Leptin/OB Antibody

Images

 
Western Blot: Leptin/OB Antibody [NB300-611] - Analysis of leptin in mouse 3T3-L1.
Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611] - Staining of Leptin in sheep brain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, ShSpecies Glossary
Applications WB, IHC, IHC-Fr, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

Leptin/OB Antibody Summary

Immunogen
Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
Localization
Secreted
Specificity
Leptin
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LEP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 - 1:1000
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen 1:50 - 1:200
  • Immunocytochemistry 1:50 - 1:200
Application Notes
WB: Detects an approx. 16 kDa protein representing leptin from 3T3-L1 cell extract. IHC: Staining of leptin in sheep brain with NB300-611 results in staining of the ventromedial hypothalamus.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23270329). Human reactivity reported in scientific literature (PMID: 21983012). Porcine reactivity reported in scientific literature (PMID: 18413648). Avian reactivity reported in scientific literature (PMID: 16730725).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Leptin/OB Antibody

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LEP
Entrez
Uniprot