Mouse Laminin ELISA Kit (Colorimetric)

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ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380] - Standard curve for Mouse Laminin ELISA Kit (Colorimetric) [KA0380].

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA, Quantification
Suitable Sample Type
Cell lysates, Cell Culture Supernatant, Plasma (EDTA, Heparin), Serum
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
10 pg/mL

Order Details

Mouse Laminin ELISA Kit (Colorimetric) Summary

Description
Laminin (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse laminin.
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
10 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
LAMA1

Applications/Dilutions

Application Notes
Useful for quantification.
Publications
Read Publications using KA0380.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. Sample diluent buffer
  2. TMB color developing agent
  3. TMB stop solution
  4. Biotinylated anti-mouse Laminin antibody, dilution 1:100
  5. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  6. Antibody diluent buffer
  7. ABC diluent buffer
  8. 96-well plate precoated with anti- mouse Laminin antibody
  9. Lyophilized mouse Laminin standard

Notes

Quality control test: Standard curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse Laminin ELISA Kit (Colorimetric)

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • PTBHS
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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KA0380
Species: Mu
Applications: ELISA, Quantification

Publications for Laminin ELISA Kit (KA0380)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Laminin ELISA Kit (KA0380). (Showing 1 - 4 of 4 FAQs).

  1. What research areas can this product be used in?
    • All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
  2. What is the theoretical molecular weight for your Laminin antibodies?
    • The theoretical molecular weight for Laminin antibodies is 337 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
    • The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol LAMA1