HepG2 Whole Cell Lysate

Images

 
Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].
SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, PAGE
Concentration
2.5 mg/ml

Order Details

HepG2 Whole Cell Lysate Summary

Specificity
HepG2

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-42569 in the following application:

Publications
Read Publication using NBP1-42569.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted. BME has not been added.
Concentration
2.5 mg/ml

Lysate Details for HepG2

Type
Cell
Tissue
Liver
Protein State
Native
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HepG2 Lysate (NBP1-42569)(1)

We have publications tested in 1 application: WB.


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WB
(1)
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Review for HepG2 Lysate (NBP1-42569) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42569:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 03/13/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman
SpeciesHuman

Blocking

Blocking Details5% non fat milk

Primary Anitbody

Dilution Ratio1 ug/ sample

Secondary Antibody

Secondary DescriptionHRP conjugated anti rabbit IgG
Secondary Concentration1:10000

Details

Detection NotesChemiluminescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HepG2 Lysate (NBP1-42569). (Showing 1 - 2 of 2 FAQ).

  1. How was this lysate prepared? Please advise as I am trying to use this material as a control lysate for a kit I purchased.
    • This lysate was prepared using standard RIPA buffer with 0.1% Triton X-100, and 1% SDS and sodium deoxycholate. We used mechanical separation to remove the cells from the plate and also use a cocktail of protease and phosphatase inhibitors in the lysate buffer.
  2. Good morning, we have to work with short-HepG2 Whole Cell Lysate 1.0mg to do adsorb on nanoparticles. We are chemical-physical. I would like to know if the sample handling under the hood (not flow alminare) will have a biohazard and what precautions should be taken.
    • NBP1-42569 is a whole cell lysate with the cells in a RIPA buffer. These cells are lysed and not longer viable. They are not considered a biohazard and no special precautions need to be taken.

Additional HepG2 Products

HepG2 NBP1-42569

Blogs on HepG2

There are no specific blogs for HepG2, but you can read our latest blog posts.

Contact Information

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Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

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Recent Reviews

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Anonymous
03/13/2014
Application: WB
Species: Human