Snail Antibody [Unconjugated]

Western blot shows lysates of A549 human lung carcinoma cell line and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Snail at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-Cadherin promoter was detected by standard PCR.

Snail was detected in immersion fixed A549 human lung carcinoma cell line treated with Recombinant Human TGF-beta 1 (left panel, Catalog # 240-B) or untreated (right panel) using Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

SerpinA1 was regulated by SnailA. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), and Snail and serpinA1 protein levels were evaluated by western blot analysis. B. DLD-1 and SW480 cells were transfected with pcDNA-serpinA1 (serpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), and western blot analysis was performed for detection of Snail and SerpinA1 expression. C. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail) or control vector pcDNA (vector), and ChIP assays were performed. The presence of the serpinA1 promoter (−516/−4) was verified in immunoprecipitates with either mouse IgG or anti-Snail antibodies, and assay inputs were analyzed using real-time PCR. The samples were loaded on agarose gels. D. Data show promoter enrichment in the anti-Snail immunoprecipitate relative to IgG. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by R&D Systems.

SerpinA1 was regulated by SnailA. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), and Snail and serpinA1 protein levels were evaluated by western blot analysis. B. DLD-1 and SW480 cells were transfected with pcDNA-serpinA1 (serpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), and western blot analysis was performed for detection of Snail and SerpinA1 expression. C. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail) or control vector pcDNA (vector), and ChIP assays were performed. The presence of the serpinA1 promoter (−516/−4) was verified in immunoprecipitates with either mouse IgG or anti-Snail antibodies, and assay inputs were analyzed using real-time PCR. The samples were loaded on agarose gels. D. Data show promoter enrichment in the anti-Snail immunoprecipitate relative to IgG. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Snail and serpinA1 promoted tumor progression through fibronectinA. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. B. DLD-1 and SW480 cells were transfected with pcDNA-serpinA1 (SerpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. C. DLD-1 and SW480 cells were transfected with pcDNA-fibronectin (Fibronectin), control vector pcDNA (vector), fibronectin siRNA (siFibronectin), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. D., E. Invasion and migration assays were performed using transfected cells. Representative data are shown for cells that invaded (top) and migrated (bottom) in the presence of 1% FBS. *P < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Snail and serpinA1 promoted tumor progression through fibronectinA. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. B. DLD-1 and SW480 cells were transfected with pcDNA-serpinA1 (SerpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. C. DLD-1 and SW480 cells were transfected with pcDNA-fibronectin (Fibronectin), control vector pcDNA (vector), fibronectin siRNA (siFibronectin), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. D., E. Invasion and migration assays were performed using transfected cells. Representative data are shown for cells that invaded (top) and migrated (bottom) in the presence of 1% FBS. *P < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Snail and serpinA1 promoted tumor progression through fibronectinA. DLD-1 and SW480 cells were transfected with pcDNA-Snail (Snail), control vector pcDNA (vector), Snail siRNA (siSnail), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. B. DLD-1 and SW480 cells were transfected with pcDNA-serpinA1 (SerpinA1), control vector pcDNA (vector), serpinA1 siRNA (siSerpinA1), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. C. DLD-1 and SW480 cells were transfected with pcDNA-fibronectin (Fibronectin), control vector pcDNA (vector), fibronectin siRNA (siFibronectin), or nontargeting siRNA (siNT), and Snail, serpinA1, and fibronectin protein levels were evaluated by western blot analysis. D., E. Invasion and migration assays were performed using transfected cells. Representative data are shown for cells that invaded (top) and migrated (bottom) in the presence of 1% FBS. *P < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26015410), licensed under a CC-BY license. Not internally tested by R&D Systems.

