PDGF R beta Antibody [Unconjugated]

Images

 
Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal ...read more
PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) ...read more
Expression of sFlt1 in pericytes at the angiogenic front. a Maximum intensity projections of confocal images from P6 retinas of the Hey1-GFP transgenic reporter mouse model stained for GFP (green), PDGFR beta + (white) ...read more
VCAM1 is involved in the regulation of proliferation and morphological changes in oligodendrocytes.(a) Tissue lysates from 7-day-old NG2-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Ng2-Cre) or control (Ctrl) ...read more
Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

PDGF R beta Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta
Leu32-Lys530
Accession # P05622
Specificity
Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRB
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1042 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta Antibody [Unconjugated]

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK12
  • PDGF R beta
  • PDGFR
  • PDGFR1
  • PDGFRB
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
AF1062
Species: Mu
Applications: IHC, Neut, WB
AF644
Species: Mu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
DVE00
Species: Hu
Applications: ELISA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AF5414
Species: Hu
Applications: Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB1230
Species: Hu, Mu, Rt
Applications: ICC, IHC, KO, Simple Western, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP1-58279
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-80739
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, IHC-P, WB
221-AA
Species: Hu
Applications: BA
236-EG
Species: Hu
Applications: BA
NBP2-19804
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-88937
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB

Publications for PDGF R beta Antibody (AF1042)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse.

We have publications tested in 9 applications: Confocal Imaging, Flow Cytometry, ICC, IHC, IHC-F, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Confocal Imaging
(1)
Flow Cytometry
(3)
ICC
(4)
IHC
(34)
IHC-F
(1)
IHC-Fr
(4)
IHC-P
(6)
Neutralization
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(48)
Rat
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using AF1042 Applications Species
Esposito, E;Licastro, E;Cuomo, O;Lo, EH;Hayakawa, K;Pignataro, G; Postconditioning promotes recovery in the neurovascular unit after stroke Frontiers in cellular neuroscience 2023-09-08 [PMID: 37744881] (IHC, Rat) IHC Rat
Englert, N;Burkard, P;Aue, A;Rosenwald, A;Nieswandt, B;Friebe, A; Anti-Fibrotic and Anti-Inflammatory Role of NO-Sensitive Guanylyl Cyclase in Murine Lung International journal of molecular sciences 2023-07-19 [PMID: 37511420] (IHC, Mouse) IHC Mouse
Biswas, S;Shahriar, S;Bachay, G;Arvanitis, P;Brunken, WJ;Agalliu, D; Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/ beta -catenin signaling bioRxiv : the preprint server for biology 2023-07-11 [PMID: 37503079] (IHC, Transgenic Mouse, Mouse) IHC Transgenic Mouse, Mouse
Aue, A;Englert, N;Harrer, L;Schwiering, F;Gaab, A;König, P;Adams, R;Schmidtko, A;Friebe, A;Groneberg, D; NO-sensitive guanylyl cyclase discriminates pericyte-derived interstitial from intra-alveolar myofibroblasts in murine pulmonary fibrosis Respiratory research 2023-06-22 [PMID: 37349733] (IHC, Mouse) IHC Mouse
Bennett, HC;Zhang, Q;Wu, YT;Chon, U;Pi, HJ;Drew, PJ;Kim, Y; Aging drives cerebrovascular network remodeling and functional changes in the mouse brain bioRxiv : the preprint server for biology 2023-05-24 [PMID: 37305850] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Oliveira, F;Bondareva, O;Rodr�guez-Aguilera, JR;Sheikh, BN; Cultured brain pericytes adopt an immature phenotype and require endothelial cells for expression of canonical markers and ECM genes Frontiers in cellular neuroscience 2023-05-02 [PMID: 37201162] (ICC, Mouse) ICC Mouse
LB Payne, H Abdelazim, M Hoque, A Barnes, Z Mironovova, CE Willi, J Darden, C Jenkins-Ho, MW Sedovy, SR Johnstone, JC Chappell A Soluble Platelet-Derived Growth Factor Receptor-beta Originates via Pre-mRNA Splicing in the Healthy Brain and is Differentially Regulated during Hypoxia and Aging bioRxiv : the preprint server for biology, 2023-02-04;0(0):. 2023-02-04 [PMID: 36778261] (Western Blot, Mouse) Western Blot Mouse
N Romanò, C Lafont, P Campos, A Guillou, T Fiordelisi, DJ Hodson, P Mollard, M Schaeffer Median eminence blood flow influences food intake by regulating ghrelin access to the metabolic brain JCI Insight, 2023-02-08;0(0):. 2023-02-08 [PMID: 36574295] (Confocal Imaging, Mouse) Confocal Imaging Mouse
Z Li, S Yu, Y Liu, X Hu, Y Li, Z Xiao, Y Chen, D Tian, X Xu, L Cheng, M Zheng, J Jing SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRbeta pathway Journal of Neuroinflammation, 2022-04-16;19(1):95. 2022-04-16 [PMID: 35429978] (IHC, Mouse) IHC Mouse
Q Zhao, W Dai, HY Chen, RE Jacobs, BV Zlokovic, BT Lund, A Montagne, A Bonnin Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation Proceedings of the National Academy of Sciences of the United States of America, 2022-04-04;119(15):e2113310119. 2022-04-04 [PMID: 35377817] (IHC-F, Mouse) IHC-F Mouse
Show All 57 Publications.

Reviews for PDGF R beta Antibody (AF1042) (0)

There are no reviews for PDGF R beta Antibody (AF1042). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PDGF R beta Antibody (AF1042) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PDGF R beta Products

Diseases for PDGF R beta Antibody (AF1042)

Discover more about diseases related to PDGF R beta Antibody (AF1042).
 

Pathways for PDGF R beta Antibody (AF1042)

View related products by pathway.

PTMs for PDGF R beta Antibody (AF1042)

Learn more about PTMs related to PDGF R beta Antibody (AF1042).

Blogs on PDGF R beta

There are no specific blogs for PDGF R beta, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PDGF R beta Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PDGFRB
Uniprot