Recombinant Mouse M-CSF Protein

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse M-CSF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5‑3 ng/mL.
Source
E. coli-derived mouse M-CSF protein
Lys33-Glu262, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Csf1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
416-ML in the following application:

Publications
Read Publications using
416-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse M-CSF Protein

  • colony stimulating factor 1 (macrophage)
  • CSF1
  • CSF-1
  • Lanimostim
  • macrophage colony stimulating factor
  • macrophage colony-stimulating factor 1
  • MCSF
  • M-CSF
  • MCSFlanimostim
  • MGC31930

Background

M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1-3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1-5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3-9). Full length mouse M-CSF transcripts encode a 520 amino acid (aa) type I transmembrane (TM) protein with a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M‑CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 229 aa of mature mouse M-CSF shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human M-CSF, respectively (12, 13). Human M‑CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

  1. Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628.
  2. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
  3. Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125.
  4. Ryan, G.R. et al. (2001) Blood 98:74.
  5. Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178.
  6. Nandi, S. et al. (2006) Blood 107:786.
  7. Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026.
  8. Suzu, S. et al. (1992) J. Biol. Chem. 267:16812.
  9. Manos, M.M. (1988) Mol. Cell. Biol. 8:5035.
  10. Koths, K. (1997) Mol. Reprod. Dev. 46:31.
  11. Jang, M-H. et al. (2006) J. Immunol. 177:4055.
  12. DeLamarter, J.F. et al. (1987) Nucleic Acids Res. 15:2389.
  13. Ladner, M.B. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6706.

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Publications for M-CSF (416-ML)(327)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Feline.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.


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Bioassay
(303)
Cell Culture
(13)
Differentiation
(3)
In Vivo
(1)
Tissue Culture
(1)
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Human
(2)
Mouse
(318)
Rat
(1)
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(1)
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Showing Publications 1 - 10 of 327. Show All 327 Publications.
Publications using 416-ML Applications Species
AL Rocha, RL Bighetti-T, LF Duffles, JAA de Arruda, TM Taira, BRD Assis, S Macari, IMA Diniz, MM Beloti, AL Rosa, SY Fukada, GAC Goulart, DD Ribeiro, LG Abreu, TA Silva Inhibitory effects of dabigatran etexilate, a direct thrombin inhibitor, on osteoclasts and osteoblasts Thromb. Res., 2019;186(0):45-53. 2019 [PMID: 31883999] (Bioassay, Mouse) Bioassay Mouse
J Hu, X Li, Y Chen, X Han, L Li, Z Yang, L Duan, H Lu, Q He The protective effect of WKYMVm peptide on inflammatory osteolysis through regulating NF-kappaB and CD9/gp130/STAT3 signalling pathway J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 31837208] (Bioassay, Mouse) Bioassay Mouse
AE Ciecko, B Foda, JY Barr, S Ramanathan, MA Atkinson, DV Serreze, AM Geurts, SM Lieberman, YG Chen Interleukin-27 Is Essential for Type 1 Diabetes Development and Sj�gren Syndrome-like Inflammation Cell Rep, 2019;29(10):3073-3086.e5. 2019 [PMID: 31801074] (Tissue Culture, Mouse) Tissue Culture Mouse
D Huang, X Luo, Z Yin, J Xu, Q Gu Diterpenoids from the aerial parts of Flueggea acicularis and their activity against RANKL-induced osteoclastogenesis Bioorg. Chem., 2019;0(0):103453. 2019 [PMID: 31787342] (Bioassay, Mouse) Bioassay Mouse
SK Pore, ER Hahm, SH Kim, KB Singh, L Nyiranshut, JD Latoche, CJ Anderson, J Adamik, DL Galson, KR Weiss, RJ Watters, B Lee, PN Kumta, SV Singh A Novel Sulforaphane-Regulated Gene Network in Suppression of Breast Cancer-Induced Osteolytic Bone Resorption Mol. Cancer Ther., 2019;0(0):. 2019 [PMID: 31784454] (Bioassay, Mouse) Bioassay Mouse
J Wang, G Chen, Q Zhang, F Zhao, X Yu, X Ma, M Liu Phillyrin Attenuates Osteoclast Formation and Function and Prevents LPS-Induced Osteolysis in Mice Front Pharmacol, 2019;10(0):1188. 2019 [PMID: 31680965] (Bioassay, Mouse) Bioassay Mouse
J Nagai, B Balestrier, LB Fanning, T Kyin, H Cirka, J Lin, M Idzko, A Zech, EY Kim, PJ Brennan, JA Boyce P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation J. Clin. Invest., 2019;0(0):. 2019 [PMID: 31638598] (Bioassay, Mouse) Bioassay Mouse
RW Wu, WS Lian, YS Chen, CW Kuo, HC Ke, CK Hsieh, SY Wang, JY Ko, FS Wang MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis Int J Mol Sci, 2019;20(20):. 2019 [PMID: 31627291] (Cell Culture, Mouse) Cell Culture Mouse
S Avasarala, PY Wu, SQ Khan, S Yanlin, M Van Scoyk, J Bao, A Di Lorenzo, O David, MT Bedford, V Gupta, RA Winn, RK Bikkavilli PRMT6 promotes lung tumor progression via the alternate activation of tumor-associated macrophages Mol. Cancer Res., 2019;0(0):. 2019 [PMID: 31619507] (Cell Culture, Mouse) Cell Culture Mouse
N Wu, XN Nguyen, L Wang, R Appourchau, C Zhang, B Panthu, H Gruffat, C Journo, S Alais, J Qin, N Zhang, K Tartour, F Catez, R Mahieux, T Ohlmann, M Liu, B Du, A Cimarelli The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation PLoS Pathog., 2019;15(10):e1008093. 2019 [PMID: 31600344] (Bioassay, Mouse) Bioassay Mouse
Show All 327 Publications.

Review for M-CSF (416-ML) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 416-ML:
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 M-CSF 416-ML
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reviewed by:
Anonymous
09/09/2019
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Summary

LotME4619021
CommentsExcellent activity in cell culture. Effective in differentiating mouse bone marrow cells into BMDMs. Adhesion and cell health are very good for this timepoint. Price was also acceptable for the product, given comparables.

Comments

CommentsPreparing murine IFNAR-/- bone marrow macrophages for viral infection/entry studies.

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Anonymous
09/09/2019
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Gene Symbol Csf1
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