PDGF-AB induces Endo-MT through NF-kB-mediated Snail expression in ECs. a Human brain microvacular ECs were treated with glioma-CM. RNA was isolated and subjected to RNA-seq analysis. Left, heat map for expression of EMT-related transcriptional factors. Right, fold change of these transcriptional factors (n = 3, mean ± SEM). b, c ECs were treated with 100 ng/ml PDGF-AA, PDGF-AB, and PDGF-BB. b Cell lysates were immunoblotted. c RNA was isolated and analyzed by RT-PCR. Results were normalized with GAPDH levels (n = 3, mean ± SEM). d ECs were transfected with siRNAs targeting Snail or control scrambled sequence, and treated with PDGF-AB. Cell lysates were immunoblotted. e ECs were transfected with siRNAs targeting Erg-1, NF-kappa B, or control scrambled sequence, and treated with PDGF-AB. Cell lysates were immunoblotted. f ECs were treated with PDGF-AB for 2 h. Cells were analyzed by immunofluorescence. g, h ECs were treated with PDGF-AB or control medium for 8 h. Nuclear extracts were immunoprecipitated with anti-NF-kappa B antibody or IgG, and subjected to ChIP analysis with primers #1 and #2. g DNA was resolved by agarose electrophoresis, and imaged. Shown are representative results with primer #2. The arrow indicates the amplified DNA in Snail promoter. h Quantitative PCR analysis (n = 3, mean ± SEM). i ECs were transfected with siRNAs targeting Snail or control scrambled sequence, 4 days after treatment with Ki8751, followed by cell viability analysis (n = 3, mean ± SD) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30150753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative images of positive IHC staining in benign and malignant prostate tissue and a negative control Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28877722), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ChIP, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Snail Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Snail
  Pro2-Arg264
  Accession # O95863
• Specificity: Detects human Snail in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNAI1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Snail Antibody [Unconjugated]

• dJ710H13.1
• Protein sna
• Protein snail homolog 1
• SLUGH2
• SNA
• SNAH
• SNAI1
• snail 1 homolog
• snail 1 zinc finger protein
• snail family zinc finger 1
• snail homolog 1
• Snail
• SNAIL1
• zinc finger protein SNAI1

Background

Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Snail Antibody (AF3639)(37)

