Laminin Antibody (LAM-89)


Immunohistochemistry-Paraffin: Laminin Antibody (LAM-89) [NB600-883] - Human placenta section.
Immunohistochemistry-Paraffin: Laminin Antibody (LAM-89) [NB600-883] - Human tongue section.

Product Details

Reactivity Hu, Po, Fe, Fi, Ca(-), Ch(-), Gp(-), Gt(-), Mu(-), Rb(-), Re(-), Rt(-), Sh(-)Species Glossary
Applications WB, ELISA, IHC, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

Laminin Antibody (LAM-89) Summary

Purified human laminin protein.
Extracellular. Found in the basement membranes.
Basement Membrane Marker
Specificity was determined by immunoperoxidase and immunofluorescent labeling of protease-digested, formalin-fixed, paraffin-embedded human or animal tissue sections, localizing specifically the basal membrane of blood vessels, epithelium, nerve, and muscle tissues. Localizes specifically in the basal membrane of blood vessels, epithelium, nerve and muscle fibers. Shows no cross-reaction with collagen type IV, fibronectin, vitronectin or chondroitin sulfates type A, B and C using a dot blot immunoassay. This antibody may be used for identification of laminin in various human tissues and bodily fluids.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Ascites fluid.
15mM Sodium Azide


  • Western Blot
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user. indirect ELISA: suitable
Read Publication using
NB600-883 in the following applications:

Reactivity Notes

Stains basal membranes of human, pig and cat. Fish reactivity reported in scientific literature (PMID: 26517388)

Alternate Names for Laminin Antibody (LAM-89)

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha


Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kD. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kD) one B1 chain (215 kD) and one B2 chain (205 kD) all held together by disulfide bonds. The molecule has a cross-like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate-adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration. Quantitation of laminin is beneficial by allowing for a method of non-invasive testing. For example, in fibrosis, a serious and frequent complication of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver, the concentration of laminin in serum is positively related to the severity of the fibrotic liver disease being significantly higher in cirrhosis than in mere fibrosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: WB
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC-P
Species: Hu, Po, Fe, Fi, Ca(-), Ch(-), Gp(-), Gt(-), Mu(-), Rb(-), Re(-), Rt(-), Sh(-)
Applications: WB, ELISA, IHC, IHC-P

Publications for Laminin Antibody (NB600-883)(1)

We have publications tested in 1 confirmed species: Fish.

We have publications tested in 1 application: IHC-P.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for Laminin Antibody (NB600-883) (0)

There are no reviews for Laminin Antibody (NB600-883). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Laminin Antibody (NB600-883) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Laminin Antibody (LAM-89) Products

Related Products by Gene

Bioinformatics Tool for Laminin Antibody (NB600-883)

Discover related pathways, diseases and genes to Laminin Antibody (NB600-883). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Laminin Antibody (NB600-883)

Discover more about diseases related to Laminin Antibody (NB600-883).

Pathways for Laminin Antibody (NB600-883)

View related products by pathway.

PTMs for Laminin Antibody (NB600-883)

Learn more about PTMs related to Laminin Antibody (NB600-883).

Research Areas for Laminin Antibody (NB600-883)

Find related products by research area.

Blogs on Laminin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol LAMA1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-883 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought