HepG2 Whole Cell Lysate


Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].
SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IP, PAGE
2.5 mg/ml

Order Details

HepG2 Whole Cell Lysate Summary


Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 4X Laemmli Sample Buffer [62.5 mM Tris HCl, 1% LDS, 10% Glycerol, 0.005% bromophenol blue, pH 6.8] BME has not been added.
2.5 mg/ml


  • Western Blot
  • Immunoprecipitation
  • SDS-Page
Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Reviewed Applications
Read 1 Review rated 4
NBP1-42569 in the following applications:

Read Publication using NBP1-42569.

Lysate Details for HepG2

Protein State
Subcellular Fraction

Reactivity Notes



Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HepG2 Lysate (NBP1-42569)(1)

We have publications tested in 1 application: WB.

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Reviews for HepG2 Lysate (NBP1-42569) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-42569:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 03/13/2014


ApplicationWestern Blot
Sample TestedHuman


Blocking Details5% non fat milk

Primary Anitbody

Dilution Ratio1 ug/ sample

Secondary Antibody

Secondary DescriptionHRP conjugated anti rabbit IgG
Secondary Concentration1:10000


Detection NotesChemiluminescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HepG2 Lysate (NBP1-42569). (Showing 1 - 2 of 2 FAQ).

  1. How was this lysate prepared? Please advise as I am trying to use this material as a control lysate for a kit I purchased.
    • This lysate was prepared using standard RIPA buffer with 0.1% Triton X-100, and 1% SDS and sodium deoxycholate. We used mechanical separation to remove the cells from the plate and also use a cocktail of protease and phosphatase inhibitors in the lysate buffer.
  2. Good morning, we have to work with short-HepG2 Whole Cell Lysate 1.0mg to do adsorb on nanoparticles. We are chemical-physical. I would like to know if the sample handling under the hood (not flow alminare) will have a biohazard and what precautions should be taken.
    • NBP1-42569 is a whole cell lysate with the cells in a RIPA buffer. These cells are lysed and not longer viable. They are not considered a biohazard and no special precautions need to be taken.

Additional HepG2 Whole Cell Lysate Products

HepG2 NBP1-42569

Blogs on HepG2

There are no specific blogs for HepG2, but you can read our latest blog posts.

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