PDGF R beta Antibody [Unconjugated]

Images

 
PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) ...read more
Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

PDGF R beta Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta
Leu32-Lys530
Accession # P05622
Specificity
Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRB
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1042 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta Antibody [Unconjugated]

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK12
  • PDGF R beta
  • PDGFR
  • PDGFR1
  • PDGFRB
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGF R beta Antibody (AF1042)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Mice.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
IHC
(24)
IHC-Fr
(4)
IHC-P
(6)
Neutralization
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(37)
Rat
(1)
Mice
(1)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using AF1042 Applications Species
L Cheng, M Zheng, J Jing, S Yu, Z Li, X Xu, F Yao, Y Luo, Y Liu M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury Neural regeneration research, 2021;0(0):. 2021 [PMID: 34916413] (IHC, Mouse) IHC Mouse
S Yu, L Cheng, D Tian, Z Li, F Yao, Y Luo, Y Liu, Z Zhu, M Zheng, J Jing Fascin-1 is Highly Expressed Specifically in Microglia After Spinal Cord Injury and Regulates Microglial Migration Frontiers in Pharmacology, 2021;12(0):729524. 2021 [PMID: 34646136] (IHC, Mouse) IHC Mouse
SS Yu, ZY Li, XZ Xu, F Yao, Y Luo, YC Liu, L Cheng, MG Zheng, JH Jing M1-type microglia can induce astrocytes to deposit chondroitin sulfate proteoglycan after spinal cord injury Neural regeneration research, 2022;17(5):1072-1079. 2022 [PMID: 34558535] (IHC, Mice) IHC Mice
JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation Frontiers in Cellular Neuroscience, 2021;15(0):683687. 2021 [PMID: 34557075] (IHC, Human) IHC Human
JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state Journal of hematology & oncology, 2021;14(1):148. 2021 [PMID: 34530889] (Flow Cytometry, Mouse) Flow Cytometry Mouse
L Wang, D Sievert, AE Clark, S Lee, H Federman, BD Gastfriend, EV Shusta, SP Palecek, AF Carlin, JG Gleeson A human three-dimensional neural-perivascular 'assembloid' promotes astrocytic development and enables modeling of SARS-CoV-2 neuropathology Nature Medicine, 2021;0(0):. 2021 [PMID: 34244682] (IHC, Human) IHC Human
Z Li, M Zheng, S Yu, F Yao, Y Luo, Y Liu, D Tian, L Cheng, J Jing M2 Macrophages Promote PDGFR&beta+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFR&beta Pathway Frontiers in Pharmacology, 2021;12(0):670813. 2021 [PMID: 33935795] (Western Blot, Mouse) Western Blot Mouse
K Sakai, F Takata, G Yamanaka, M Yasunaga, K Hashiguchi, K Tominaga, K Itoh, Y Kataoka, A Yamauchi, S Dohgu Reactive pericytes in early phase are involved in glial activation and late-onset hypersusceptibility to pilocarpine-induced seizures in traumatic brain injury model mice Journal of pharmacological sciences, 2021;145(1):155-165. 2021 [PMID: 33357774] (IHC, Mouse) IHC Mouse
TM O?Shea, AL Wollenberg, JH Kim, Y Ao, TJ Deming, MV Sofroniew Foreign body responses in mouse central nervous system mimic natural wound responses and alter biomaterial functions Nature Communications, 2020;11(1):6203. 2020 [PMID: 33277474] (IHC, Mouse) IHC Mouse
JL Kadrmas, MC Beckerle, M Yoshigi Genetic analyses in mouse fibroblast and melanoma cells demonstrate novel roles for PDGF-AB ligand and PDGF receptor alpha Sci Rep, 2020;10(1):19303. 2020 [PMID: 33168840] (Western Blot, Rat) Western Blot Rat
Show All 44 Publications.

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Bioinformatics

Gene Symbol PDGFRB
Uniprot