Lysyl Oxidase Homolog 2/LOXL2 Antibody

Images

 
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody diluted at 1:1000. The ...read more
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LOXL2 stained by LOXL2 antibody diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human ovarian cancer. LOXL2 stained by LOXL2 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 ...read more
Immunocytochemistry/ Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody ) diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Human esophageal carcinoma.LOXL2 stained by LOXL2 antibody diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Collagen organization and lysyl oxidase family of enzymes are differentially expressed andinterrelatedifferently in IPF. ...read more
Knockout Validated: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 ...read more
ELISA: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP1-32954] - Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (44.94-0.7 nM). Coated protein was probed with LOXL2 ...read more

Product Details

Summary
Reactivity Hu, Mu, Fe, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Lysyl Oxidase Homolog 2/LOXL2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.
Localization
Secreted, extracellular space
Predicted Species
Rat (91%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOXL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:10000
Application Notes
Elisa-Assay dependent
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-32954 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • KD
    1 publication
  • WB
    4 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (87%), Xenopus laevis (83%). Feline reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Lysyl Oxidase Homolog 2/LOXL2 Antibody

  • EC 1.4.3
  • EC 1.4.3.-
  • LOL2
  • LOR2
  • LOXL2
  • Lysyl Oxidase Homolog 2
  • lysyl oxidase related 2
  • lysyl oxidase-like 2
  • Lysyl oxidase-like protein 2
  • Lysyl oxidase-related protein 2
  • Lysyl oxidase-related protein WS9-14
  • WS9-14

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-32954
Species: Hu, Mu, Fe, Rt, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, KO

Publications for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-P, KD, WB.


Filter By Application
IHC
(1)
IHC-P
(2)
KD
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-32954 Applications Species
Yamamoto A, Saito T, Hosoya T et al. Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis Arthritis & rheumatology (Hoboken, N.J.) Dec 9 2021 [PMID: 34882985]
Cebria-Costa JP, Pascual-Reguant L, Gonzalez-Perez A et al. LOXL2-mediated H3K4 oxidation reduces chromatin accessibility in triple-negative breast cancer cells Oncogene Aug 28 2019 [PMID: 31462706] (WB, Human) WB Human
Jeong YJ, Park SH, Mun SH et al. Association between lysyl oxidase and fibrotic focus in relation with inflammation in breast cancer Oncology Letters [PMID: 29434955] (IHC-P, Human) IHC-P Human
Tjin G, White ES, Faiz A et al. Lysyl oxidases regulate fibrillar collagen remodelling in idiopathic pulmonary fibrosis. Dis Model Mech 2017 Nov 01 [PMID: 29125826] (IHC-P, Human) IHC-P Human
Sato F, Seino-Sudo R, Okada M et al. Lysyl Oxidase Enhances the Deposition of Tropoelastin through the Catalysis of Tropoelastin Molecules on the Cell Surface. Biol. Pharm. Bull. 2017 Oct 02 [PMID: 28966236] (WB) WB
Wong CC, Zhang H, Gilkes DM et al. Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis. J Mol Med (Berl) 2012 Jul [PMID: 22231744] (WB, Human) WB Human
Querol J Maintaining oxidized H3 in heterochromatin is required for the oncogenic capacity of triple-negative breast cancer cells bioRxiv Jan 1 2020 (WB, KD, Human) WB, KD Human
Torres S, Garcia-Palmero I, et al. LOXL2 Is Highly Expressed in Cancer-Associated Fibroblasts and Associates to Poor Colon Cancer Survival. Clin Cancer Res 2015-11-01 [PMID: 26206869] (IHC, Human) IHC Human

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysyl Oxidase Homolog 2/LOXL2 Antibody (NBP1-32954). (Showing 1 - 1 of 1 FAQs).

  1. What is the appropriate positive control for NBP1-32954 in immunohistochemistry on paraffin embedded sections?
    • According to The Human Protein Atlas Lysyl oxidase homolog 2 protein is expressed in most tissues. You can use any of the tissues with good (at least medium) protein expression levels as your positive control for IHC. Please see the graph and examples of IHC tissue stainings here: https://www.proteinatlas.org/ENSG00000134013-LOXL2/tissue. For example, I see the references for this product successfully stained human lung tissue, ovarian and breast carcinomas. If you wish to purchase a tissue slide to be used as a control, we have some in our catalog. You can find them here: https://www.novusbio.com/search?keywords=tissue%20normal&category=Slides&applications=Immunohistochemistry-Paraffin and https://www.novusbio.com/search?keywords=tissue%20normal&category=Tissue%20Microarrays

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Bioinformatics

Gene Symbol LOXL2
Entrez
Uniprot