Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

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Biological Strategies: Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - KDM6A protein levels are decreased following EMT and upregulated during MET. MCF10A cells, treated as ...read more
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + ...read more
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Analysis of JMJD3 expression on HepG2 whole cell lysates.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of the mouse JMJD3 protein (within residues 600-650). [Swiss-Prot# Q5NCY0]
Localization
Nucleus
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM6B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 2 ug/ml
Application Notes
Use in IHC reported in scientific literature (PMID:35177643) This JMJD3 antibody is useful for Western blot where a band is seen at ~180 kDa. Use in Flow Cytometry was reported in scientific literature (PMID: 23772023)..
Control
HepG2 Whole Cell Lysate
Control Peptide
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Peptide (NBP1-06640PEP)
Reviewed Applications
Read 4 Reviews rated 4.3
using
NBP1-06640 in the following applications:

Publications
Read Publications using
NBP1-06640 in the following applications:

  • 1 publication
  • 1 publication
  • IHC
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

  • EC 1.14.11
  • EC 1.14.11.-
  • JmjC domain-containing protein 3
  • JMJD3
  • JMJD3lysine-specific demethylase 6B
  • jumonji domain containing 3, histone lysine demethylase
  • Jumonji domain-containing protein 3
  • KDM6B
  • KIAA0346jumonji domain containing 3
  • Lysine (K)specific Demethylase 6B
  • Lysine (K)-specific Demethylase 6B
  • Lysine demethylase 6B

Background

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IHC
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-06640 Applications Species
Ming-Chin Lee K, Achuthan AA, De Souza DP et al. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization Cell reports Apr 19 2022 [PMID: 35443173] (WB) WB
Wang W, Cho H, Lee JW, Lee SK The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development Nature communications Feb 17 2022 [PMID: 35177643] (IHC, Mouse) IHC Mouse
Wu Q, Shichino Y, Abe T et al. Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells Nature communications Jan 25 2022 [PMID: 35078993] (WB, Mouse) WB Mouse
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J. Clin. Invest. May 19 2020 [PMID: 32427586] (WB, Human) WB Human
Huang M, Wang Q, Long F et al. Jmjd3 regulates inflammasome activation and aggravates DSS-induced colitis in mice FASEB J. Jan 23 2020 [PMID: 31971317] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Luo X, Yang D, Wu W et al. Critical role of histone demethylase JMJD3 in the regulation of neointima formation following vascular injury. Cardiovasc. Res. Jul 4 2018 [PMID: 29982434]
Liu S, Wang X, Pan L, Wu W et al. Endogenous hydrogen sulfide regulates histone demethylase JMJD3-mediated inflammatory response in LPS-stimulated macrophages and in a mouse model of LPS-induced septic shock Biochem. Pharmacol. 2017 Oct 23 [PMID: 29074105] (WB, Mouse) WB Mouse
Taube JH, Sphyris N, Johnson KS et al. The H3K27me3-demethylase KDM6A is suppressed in breast cancer stem-like cells, and enables the resolution of bivalency during the mesenchymal-epithelial transition. Oncotarget. 2017 Sep 12 [PMID: 29029452] (WB, Human) WB Human
Luo T, Dunphy PS, McBride JW. Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. Front Cell Infect Microbiol May 29 2017 [PMID: 28553621] (WB, Human) WB Human
Pham D, Yu Q, Walline CC et al. Opposing Roles of STAT4 and Dnmt3a in Th1 Gene Regulation. J Immunol 2013 Jul 15 [PMID: 23772023] (FLOW, Mouse) FLOW Mouse

Reviews for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Human and Mouse, Other.

Reviews using NBP1-06640:
Filter by Applications
Western Blot
(4)
All Applications
Filter by Species
Human
(2)
Human and Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP1-06640
Enlarge
5
reviewed by:
Verified Customer
Western Blot Human and Mouse 10/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate, Sample Amount: 50ug-100ug protein
SpeciesHuman and Mouse
LotF-3
  4
reviewed by:
Haiquan Lu
Western Blot Human 09/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman
  4
reviewed by:
Ning Wang
Western Blot Other 06/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedPC12 whole cell lysate
SpeciesOther
LotB
Western Blot Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP1-06640
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 11/16/2012
View

Summary

ApplicationWestern Blot
Sample Tested293T cells; lane 3 transfected with an miRNA
SpeciesHuman
LotB

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640). (Showing 1 - 1 of 1 FAQs).

  1. A customer is interested in using the KDM6B antibody, cat# NBP1-06640, with rat samples. Could you please BLAST the immunogen against the rat sequence, found on UniProt below: http://www.uniprot.org/uniprot/G3V9U4
    • This antibody shares 100% sequence homology with the rat protein. Since this is higher that 90%, the species will be covered in our guarantee.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

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Diseases for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640)

Discover more about diseases related to Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody (NBP1-06640).
 

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Verified Customer
10/30/2018
Application: Western Blot
Species: Human and Mouse

 
Haiquan Lu
09/07/2015
Application: Western Blot
Species: Human

 
Ning Wang
06/25/2014
Application: Western Blot
Species: Other

Bioinformatics

Gene Symbol KDM6B