Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)


1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 0.5-2 ng/mL.

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Applications Bioactivity

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Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Summary

Details of Functionality
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.5‑2 ng/mL.
E. coli-derived mouse TRANCE/TNFSF11/RANK L protein
Lys158-Asp316, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
462-TEC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

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Publications for TRANCE/TNFSF11/RANK L (462-TEC)(74)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, Differentiation.

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Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using 462-TEC Applications Species
H Choi, L Yang, Y Liu, JK Jeong, ES Cho Npp1 prevents external tooth root resorption by regulation of cervical cementum integrity Scientific Reports, 2022;12(1):21158. 2022 [PMID: 36477209] (Cell Culture, Mouse) Cell Culture Mouse
Y Zhang, M Yang, S Zhang, Z Yang, Y Zhu, Y Wang, Z Chen, X Lv, Z Huang, Y Xie, L Cai BHLHE40 promotes osteoclastogenesis and abnormal bone resorption via c-Fos/NFATc1 Cell & bioscience, 2022;12(1):70. 2022 [PMID: 35619122] (Bioassay, Mouse) Bioassay Mouse
P Cai, S Yan, Y Lu, X Zhou, X Wang, M Wang, Z Yin Carnosol inhibits osteoclastogenesis in�vivo and in�vitro by blocking the RANKL?induced NF?kappaB signaling pathway Molecular Medicine Reports, 2022;26(1):. 2022 [PMID: 35593299] (Bioassay, Mouse) Bioassay Mouse
Z Cui, C Feng, J Chen, Y Wang, Q Meng, S Zhao, Y Zhang, D Feng, Z Li, S Sun Network Pharmacology Deciphers the Action of Bioactive Polypeptide in Attenuating Inflammatory Osteolysis via the Suppression of Oxidative Stress and Restoration of Bone Remodeling Balance Oxidative Medicine and Cellular Longevity, 2022;2022(0):4913534. 2022 [PMID: 35578727] (Bioassay, Mouse) Bioassay Mouse
Y Ma, R Di, H Zhao, R Song, H Zou, Z Liu P2X7 receptor knockdown suppresses osteoclast differentiation by inhibiting autophagy and Ca2+/calcineurin signaling Molecular Medicine Reports, 2022;25(5):. 2022 [PMID: 35266012] (Cell Culture, Mouse) Cell Culture Mouse
C Yang, H Tao, H Zhang, Y Xia, J Bai, G Ge, W Li, W Zhang, L Xiao, Y Xu, Z Wang, Y Gu, H Yang, Y Liu, D Geng TET2 regulates osteoclastogenesis by modulating autophagy in OVX-induced bone loss Autophagy, 2022;0(0):. 2022 [PMID: 35255774] (Cell Culture, Mouse) Cell Culture Mouse
JM Kim, YS Yang, J Xie, O Lee, J Kim, J Hong, B Boldyreff, O Filhol, H Chun, MB Greenblatt, G Gao, JH Shim Regulation of sclerostin by the SIRT1 stabilization pathway in osteocytes Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35169297] (Bioassay, Mouse) Bioassay Mouse
ANM Mamun-Or-R, TT Lucy, M Yagi, Y Yonei Inhibitory Effects of Astaxanthin on CML-HSA-Induced Inflammatory and RANKL-Induced Osteoclastogenic Gene Expression in RAW 264.7 Cells Biomedicines, 2021;10(1):. 2021 [PMID: 35052734] (Bioassay, Mouse) Bioassay Mouse
J Gu, X Zhang, C Zhang, Y Li, J Bian, X Liu, Y Yuan, H Zou, X Tong, Z Liu Galectin-3 Contributes to the Inhibitory Effect of lalpha,25-(OH)2D3 on Osteoclastogenesis International Journal of Molecular Sciences, 2021;22(24):. 2021 [PMID: 34948130] (Bioassay, Mouse) Bioassay Mouse
S Zhou, Q Dai, X Huang, A Jin, Y Yang, X Gong, H Xu, X Gao, L Jiang STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis Nature Communications, 2021;12(1):6891. 2021 [PMID: 34824272] (Bioassay, Mouse) Bioassay Mouse
Show All 74 Publications.

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol Tnfsf11