factor 2 (FGF-2) transcription
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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