Lysyl Oxidase Homolog 2/LOXL2 Antibody

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Genetic Strategies: Knockout Validated: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane ...read more
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LOXL2 stained by LOXL2 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.LOXL2 ...read more
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 ...read more
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at a dilution of 1:1000.
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody ) diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Paraffin-embedded human cervical carcinoma diluted at 1:500.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Paraffin-embedded mouse kidney. LOXL2 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian carcinoma. LOXL2 antibody at 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.

Product Details

Summary
Reactivity Hu, Mu, Fe, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Lysyl Oxidase Homolog 2/LOXL2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.
Localization
Secreted, extracellular space
Predicted Species
Rat (91%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOXL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Lysyl Oxidase Homolog 2/LOXL2 Knockout HeLa Cell Lysate
Lysyl Oxidase Homolog 2/LOXL2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP1-32954 in the following applications:

Publications
Read Publications using
NBP1-32954 in the following applications:

  • 2 publications
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (83%). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Lysyl Oxidase Homolog 2/LOXL2 Antibody

  • EC 1.4.3
  • EC 1.4.3.-
  • LOL2
  • LOR2
  • LOXL2
  • Lysyl Oxidase Homolog 2
  • lysyl oxidase related 2
  • lysyl oxidase-like 2
  • Lysyl oxidase-like protein 2
  • Lysyl oxidase-related protein 2
  • Lysyl oxidase-related protein WS9-14
  • WS9-14

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, KO

Publications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC-P, KD, WB.


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IHC-P
(2)
KD
(1)
WB
(4)
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Human
(5)
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Review for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: cat.

Reviews using NBP1-32954:
Filter by Applications
ICC
(1)
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Filter by Species
cat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Lysyl Oxidase Homolog 2/LOXL2 NBP1-32954
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reviewed by:
Anonymous
ICC cat 01/14/2019
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Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
Speciescat
Lot42389

Comments

CommentsFeline cardiac fibroblasts were blocked in 100µl of 10% Goat serum for 30 min and 0.85µl of LOXL2 antibody was added in each well in the 24-well plate. The cells were incubated with the primary antibody at 4C overnight, then were washed 3 times with PBST (5 min each). Goat anti rabbit IgG conjugated with Cy2 were used as secondary. DAPI was also added in for staining of the nuclei.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954). (Showing 1 - 1 of 1 FAQs).

  1. What is the appropriate positive control for NBP1-32954 in immunohistochemistry on paraffin embedded sections?
    • According to The Human Protein Atlas Lysyl oxidase homolog 2 protein is expressed in most tissues. You can use any of the tissues with good (at least medium) protein expression levels as your positive control for IHC. Please see the graph and examples of IHC tissue stainings here: https://www.proteinatlas.org/ENSG00000134013-LOXL2/tissue. For example, I see the references for this product successfully stained human lung tissue, ovarian and breast carcinomas. If you wish to purchase a tissue slide to be used as a control, we have some in our catalog. You can find them here: https://www.novusbio.com/search?keywords=tissue%20normal&category=Slides&applications=Immunohistochemistry-Paraffin and https://www.novusbio.com/search?keywords=tissue%20normal&category=Tissue%20Microarrays

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
01/14/2019
Application: ICC
Species: cat

Bioinformatics

Gene Symbol LOXL2
Entrez
Uniprot