Recombinant Mouse M-CSF Protein


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Recombinant Mouse M-CSF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
29 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
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416-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930


M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF protein is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF protein can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML)(359)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.

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Showing Publications 1 - 10 of 359. Show All 359 Publications.
Publications using 416-ML Applications Species
T Velletri, Y Huang, Y Wang, Q Li, M Hu, N Xie, Q Yang, X Chen, Q Chen, P Shou, Y Gan, E Candi, AP Margherita, M Agostini, H Yang, G Melino, Y Shi, Y Wang Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin Cell Death Differ., 2020;0(0):. 2020 [PMID: 32694652] (Cell Culture, Mouse) Cell Culture Mouse
K Maruyama, H Kidoya, N Takemura, E Sugisawa, O Takeuchi, T Kondo, MMA Eid, H Tanaka, MM Martino, N Takakura, Y Takayama, S Akira, A Vandenbon, Y Kumagai Zinc Finger Protein St18 Protects against Septic Death by Inhibiting VEGF-A from Macrophages Cell Rep, 2020;32(2):107906. 2020 [PMID: 32668247] (Bioassay, Mouse) Bioassay Mouse
Y Tsuchiya, M Hayashi, K Nagamatsu, T Ono, M Kamakura, T Iwata, T Nakashima The key royal jelly component 10-hydroxy-2-decenoic acid protects against bone loss by inhibiting NF-&amp;kappaB signaling downstream of FFAR4 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32647011] (Cell Culture, Mouse) Cell Culture Mouse
M Chen, Y Zhang, P Zhou, X Liu, H Zhao, X Zhou, Q Gu, B Li, X Zhu, Q Shi Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-&amp;kappaB signaling pathway Bioact Mater, 2020;5(4):880-890. 2020 [PMID: 32637751] (Cell Culture, Mouse) Cell Culture Mouse
P Xu, C Shan, TJ Dunn, X Xie, H Xia, J Gao, J Allende La, J Zou, PP Villarreal, CR Schlagal, Y Yu, G Vargas, SL Rossi, N Vasilakis, PY Shi, SC Weaver, P Wu Role of microglia in the dissemination of Zika virus from mother to fetal brain PLoS Negl Trop Dis, 2020;14(7):e0008413. 2020 [PMID: 32628667] (Cell Culture, Mouse) Cell Culture Mouse
JH Kang, HM Ko, GD Han, SY Lee, JS Moon, MS Kim, JT Koh, SH Kim Dual role of phosphatidylserine and its receptors in osteoclastogenesis Cell Death Dis, 2020;11(7):497. 2020 [PMID: 32612123] (Cell Culture, Mouse) Cell Culture Mouse
KS Shim, YH Hwang, SA Jang, T Kim, H Ha Water Extract of Lysimachia christinae Inhibits Trabecular Bone Loss and Fat Accumulation in Ovariectomized Mice Nutrients, 2020;12(7):. 2020 [PMID: 32610585] (Cell Culture, Mouse) Cell Culture Mouse
H Chen, C Fang, X Zhi, S Song, Y Gu, X Chen, J Cui, Y Hu, W Weng, Q Zhou, Y Wang, Y Wang, H Jiang, X Li, L Cao, X Chen, J Su Neobavaisoflavone inhibits osteoclastogenesis through blocking RANKL signalling-mediated TRAF6 and c-Src recruitment and NF-&amp;kappaB, MAPK and Akt pathways J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32604472] (Cell Culture, Mouse) Cell Culture Mouse
Z Hu, N Deng, K Liu, N Zhou, Y Sun, W Zeng CNTF-STAT3-IL-6 Axis Mediates Neuroinflammatory Cascade across Schwann Cell-Neuron-Microglia Cell Rep, 2020;31(7):107657. 2020 [PMID: 32433966] (In Vivo, Mouse) In Vivo Mouse
P Saha, X Xiao, Y Li, RM Golonka, AA Abokor, BS Yeoh, M Vijay-Kuma Distinct iron homeostasis in C57BL/6 and Balb/c mouse strains Physiol Rep, 2020;8(9):e14441. 2020 [PMID: 32385968] (Bioassay, Mouse) Bioassay Mouse
Show All 359 Publications.

Reviews for M-CSF (416-ML) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 416-ML:
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reviewed by:
Walter Jackson
CellProlif 02/22/2021


ApplicationCell Proliferation
CommentsIt worked well in the proliferation and differentiation of mouse bone marrow cells in vitro.
 M-CSF 416-ML
reviewed by:


CommentsExcellent activity in cell culture. Effective in differentiating mouse bone marrow cells into BMDMs. Adhesion and cell health are very good for this timepoint. Price was also acceptable for the product, given comparables.


CommentsPreparing murine IFNAR-/- bone marrow macrophages for viral infection/entry studies.

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Walter Jackson
Application: CellProlif



Gene Symbol Csf1