LOXL1 Antibody - Azide and BSA Free

Western Blot: LOXL1 Antibody [H00004016-D01P] - Analysis of LOXL1 expression in transfected 293T cell line by LOXL1 polyclonal antibody.Lane 1: LOXL1 transfected lysate(63.10 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: LOXL1 Antibody [H00004016-D01P] - Analysis of antibody to LOXL1 on HeLa cell. Antibody concentration 10 ug/ml.

Simple Western: LOXL1 Antibody [H00004016-D01P] - Simple Western lane views show lysates of human Hep3B cell line. Specific bands were detected for LOXL1 using LOXL1 antibody at 1:50 and 1:10 diltutions. This experiment was conducted using the 12-230 kDa separation system. Image from verified customer review.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LOXL1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: LOXL1 (NP_005567.2, 1 a.a. - 574 a.a.) full-length human protein. MALARGSRQLGALVWGACLCVLVHGQQAQPGQGSDPARWRQLIQWENNGQVYSLLNSGSEYVPAGPQRSESSSRVLLAGAPQAQQRRSHGSPRRRQAPSLPLPGRVGSDTVRGQARHPFGFGQVPDNWREVAVGDSTGMARARTSVSQQRHGGSASSVSASAFASTYRQQPSYPQQFPYPQAPFVSQYENYDPASRTYDQGFVYYRPAGGGVGAGAAAVASAGVIYPYQPRARYEEYGGGEELPEYPPQGFYPAPERPYVPPPPPPPDGLDRRYSHSLYSEGTPGFEQAYPDPGPEAAQAHGGDPRLGWYPPYANPPPEAYGPPRALEPPYLPVRSSDTPPPGGERNGAQQGRLSVGSVYRPNQNGRGLPDLVPDPNYVQASTYVQRAHLYSLRCAAEEKCLASTAYAPEATDYDVRVLLRFPQRVKNQGTADFLPNRPRHTWEWHSCHQHYHSMDEFSHYDLLDAATGKKVAEGHKASFCLEDSTCDFGNLKRYACTSHTQGLSPGCYDTYNADIDCQWIDITDVQPGNYILKVHVNPKYIVLESDFTNNVVRCNIHYTGRYVSATNCKIVQS
• Specificity: LOXL1 - lysyl oxidase-like 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOXL1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Simple Western
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. Use in IHC reported in secitific publication PMID: 32424143. LOXL1 Antibody validated for Simple Western from a verified customer review.

Reactivity Notes

Use in Mouse reported in secitific publication PMID: 32424143

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LOXL1 Antibody - Azide and BSA Free

• EC 1.4.3.13
• LOLEC 1.4.3
• LOXL
• LOXL1
• LOXLEC 1.4.3.-
• Lysyl Oxidase Homolog 1
• Lysyl Oxidase like 1
• lysyl oxidase-like 1
• Lysyl oxidase-like protein 1

Background

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LOXL1 Antibody (H00004016-D01P)(6)

Publications using H00004016-D01P

• Jiang C, Wang M, Yao W et al. Comprehensive Analysis on Prognosis and Immune Infiltration of Lysyl Oxidase Family Members in Pancreatic Adenocarcinoma With Experimental Verification Frontiers in Molecular Biosciences 2022-04-01 [PMID: 35433829]
• De Maria A, Zientek KD, David LL et al. Compositional Analysis of Extracellular Aggregates in the Eyes of Patients With Exfoliation Syndrome and Exfoliation Glaucoma Investigative Opthalmology & Visual Science 2021-12-29 [PMID: 34964803]
• Qingjiang H, Takaaki M, Shotaro K et al. Potential association of LOXL1 with peritoneal dissemination in gastric cancer possibly via promotion of EMT. PLoS One. 2020-10-23 [PMID: 33095806]
• Yu H, Ding J, Zhu H et al. LOXL1 confers antiapoptosis and promotes gliomagenesis through stabilizing BAG2 Cell Death Differ. 2020-05-18 [PMID: 32424143] (IF/IHC, WB, Human, Mouse)
• Sethi A, Wordinger RJ, Clark AF. Gremlin utilizes canonical and non-canonical TGFbeta signaling to induce lysyl oxidase (LOX) genes in human trabecular meshwork cells. Exp Eye Res. 2013-08-01 [PMID: 23748100] (WB, Human)
• Sethi A, Mao W, Wordinger RJ, Clark AF. Transforming Growth Factor Beta Induces Extracellular Matrix Protein Crosslinking Lysyl Oxidase (LOX) Genes in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci. 2011-05-05 [PMID: 21546528]

Review for LOXL1 Antibody (H00004016-D01P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Leah Perkins Simple Western Human 12/06/2022

Summary

• Application: Simple Western
• Sample Tested: hep3b cell line
• Species: Human
• Lot: N/A

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Test Ab: LOX-L1 Ab Cat#H00004016-D01P, NOVUS Sample: Hep3B cell line Simple protein (Jess): 12-230 kda seperation modules. Best Protein Lysate concentration: 1mg/ml Best Primary Ab dilution: 1:10 Secondary - Rabbit HRP

Bioinformatics

• Gene Symbol: LOXL1
• Entrez:
  • 4016(Human)
• Uniprot:
  • NP_005567.2(Human)