Recombinant Human SCF Protein


1 μg/lane of Recombinant Human SCF/c-kit Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human SCF Protein Summary

Additional Information
A New rh SCF is Now Available! It has a lower endotoxin specification and is human cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.
The specific activity of Recombinant Human SCF is approximately 564 IU/μg, which is calibrated against recombinant human SCF WHO International Standard (NIBSC code: 91/682).
E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
255-SC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF Protein

  • c-kit Ligand
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • KIT ligand
  • Kitl
  • KL-1
  • Mast cell growth factor
  • MGF
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SLF
  • steel factor


Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28‑40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1‑7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N‑linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the long isoform (corresponding to this recombinant protein), human SCF shares 79%‑87% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (9). Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c‑kit to trigger receptor dimerization and signaling (14). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (15).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. 
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. 
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287. 
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. 
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
  9. Martin, F.H. et al. (1990) Cell 63:203.
  10. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  11. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  12. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
  13. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
  14. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
  15. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

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Publications for SCF/c-kit Ligand (255-SC)(120)

We have publications tested in 8 confirmed species: Human, Mouse, Hamster, N/A, Porcine, Primate - Baboon, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Bioassay, ELISA Standard.

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Primate - Baboon
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 120. Show All 120 Publications.
Publications using 255-SC Applications Species
N Uchida, T Nassehi, CM Drysdale, J Gamer, M Yapundich, S Demirci, JJ Haro-Mora, A Leonard, MM Hsieh, JF Tisdale High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2 Mol Ther Methods Clin Dev, 2019;13(0):187-196. 2019 [PMID: 30788387] (Bioassay, Human) Bioassay Human
X Wang, T Liao, C Wan, X Yang, J Zhao, R Fu, Z Yao, Y Huang, Y Shi, G Chang, Y Zheng, F Luo, Z Liu, Y Wang, X Mao, XY Zhao Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale PeerJ, 2019;6(0):e6143. 2019 [PMID: 30643676] (Bioassay, Human) Bioassay Human
S Poldee, C Metheetrai, S Nugoolsuks, J Frayne, K Trakarnsan Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells MethodsX, 2018;5(0):1626-1632. 2018 [PMID: 30560064] (Bioassay, Human) Bioassay Human
A Minagawa, T Yoshikawa, M Yasukawa, A Hotta, M Kunitomo, S Iriguchi, M Takiguchi, Y Kassai, E Imai, Y Yasui, Y Kawai, R Zhang, Y Uemura, H Miyoshi, M Nakanishi, A Watanabe, A Hayashi, K Kawana, T Fujii, T Nakatsura, S Kaneko Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30449714] (Bioassay, Human) Bioassay Human
A Camgoz, M Paszkowski, S Satpathy, M Wermke, MV Hamann, M von Bonin, C Choudhary, S Knapp, F Buchholz STK3 is a therapeutic target for a subset of acute myeloid leukemias Oncotarget, 2018;9(39):25458-25473. 2018 [PMID: 29876001] (Bioassay, Human) Bioassay Human
N Ueda, Y Uemura, R Zhang, S Kitayama, S Iriguchi, Y Kawai, Y Yasui, M Tatsumi, T Ueda, TY Liu, Y Mizoro, C Okada, A Watanabe, M Nakanishi, S Senju, Y Nishimura, K Kuzushima, H Kiyoi, T Naoe, S Kaneko Generation of TCR-Expressing Innate Lymphoid-like Helper Cells that Induce Cytotoxic T Cell-Mediated Anti-leukemic Cell Response Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29805109] (Bioassay, Human) Bioassay Human
P Shen, S Yang, H Sun, G Li, B Wu, F Ji, T Sun, D Zhou SCF/c-KIT Signaling Increased Mucin2 Production by Maintaining Atoh1 Expression in Mucinous Colorectal Adenocarcinoma Int J Mol Sci, 2018;19(5):. 2018 [PMID: 29786668] (Bioassay, Human) Bioassay Human
MC Allenby, A Tahlawi, JCF Morais, K Li, N Panoskalts, A Mantalaris Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids Stem Cells Int, 2018;2018(0):6230214. 2018 [PMID: 29760729] (Bioassay, Human) Bioassay Human
M Cesana, MH Guo, D Cacchiarel, L Wahlster, J Barragan, S Doulatov, LT Vo, B Salvatori, C Trapnell, K Clement, P Cahan, KM Tsanov, PM Sousa, B Tazon-Vega, A Bolondi, FM Giorgi, A Califano, JL Rinn, A Meissner, JN Hirschhorn, GQ Daley A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development Cell Stem Cell, 2018;22(4):575-588.e7. 2018 [PMID: 29625070] (Bioassay, Human) Bioassay Human
S Kotha, S Sun, A Adams, B Hayes, KT Phong, R Nagao, JA Reems, D Gao, B Torok-Stor, JA López, Y Zheng Microvasculature-directed thrombopoiesis in a 3D in vitro marrow microenvironment PLoS ONE, 2018;13(4):e0195082. 2018 [PMID: 29617409] (Bioassay, Human) Bioassay Human
Show All 120 Publications.

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Gene Symbol KITLG