Recombinant Human SCF Protein

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1 μg/lane of Recombinant Human SCF/c-kit Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human SCF Protein Summary

Additional Information
A New rhSCF is Now Available! It has a lower endotoxin spec and is human cell expressed!
New Product 7466-SC
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.
The specific activity of Recombinant Human SCF is approximately 564 IU/μg, which is calibrated against recombinant human SCF WHO International Standard (NIBSC code: 91/682).
Source
E. coli-derived
Glu26-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
KITLG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
255-SC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF Protein

  • c-kit Ligand
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

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Publications for (102)

We have publications tested in 8 confirmed species: Human, Mouse, Hamster, N/A, Porcine, Primate - Baboon, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Bioassay, ELISA Standard.


Filter By Application
Bioassay
(99)
ELISA Standard
(1)
All Applications
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Human
(89)
Mouse
(1)
Hamster
(1)
N/A
(1)
Porcine
(1)
Primate - Baboon
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
All Species
Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using 255-SC Applications Species
NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis Cell Rep, 2017;20(7):1597-1608. 2017 [PMID: 28813672] (Bioassay, Human) Bioassay Human
L Butcher, M Ahluwalia, T Örd, J Johnston, RH Morris, E Kiss-Toth, T Örd, JD Erusalimsk Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis Sci Rep, 2017;7(1):6684. 2017 [PMID: 28751721] (Bioassay, Human) Bioassay Human
J Helft, F Anjos-Afon, AG van der Ve, P Chakravart, D Bonnet, C Reis E Sou Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors Cell Rep, 2017;20(3):529-537. 2017 [PMID: 28723558] (Bioassay, Human) Bioassay Human
E Varga, C Nemes, I Bock, Z Táncos, S Berzsenyi, G Lévay, V Román, J Kobolák, A Dinnyés Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD) Stem Cell Res, 2017;21(0):19-22. 2017 [PMID: 28677532] (Bioassay, Human) Bioassay Human
J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 2017;5(0):241-258. 2017 [PMID: 28603745] (Bioassay, Human) Bioassay Human
H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28522588] (Bioassay, Human) Bioassay Human
S Liu, Z Ye, Y Gao, C He, DW Williams, A Moliterno, J Spivak, H Huang, L Cheng Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene Stem Cell Res, 2017;18(0):57-59. 2017 [PMID: 28395806] (Bioassay, Human) Bioassay Human
K Trakarnsan, RE Griffiths, MC Wilson, A Blair, TJ Satchwell, M Meinders, N Cogan, S Kupzig, R Kurita, Y Nakamura, AM Toye, DJ Anstee, J Frayne An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells Nat Commun, 2017;8(0):14750. 2017 [PMID: 28290447] (Bioassay, Human) Bioassay Human
AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28215825] (Bioassay, Human) Bioassay Human
I Baccelli, J Krosl, G Boucher, I Boivin, VP Lavallée, J Hébert, S Lemieux, A Marinier, G Sauvageau A novel approach for the identification of efficient combination therapies in primary human acute myeloid leukemia specimens Blood Cancer J, 2017;7(2):e529. 2017 [PMID: 28211886] (Bioassay, Human) Bioassay Human
Show All 102 Publications.

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Bioinformatics

Gene Symbol KITLG
Entrez
Uniprot