Publications using AF3639

• Setyono, ESA;Rogers, NK;Hofmann, A;Lickert, H;Burtscher, I; Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation Stem cell research 2025-02-15 [PMID: 40022904] (Immunocytochemistry, Human)
• Despin-Guitard, E;Rosa, VS;Plunder, S;Mathiah, N;Van Schoor, K;Nehme, E;Merino-Aceituno, S;Egea, J;Shahbazi, MN;Theveneau, E;Migeotte, I; Non-apical mitoses contribute to cell delamination during mouse gastrulation Nature communications 2024-08-28 [PMID: 39198421] (Immunohistochemistry, Mouse)
• Rokavec, M;Jaeckel, S;Hermeking, H; Nidogen-1/NID1 Function and Regulation during Progression and Metastasis of Colorectal Cancer Cancers 2023-11-07 [PMID: 38001576] (Chromatin Immunoprecipitation (ChIP), Human)
• JD Twomey, B Zhang Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer Biomedicines, 2023-03-17;11(3):. 2023-03-17 [PMID: 36979915] (Western Blot, Human)
• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
• M Simunovic, ED Siggia, AH Brivanlou In�vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells Cell Stem Cell, 2022-06-02;29(6):962-972.e4. 2022-06-02 [PMID: 35659878] (IHC, Human)
• Menggui Huang, Fan Yang, Duo Zhang, Maohuan Lin, Hao Duan, Rakan El-Mayta et al. Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction Nature Cardiovascular Research 5/16/2022 [PMID: 35571674]
• Sophie M Morgani, Jie Su, Jennifer Nichols, Joan Massagué, Anna-Katerina Hadjantonakis The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos eLife 4/30/2021 [PMID: 33929320]
• Liqun Li, Qiang Liu, Tongyao Shang, Wei Song, Dongmei Xu, Thaddeus D. Allen et al. Aberrant Activation of Notch1 Signaling in Glomerular Endothelium Induces Albuminuria Circulation Research 3/5/2021 [PMID: 33435713]
• Tian X, Yu H, Li D et al. The miR-5694/AF9/Snail Axis Provides Metastatic Advantages and a Therapeutic Target in Basal-like Breast Cancer Molecular Therapy 3/1/2021 [PMID: 33221433]
• Tian X, Yu H, Li D et al. The miR-5694/AF9/Snail Axis Provides Metastatic Advantages and a Therapeutic Target in Basal-like Breast Cancer Molecular Therapy 2021-03-01 [PMID: 33221433]
• Battistelli C, Garbo S, Riccioni V et al. Design and Functional Validation of a Mutant Variant of the LncRNA HOTAIR to Counteract Snail Function in Epithelial-to-Mesenchymal Transition Cancer Research 1/1/2021 [PMID: 33158813]
• Battistelli C, Garbo S, Riccioni V et al. Design and Functional Validation of a Mutant Variant of the LncRNA HOTAIR to Counteract Snail Function in Epithelial-to-Mesenchymal Transition Cancer Research 2021-01-01 [PMID: 33158813]
• Emma D. Zanfi, Sebastian Fantini, Roberta Lotti, Matteo Bertesi, Alessandra Marconi, Alexis Grande et al. Wnt/CTNNB1 Signal Transduction Pathway Inhibits the Expression of ZFP36 in Squamous Cell Carcinoma, by Inducing Transcriptional Repressors SNAI1, SLUG and TWIST International Journal of Molecular Sciences 8/8/2020 [PMID: 32784485]
• Klaudia Skrzypek, Marta Kot, Paweł Konieczny, Artur Nieszporek, Anna Kusienicka, Małgorzata Lasota et al. SNAIL Promotes Metastatic Behavior of Rhabdomyosarcoma by Increasing EZRIN and AKT Expression and Regulating MicroRNA Networks Cancers (Basel) 7/11/2020 [PMID: 32664538]
• NC Boles, M Fernandes, T Swigut, R Srinivasan, L Schiff, A Rada-Igles, Q Wang, JS Saini, T Kiehl, JH Stern, J Wysocka, TA Blenkinsop, S Temple Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide Stem Cell Reports, 2020-04-02;14(4):631-647. 2020-04-02 [PMID: 32243845] (ICC, Human)
• Sofiane Hamidi, Yukiko Nakaya, Hiroki Nagai, Cantas Alev, Takeya Kasukawa, Sapna Chhabra et al. Mesenchymal-epithelial transition regulates initiation of pluripotency exit before gastrulation Development 2/1/2020 [PMID: 32014865]
• R Zhang, J Liu, W Zhang, L Hua, LT Qian, SB Zhou EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/beta-catenin pathway Cancer Cell Int., 2020-01-13;20(0):20. 2020-01-13 [PMID: 31956298] (IF, Human)
• K Fukuura, Y Inoue, C Miyajima, S Watanabe, M Tokugawa, D Morishita, N Ohoka, M Komada, H Hayashi The ubiquitin-specific protease USP17 prevents cellular senescence by stabilizing the methyltransferase SET8 and transcriptionally repressing p21 J. Biol. Chem., 2019-09-18;0(0):. 2019-09-18 [PMID: 31533987] (Western Blot, Human)
• G Rademaker, B Costanza, S Anania, F Agirman, N Maloujahmo, E Di Valenti, JJ Goval, A Bellahcène, V Castronovo, O Peulen Myoferlin Contributes to the Metastatic Phenotype of Pancreatic Cancer Cells by Enhancing Their Migratory Capacity through the Control of Oxidative Phosphorylation Cancers (Basel), 2019-06-19;11(6):. 2019-06-19 [PMID: 31248212] (Western Blot, Human)
