Recombinant Human SCF Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human SCF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
KITLG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-18 kDa, under reducing condtitions
Publications
Read Publications using
255-SC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF Protein

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

What is Stem cell factor?

Stem cell factor (SCF) is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. SCF protein promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). And SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (9). Stem cell factor is an important cytokine for ex vivo clinical applications. Along with other cytokines, SCF is used in the culture and expansion of hematopoietic stem cells (HSCs) as well as for proliferation and differentiation of both myeloid and erythroid progenitor cells.

Mature stem cell factor consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane domain, and a 36 aa cytoplasmic tail (10). The ECD shows both N linked and O-linked glycosylation (11). SCF protein exists in two forms, a membrane-bound form and a proteolytically processed soluble form that lacks the transmembrane domain and cytoplasmic tail. The soluble form is created by proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releasing a 25 kDa soluble SCF protein which is comparable to the only form produced by Steel-dickie mutant mice (12, 13). There is also an alternately spliced isoform of human SCF that lacks 28 amino acids that encompasses the primary proteolytic recognition site (14). This form cannot be cleaved and is only membrane bound.

SCF binds to C-kit (CD117). C-kit is expressed on many different cell types including HSCs, mast cells, germ cells, and melanocytes. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain (15). Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. Stem cell factor is highly conserved among mammals. Human SCF protein shares 79% 87% aa sequence identity with dog, cat, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. 
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. 
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287. 
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. 
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
  9. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  10. Martin, F.H. et al. (1990) Cell 63:203.
  11. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  12. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  13. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
  14. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

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Publications for SCF/c-kit Ligand (255-SC)(191)

We have publications tested in 7 confirmed species: Human, Mouse, Hamster, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio anubis (Olive Baboon).

We have publications tested in 4 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard).


Filter By Application
Bioassay
(171)
Cell Culture
(19)
Differentiation
(2)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(178)
Mouse
(5)
Hamster
(1)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Papio anubis (Olive Baboon)
(1)
All Species
Showing Publications 1 - 10 of 191. Show All 191 Publications.
Publications using 255-SC Applications Species
Mayuranathan, T;Newby, GA;Feng, R;Yao, Y;Mayberry, KD;Lazzarotto, CR;Li, Y;Levine, RM;Nimmagadda, N;Dempsey, E;Kang, G;Porter, SN;Doerfler, PA;Zhang, J;Jang, Y;Chen, J;Bell, HW;Crossley, M;Bhoopalan, SV;Sharma, A;Tisdale, JF;Pruett-Miller, SM;Cheng, Y;Tsai, SQ;Liu, DR;Weiss, MJ;Yen, JS; Potent and uniform fetal hemoglobin induction via base editing Nature genetics 2023-07-03 [PMID: 37400614] (Cell Culture, Human) Cell Culture Human
A Gehrlein, V Udayar, N Anastasi, ML Morella, I Ruf, D Brugger, S von der Ma, R Thoma, A Rufer, D Heer, N Pfahler, A Jochner, J Niewoehner, L Wolf, M Fueth, M Ebeling, R Villaseñor, Y Zhu, MC Deen, X Shan, Z Ehsaei, V Taylor, E Sidransky, DJ Vocadlo, PO Freskgård, R Jagasia Targeting neuronal lysosomal dysfunction caused by beta-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct Nature Communications, 2023-04-12;14(1):2057. 2023-04-12 [PMID: 37045813] (Bioassay, Human) Bioassay Human
AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G&gt;A (p.D249N) autosomal dominant mutation in TUBB4A Stem Cell Research, 2023-03-26;69(0):103083. 2023-03-26 [PMID: 37003180] (Bioassay, Human) Bioassay Human
S Li, CS Wang, A Montel-Hag, HC Chen, S Lopez, O Zhou, K Dai, S Tsai, W Satyadi, C Botero, C Wong, D Casero, GM Crooks, CS Seet Strength of CAR signaling determines T�cell versus ILC differentiation from pluripotent stem cells Cell Reports, 2023-03-11;42(3):112241. 2023-03-11 [PMID: 36906850] (Bioassay, Human) Bioassay Human
Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung&#039;s disease Cell Stem Cell, 2023-03-02;30(3):264-282.e9. 2023-03-02 [PMID: 36868194] (Bioassay, Human) Bioassay Human
Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung&#039;s disease Cell Stem Cell, 2023;30(3):264-282.e9. 2023 [PMID: 36868194] (Bioassay, Human) Bioassay Human
L Feige, T Kozaki, G Dias de Me, V Guillemot, F Larrous, F Ginhoux, H Bourhy Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses Viruses, 2022-12-29;15(1):. 2022-12-29 [PMID: 36680128] (Bioassay, Human) Bioassay Human
JP Borges, RSR Sætra, A Volchuk, M Bugge, P Devant, B Sporsheim, BR Kilburn, CL Evavold, JC Kagan, NM Goldenberg, TH Flo, BE Steinberg Glycine inhibits NINJ1 membrane clustering to suppress plasma membrane rupture in cell death Elife, 2022-12-05;11(0):. 2022-12-05 [PMID: 36468682] (Cell Culture, Mouse) Cell Culture Mouse
PY Su, W Lee, J Zernant, SH Tsang, T Nagasaki, B Corneo, R Allikmets Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture Oncogene, 2022-11-14;65(0):102973. 2022-11-14 [PMID: 36455383] (Bioassay, Human) Bioassay Human
X Liu, N Sato, T Yabushita, J Li, Y Jia, M Tamura, S Asada, T Fujino, T Fukushima, T Yonezawa, Y Tanaka, T Fukuyama, A Tsuchiya, S Shikata, H Iwamura, C Kinouchi, K Komatsu, S Yamasaki, T Shibata, AT Sasaki, J Schibler, M Wunderlich, E O'Brien, B Mizukawa, JC Mulloy, Y Sugiura, H Takizawa, T Shibata, K Miyake, T Kitamura, S Goyama IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia Embo Molecular Medicine, 2022-12-01;0(0):e15631. 2022-12-01 [PMID: 36453131] (Cell Culture, Human) Cell Culture Human
Show All 191 Publications.

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Bioinformatics

Gene Symbol KITLG
Uniprot