PDGF R beta Antibody [Unconjugated]


PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) ...read more
Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC

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PDGF R beta Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta
Accession # P05622
Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Read Publications using
AF1042 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta Antibody [Unconjugated]

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC
  • JTK12
  • PDGF R beta
  • PDGFR1
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide


The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGF R beta Antibody (AF1042)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF1042 Applications Species
L Cai, SV Kirchleitn, D Zhao, M Li, JC Tonn, R Glass, RE Kälin Glioblastoma Exhibits Inter-Individual Heterogeneity of TSPO and LAT1 Expression in Neoplastic and Parenchymal Cells Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963507] (IHC, Mouse) IHC Mouse
I Wojahn, TH Lüdtke, VM Christoffe, MO Trowe, A Kispert TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung Respir. Res., 2019;20(1):292. 2019 [PMID: 31870435] (IHC-P, Mouse) IHC-P Mouse
YK Lee, H Uchida, H Smith, A Ito, T Sanchez The isolation and molecular characterization of cerebral microvessels Nat Protoc, 2019;0(0):. 2019 [PMID: 31586162] (IHC, Mouse) IHC Mouse
DY Chen, NH Sun, YP Lu, LJ Hong, TT Cui, CK Wang, XH Chen, SS Wang, LL Feng, WX Shi, K Fukunaga, Z Chen, YM Lu, F Han GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury Theranostics, 2019;9(20):5937-5955. 2019 [PMID: 31534530] (ICC, Human) ICC Human
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019;10(1):2817. 2019 [PMID: 31249304] (IHC, Mouse) IHC Mouse
J Rose Dayto, MC Franke, Y Yuan, L Cruz-Oreng Straightforward method for singularized and region-specific CNS microvessels isolation J. Neurosci. Methods, 2019;0(0):. 2019 [PMID: 30797797] (IHC, Mouse) IHC Mouse
JK Lighthouse, RM Burke, LS Velasquez, RA Dirkx, A Aiezza, CS Moravec, JD Alexis, A Rosenberg, EM Small Exercise promotes a cardioprotective gene program in resident cardiac fibroblasts JCI Insight, 2019;4(1):. 2019 [PMID: 30626739] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Q Ma, Z Zhao, AP Sagare, Y Wu, M Wang, NC Owens, PB Verghese, J Herz, DM Holtzman, BV Zlokovic Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-?42 by LRP1-dependent apolipoprotein E isoform-specific mechanism Mol Neurodegener, 2018;13(1):57. 2018 [PMID: 30340601] (IHC, Mouse) IHC Mouse
T Liu, W Ma, H Xu, M Huang, D Zhang, Z He, L Zhang, S Brem, DM O'Rourke, Y Gong, Y Mou, Z Zhang, Y Fan PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma Nat Commun, 2018;9(1):3439. 2018 [PMID: 30150753] (Western Blot, Human) Western Blot Human
A Dubrac, SE Künzel, SH Künzel, J Li, RR Chandran, K Martin, DM Greif, RH Adams, A Eichmann NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy Nat Commun, 2018;9(1):3463. 2018 [PMID: 30150707] (IHC, Mouse) IHC Mouse
Show All 27 Publications.

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Biotin BAF1042

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Gene Symbol PDGFRB