Mouse Laminin ELISA Kit (Colorimetric)

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ELISA: Laminin ELISA Kit [Biotin] [KA0380] - Standard curve

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Body Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
10 pg/mL

Order Details

Mouse Laminin ELISA Kit (Colorimetric) Summary

Description
Laminin (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse laminin.
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
10 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LAMA1

Applications/Dilutions

Application Notes
Useful for quantification.
Publications
Read Publications using KA0380.

Packaging, Storage & Formulations

Storage
Store at -20 °C.

Kit Components

Components
  1. Sample diluent buffer
  2. TMB color developing agent
  3. TMB stop solution
  4. Antibody diluent buffer
  5. ABC diluent buffer
  6. 96-well plate precoated with anti- mouse Laminin antibody
  7. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  8. Biotinylated anti-mouse Laminin antibody, dilution 1:100
  9. Lyophilized mouse Laminin standard

Notes

Quality control test: Standard curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse Laminin ELISA Kit (Colorimetric)

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kD. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kD) one B1 chain (215 kD) and one B2 chain (205 kD) all held together by disulfide bonds. The molecule has a cross-like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate-adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration. Quantitation of laminin is beneficial by allowing for a method of non-invasive testing. For example, in fibrosis, a serious and frequent complication of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver, the concentration of laminin in serum is positively related to the severity of the fibrotic liver disease being significantly higher in cirrhosis than in mere fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Laminin ELISA Kit (KA0380)(3)

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol LAMA1