M-CSFR/CD115 Antibodies

HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND₅₀ is typically 4-16 ng/mL." class="big_lightbox"> 329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND₅₀ is typically 4-16 ng/mL." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " alt="THP-1 cells were stained with Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

329-MR) was serially diluted and captured by Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3292) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 329-MR) was serially diluted and captured by Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3292) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="THP-1 cells were stained with Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB329, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

329-MR) was serially diluted and captured by Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3292) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 329-MR) was serially diluted and captured by Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3292) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human M-CSF R/CD115 Fc Chimera (Catalog # 329-MR) was serially diluted and captured by Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3292) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M-CSF R/CD115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF329) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />