Laminin Antibody (LAM-89) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Extracellular. Found in the basement membranes.
Basement Membrane Marker
Laminin Antibody (LAM-89) specificity was determined by immunoperoxidase and immunofluorescent labeling of protease-digested, formalin-fixed, paraffin-embedded human or animal tissue sections, localizing specifically the basal membrane of blood vessels, epithelium, nerve, and muscle tissues. Localizes specifically in the basal membrane of blood vessels, epithelium, nerve and muscle fibers. Shows no cross-reaction with collagen type IV, fibronectin, vitronectin or chondroitin sulfates type A, B and C using a dot blot immunoassay. This antibody may be used for identification of laminin in various human tissues and bodily fluids.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in ICC/IF reported in scientific literature. Use in IHC-P reported in scientific literature (PMID: 26517388). Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user. indirect ELISA: suitable
337 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Should not react with carp, lizard, frog, snake. Stains basal membranes of human, pig and cat. Fish reactivity reported in scientific literature (PMID: 26517388). Human and mouse reactivity reported in scientific literature.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
15mM Sodium Azide
Alternate Names for Laminin Antibody (LAM-89)
Laminin A chain
laminin subunit alpha-1
laminin, alpha 1
Laminin-1 subunit alpha
Laminin-3 subunit alpha
S-laminin subunit alpha
Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).
1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214
2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567
3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Laminin Antibody (NB600-883). (Showing 1 - 4 of 4 FAQs).
What research areas can this product be used in?
All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
What is the theoretical molecular weight for your Laminin antibodies?
The theoretical molecular weight for Laminin antibodies is 337 kDa.
If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.