Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody

Images

 
Biological Strategies: Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - KDM6A protein levels are decreased following EMT and upregulated during MET. MCF10A cells, treated as ...read more
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + ...read more
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Analysis of JMJD3 expression on HepG2 whole cell lysates.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse JMJD3 protein (within residues 600-650). [Swiss-Prot# Q5NCY0]
Localization
Nucleus
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM6B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This JMJD3 antibody is useful for Western blot where a band is seen at ~180 kDa. Use in Flow Cytometry was reported in scientific literature (PMID: 23772023).
Control
HepG2 Whole Cell Lysate
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Knockout 293T Cell Lysate
Control Peptide
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Peptide (NBP1-06640PEP)
Reviewed Applications
Read 4 Reviews rated 4.3
using
NBP1-06640 in the following applications:

Publications
Read Publications using
NBP1-06640 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JmjC domain-containing protein 3
  • JMJD3
  • JMJD3lysine-specific demethylase 6B
  • jumonji domain containing 3, histone lysine demethylase
  • Jumonji domain-containing protein 3
  • KDM6B
  • KIAA0346jumonji domain containing 3
  • Lysine (K)specific Demethylase 6B
  • Lysine (K)-specific Demethylase 6B
  • Lysine demethylase 6B

Background

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-81657
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, ChIP
NBP1-30141
Species: Hu, Mu, Rt, Po, Ce, Dr, In, Ma, Ye
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-80628
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-83976
Species: Hu, Mu, Pm
Applications: WB
NB100-56664
Species: Hu, Mu
Applications: WB, ChIP, IHC, IHC-P
NBP1-43299
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP3-03738
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, KO
NBP2-27293
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB6798
Species: Hu
Applications: WB, ICC
NB100-1045
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-82455
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, ICC/IF, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-06640 Applications Species
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J. Clin. Invest. May 19 2020 [PMID: 32427586] (WB, Human) WB Human
Huang M, Wang Q, Long F et al. Jmjd3 regulates inflammasome activation and aggravates DSS-induced colitis in mice FASEB J. Jan 23 2020 [PMID: 31971317] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Luo X, Yang D, Wu W et al. Critical role of histone demethylase JMJD3 in the regulation of neointima formation following vascular injury. Cardiovasc. Res. Jul 4 2018 [PMID: 29982434]
Liu S, Wang X, Pan L, Wu W et al. Endogenous hydrogen sulfide regulates histone demethylase JMJD3-mediated inflammatory response in LPS-stimulated macrophages and in a mouse model of LPS-induced septic shock Biochem. Pharmacol. 2017 Oct 23 [PMID: 29074105] (WB, Mouse) WB Mouse
Taube JH, Sphyris N, Johnson KS et al. The H3K27me3-demethylase KDM6A is suppressed in breast cancer stem-like cells, and enables the resolution of bivalency during the mesenchymal-epithelial transition. Oncotarget. 2017 Sep 12 [PMID: 29029452] (WB, Human) WB Human
Luo T, Dunphy PS, McBride JW. Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. Front Cell Infect Microbiol May 29 2017 [PMID: 28553621] (WB, Human) WB Human
Pham D, Yu Q, Walline CC et al. Opposing Roles of STAT4 and Dnmt3a in Th1 Gene Regulation. J Immunol 2013 Jul 15 [PMID: 23772023] (FLOW, Mouse) FLOW Mouse

Reviews for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse and Human, Other.

Reviews using NBP1-06640:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(2)
Mouse and Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP1-06640
Enlarge
5
reviewed by:
Robin Ren
WB Mouse and Human 10/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate, Sample Amount: 50ug-100ug protein
SpeciesMouse and Human
LotF-3
  4
reviewed by:
Anonymous
WB Human 09/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol
  4
reviewed by:
Anonymous
WB Other 06/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedPC12 whole cell lysate
SpeciesOther
LotB

Blocking

Blocking Details5% milk in PBST for 1 hour at room temperature

Primary Anitbody

Dilution Ratio1:1000 in 1% BSA at 4oC overnight

Secondary Antibody

Secondary DescriptionGoat anti rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling #7074
Secondary Concentration1:2000

Details

Detection NotesBio-Rad Clarity Western ECL Substrate 2 minutes
Western Blot Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP1-06640
Enlarge
4
reviewed by:
Andy Martens
WB Human 11/16/2012
View

Summary

ApplicationWestern Blot
Sample Tested293T cells; lane 3 transfected with an miRNA
SpeciesHuman
LotB
CommentsThe antibody looked nearly identical to the example pic on the website; was able to see the JMJD3 band. I will need to adjust some concentrations and dilutions if/when we do it again.

Blocking

Blocking DetailsPBS-T with 5% milk 2 hrs

Primary Anitbody

Dilution RatioPrimary 2 ug/ml

Secondary Antibody

Secondary Descriptionmouse anti-rabbit IgG HR
Secondary Manufacturer Cat#Santa Cruz sc-2357
Secondary Concentration1:2000

Details

Detection NotesLane one neg control, lane two empty vector.

Comments

CommentsThe antibody looked nearly identical to the example pic on the website; was able to see the JMJD3 band. I will need to adjust some concentrations and dilutions if/when we do it again.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640). (Showing 1 - 1 of 1 FAQs).

  1. A customer is interested in using the KDM6B antibody, cat# NBP1-06640, with rat samples. Could you please BLAST the immunogen against the rat sequence, found on UniProt below: http://www.uniprot.org/uniprot/G3V9U4
    • This antibody shares 100% sequence homology with the rat protein. Since this is higher that 90%, the species will be covered in our guarantee.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-06640AF350
Alexa Fluor 405 NBP1-06640AF405
Alexa Fluor 488 NBP1-06640AF488
Alexa Fluor 532 NBP1-06640AF532
Alexa Fluor 594 NBP1-06640AF594
Alexa Fluor 647 NBP1-06640AF647
Alexa Fluor 700 NBP1-06640AF700
Alexa Fluor 750 NBP1-06640AF750
Biotin NBP1-06640B
DyLight 350 NBP1-06640UV
DyLight 405 NBP1-06640V
DyLight 488 NBP1-06640G
DyLight 550 NBP1-06640R
DyLight 594 NBP1-06640DL594
DyLight 650 NBP1-06640C
DyLight 680 NBP1-06640FR
DyLight 755 NBP1-06640IR
FITC NBP1-06640F
HRP NBP1-06640H
Janelia Fluor 549 NBP1-06640JF549
Janelia Fluor 646 NBP1-06640JF646

Additional Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Products

Bioinformatics Tool for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

Discover related pathways, diseases and genes to Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

Discover more about diseases related to Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640).
 

Pathways for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

View related products by pathway.

PTMs for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

Learn more about PTMs related to Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640).
 

Research Areas for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

Find related products by research area.

Blogs on Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3

There are no specific blogs for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Robin Ren
10/30/2018
Application: WB
Species: Mouse and Human

 
Anonymous
09/07/2015
Application: WB
Species: Human

 
Anonymous
06/25/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol KDM6B