HNF-3 alpha/FoxA1 Antibody (3B3NB)

Images

 
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis of FOXA1 (3B3NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - FOXA1 (3B3NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin and ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis of FoxA1 in human prostate.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis using the Azide Free version of NBP2-45354. Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with ...read more
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - (1) Whole cell protein from PC3 and (2) MCF7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis of FoxA1 in human breast adenocarcinoma.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) [NBP2-45354] - Analysis using the Azide Free version of NBP2-45354. Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3B3NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HNF-3 alpha/FoxA1 Antibody (3B3NB) Summary

Immunogen
Partial recombinant human FOXA1 protein (between amino acids 350-473) [UniProt# P55317].
Predicted Species
Mouse (95%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Flow Cytometry 25 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:25 - 1:50
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Control
HNF-3 alpha/FoxA1 Knockout HeLa Cell Lysate
Publications
Read Publication using NBP2-45354.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (78%) and Xenopus (73%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (3B3NB)

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FoxA1 (forkhead box A1), also called HNF-3a (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 alpha/FoxA1 Antibody (NBP2-45354)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HNF-3 alpha/FoxA1 Antibody (NBP2-45354) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-45354AF350
Alexa Fluor 405 NBP2-45354AF405
Alexa Fluor 488 NBP2-45354AF488
Alexa Fluor 532 NBP2-45354AF532
Alexa Fluor 594 NBP2-45354AF594
Alexa Fluor 647 NBP2-45354AF647
Alexa Fluor 700 NBP2-45354AF700
Alexa Fluor 750 NBP2-45354AF750
Allophycocyanin NBP2-45354APC
Biotin NBP2-45354B
DyLight 350 NBP2-45354UV
DyLight 405 NBP2-45354V
DyLight 488 NBP2-45354G
DyLight 550 NBP2-45354R
DyLight 594 NBP2-45354DL594
DyLight 650 NBP2-45354C
DyLight 680 NBP2-45354FR
DyLight 755 NBP2-45354IR
FITC NBP2-45354F
HRP NBP2-45354H
Janelia Fluor 549 NBP2-45354JF549
Janelia Fluor 646 NBP2-45354JF646
PE NBP2-45354PE
PerCP NBP2-45354PCP

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Bioinformatics

Gene Symbol FOXA1
Uniprot