MBD1 Antibody (100B272.1)

Images

 
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - analysis of Jurkat cell lysate using anti-MBD1 antibody. Image from verified customer review.
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis of MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).
Western Blot: MBD1 Antibody (100B272.1) [NB100-56537] - Analysis using Azide and BSA Free version of NB100-56537. MBD1 in HeLa lysate using MBD1 antibody at 2 ug/ml (lane 1) and 1 ug/ml (lane 2).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IP
Clone
100B272.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

MBD1 Antibody (100B272.1) Summary

Immunogen
This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|
Specificity
There are many isoforms of human MBD1 ranging from 503 (~ 55kD) to 655 (~ 71kD) amino acids in length. Researchers are encouraged to use the provided immunogen sequence to determine if this antibody is suitable for testing a specific isoform of interest.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoprecipitation
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 25798578). Use in Immunoprecipitation reported in scientific literature (PMID 15327775)
Positive Control
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 3
using
NB100-56537 in the following applications:

Publications
Read Publications using
NB100-56537 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    2 publications
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25798578)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MBD1 Antibody (100B272.1)

  • CXXC-type zinc finger protein 3
  • methyl-CpG binding domain protein 1 isoform PCM1
  • methyl-CpG binding domain protein 1
  • methyl-CpG-binding domain protein 1
  • Methyl-CpG-binding protein MBD1
  • PCM1CXXC3RFT
  • Protein containing methyl-CpG-binding domain 1
  • the regulator of fibroblast growth factor 2 (FGF-2) transcription

Background

DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBD1 Antibody (NB100-56537)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, IHC-Fr, IP, WB.


Filter By Application
ChIP
(1)
IHC-Fr
(1)
IP
(2)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-56537 Applications Species
Varshney D, Vavrova-Anderson J, Oler AJ et al. SINE transcription by RNA polymerase III is suppressed by histone methylation but not by DNA methylation Nat Commun 2015 Mar 24 [PMID: 25798578] (WB, ChIP, Human, Mouse) WB, ChIP Human, Mouse
Hendriks IA, Treffers LW, Verlaan-de Vries M et al. SUMO-2 Orchestrates Chromatin Modifiers in Response to DNA Damage Cell Rep 2015 Mar 10 [PMID: 25772364] (WB, Human) WB Human
Waterfield M, Khan IS, Cortez JT et al. The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance. Nat. Immunol. 2014 Mar 1 [PMID: 24464130] (WB, IP, IHC-Fr, Mouse, Human)

Details:
IP/WB (HEk293), Figs 1C, S2c; IHC-F(mouse thymic medulla), Fig D.
WB, IP, IHC-Fr Mouse, Human
Kumar Deepak, Verma Mukesh. Methods in cancer epigenetics and epidemiology. Methods Mol Biol. 2009 [PMID: 19109785] (Human) Human
Villa R, Pasini D, Gutierrez A et al. Role of the polycomb repressive complex 2 in acute promyelocytic leukemia. Cancer Cell. 2007 Jun [PMID: 17560333]
Ruddock-D'Cruz NT, Xue J, Wilson KJ et al. Dynamic changes in the localization of five members of the methyl binding domain (MBD) gene family during murine and bovine preimplantation embryo development. Mol Reprod Dev. 2008 Jan [PMID: 17546630] (WB, Mouse)

Details:
IF [bovine embryos (Fig 3) and mouse embryos (Fig 4)] and for WB [bovine heart, (supplementary data)] 1. MBD1 mAb (IMG-306) 2. MBD3/MBD2 mAb (IMG-296A) 3. MBD4 pAb (IMG-285).
WB Mouse
Sarraf Shireen A, Stancheva Irina. Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell. 2004 Aug 27 [PMID: 15327775] (IP, Human) IP Human
Reese BE, Bachman KE, Baylin SB, Rountree MR. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol. 2003 May [PMID: 12697822]

Review for MBD1 Antibody (NB100-56537) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56537:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MBD1 NB100-56537
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedSample tested: Jurkat cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Mouse IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7570
Secondary ConcentrationDiluted 5,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for MBD1 Antibody (NB100-56537) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56537AF405
Alexa Fluor 488 Labeled NB100-56537AF488
Alexa Fluor 647 Labeled NB100-56537AF647
Alexa Fluor 700 Labeled NB100-56537AF700
Biotin Labeled NB100-56537B
DyLight 350 Labeled NB100-56537UV
DyLight 405 Labeled NB100-56537V
DyLight 488 Labeled NB100-56537G
DyLight 550 Labeled NB100-56537R
DyLight 650 Labeled NB100-56537C
DyLight 680 Labeled NB100-56537FR
DyLight 755 Labeled NB100-56537IR
HRP Labeled NB100-56537H

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Recent Reviews

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5
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4
0
3
1
2
0
1
0

Anonymous
10/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol MBD1
Entrez
Uniprot