Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 66903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Flt‑3/Flk‑2
  Asn27-Asn541
  Accession # AAA18947
• Specificity: Detect human Flt‑3/Flk‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Flt-3, recombinant human (rh) SCF R, rhPDGF R alpha, or rhPDGF R beta is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT3

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
2. Drexler, H.G. (1996) Leukemia 10:588.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3/Flk-2/CD135 Antibody (MAB812)(1)

Publication using MAB812

• Williams A, Li L, Nguyen B, Brown P, Levis M, Small D Fluvastatin inhibits FLT3 glycosylation in human and murine cells and prolongs survival of mice with FLT3/ITD leukemia. Blood, 2012;120(15):3069-79. 2012 [PMID: 22927251] (ICC, Mouse)

Bioinformatics

• Gene Symbol: FLT3
• Uniprot:
  • AAA18947()