M-CSFR/CD115 Antibody - BSA Free

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human skeletal muscle shows low positivity in myocytes as expected.

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human lymph node shows moderate cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody [NBP1-84532] - Staining of human rectum shows weak to moderate cytoplasmic positivity in lymphoid cells.

Staining of human cell line A-431 shows localization to plasma membrane & vesicles.

Analysis in human cell line BEWO.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

M-CSFR/CD115 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CSF1R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  M-CSFR/CD115 Recombinant Protein Antigen (NBP1-84532PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for M-CSFR/CD115 Antibody - BSA Free

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF1R
• Entrez:
  • 1436(Human)
• Uniprot:
  • P07333(Human)