• JM Oh, J Lee, WT Im, S Chun Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases Int J Mol Sci, 2019-03-11;20(5):. 2019-03-11 [PMID: 30862004] (Western Blot, Human)
• M Kurihara-S, T Sasahira, H Shimomura, C Nakashima, T Kirita The Oncogenic Activity of miR-29b-1-5p Induces the Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma J Clin Med, 2019-02-24;8(2):. 2019-02-24 [PMID: 30813466] (Western Blot, Human)
• T Liu, W Ma, H Xu, M Huang, D Zhang, Z He, L Zhang, S Brem, DM O'Rourke, Y Gong, Y Mou, Z Zhang, Y Fan PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma Nat Commun, 2018-08-27;9(1):3439. 8/27/2018 [PMID: 30150753]
• Sophie M Morgani, Jakob J Metzger, Jennifer Nichols, Eric D Siggia, Anna-Katerina Hadjantonakis Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning eLife 2/7/2018 [PMID: 29412136]
• Shyam Kumar Gudey, Reshma Sundar, Carl-Henrik Heldin, Anders Bergh, Marene Landström Pro-invasive properties of Snail1 are regulated by sumoylation in response to TGF? stimulation in cancer. Oncotarget, 2017-08-09;0(0):1949-2553. 2017-08-09 [PMID: 29228645] (Immunoprecipitation, Human, Mouse)
• Gonnissen A, Isebaert S, Perneel C et al. Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer BMC Cancer 9/6/2017 [PMID: 28877722]
• Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells. British Journal of Cancer, 2017-08-17;0(0):1532-1827. 2017-08-17 [PMID: 28817834] (Western Blot, Human)
• M Rokavec, M Kaller, D Horst, H Hermeking Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression Sci Rep, 2017-07-05;7(1):4687. 2017-07-05 [PMID: 28680090] (ChIP, Human)
• Anitha K. Shenoy, Yue Jin, Huacheng Luo, Ming Tang, Christine Pampo, Rong Shao et al. Epithelial-to-mesenchymal transition confers pericyte properties on cancer cells Journal of Clinical Investigation 10/10/2016 [PMID: 27721239]
• Clare Verrill, Lucia Cerundolo, Chad Mckee, Michael White, Christiana Kartsonaki, Eve Fryer et al. Altered expression of epithelial-to-mesenchymal transition proteins in extraprostatic prostate cancer Oncotarget 12/19/2015 [PMID: 26701730]
• Sabine Hünten, Heiko Hermeking p53 directly activates cystatin D/CST5 to mediate mesenchymal-epithelial transition: a possible link to tumor suppression by vitamin D3 Oncotarget 6/30/2015 [PMID: 26158294]
• Kwon CH, Park HJ, Choi JH et al. Snail and serpinA1 promote tumor progression and predict prognosis in colorectal cancer. Oncotarget 8/21/2015 [PMID: 26015410]
• Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression. Clin Cancer Res, 2014-12-16;21(4):899-906. 2014-12-16 [PMID: 25516888] (ICC, Human)
• Kwon CH, Park HJ, Lee JR et al. Serpin peptidase inhibitor clade A member 1 is a biomarker of poor prognosis in gastric cancer. Br J Cancer 11/11/2014 [PMID: 25211665]
• Sarah Javaid, Jianmin Zhang, Endre Anderssen, Josh C. Black, Ben S. Wittner, Ken Tajima et al. Dynamic Chromatin Modification Sustains Epithelial-Mesenchymal Transition following Inducible Expression of Snail-1 Cell Reports 12/26/2013 [PMID: 24360956]
• Sarah Javaid, Jianmin Zhang, Endre Anderssen, Josh C. Black, Ben S. Wittner, Ken Tajima et al. Dynamic Chromatin Modification Sustains Epithelial-Mesenchymal Transition following Inducible Expression of Snail-1 Cell Reports 2013-12-26 [PMID: 24360956] (Chromatin Immunoprecipitation (ChIP), Human)
• Schwock J, Bradley G, Ho JC SNAI1 expression and the mesenchymal phenotype: an immunohistochemical study performed on 46 cases of oral squamous cell carcinoma. BMC Clin Pathol, 2010-02-05;10(0):1. 2010-02-05 [PMID: 20181105] (IHC-P, Western Blot, Human)

FAQs for Snail Antibody (AF3639). (Showing 1 - 2 of 2 FAQs).

1. There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.
   • Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.
2. Can snail antibody AF3639 be used for electrophoresis motility shift assays (EMSA) instead of ChIP?
   • For IP/Co-IP and ChIP the antibodies need to provide sufficient binding affinity for the antibodies to stay bound to the protein targets during the precipitation steps, whereas in EMSA the antibodies will only need to bind to the protein targets during the migration through the weak electrophoretic forces. Almost all, if not all, antibodies that bind to their protein targets with reasonable affinity and do not fall off from their protein targets when bound to the targeted, labeled DNA/RNA molecules during PAGE will be rendered in the upper shift in EMSA, provided the binding doesn't block the interaction between the protein targets and the nucleic acids .

Bioinformatics

• Gene Symbol: SNAI1
• Uniprot:
  • O95863()