Laminin Antibody (LAM-89)

Images

 
Immunohistochemistry-Paraffin: Laminin Antibody (LAM-89) [NB600-883] - Analysis in human placenta section with Laminin Antibody (LAM-89) [NB600-883].
Immunohistochemistry-Paraffin: Laminin Antibody (LAM-89) [NB600-883] - Analysis in human tongue section with Laminin Antibody (LAM-89) [NB600-883].

Product Details

Summary
Reactivity Hu, Mu, Po, Fe, Fi, Ca(-), Ch(-), Gp(-), Gt(-), Rb(-), Re(-), Rt(-), Sh(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
LAM-89
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Laminin Antibody (LAM-89) Summary

Immunogen
Laminin Antibody (LAM-89) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Localization
Extracellular. Found in the basement membranes.
Marker
Basement Membrane Marker
Specificity
Laminin Antibody (LAM-89) specificity was determined by immunoperoxidase and immunofluorescent labeling of protease-digested, formalin-fixed, paraffin-embedded human or animal tissue sections, localizing specifically the basal membrane of blood vessels, epithelium, nerve, and muscle tissues. Localizes specifically in the basal membrane of blood vessels, epithelium, nerve and muscle fibers. Shows no cross-reaction with collagen type IV, fibronectin, vitronectin or chondroitin sulfates type A, B and C using a dot blot immunoassay. This antibody may be used for identification of laminin in various human tissues and bodily fluids.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAMA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
Use in ICC/IF reported in scientific literature. Use in IHC-P reported in scientific literature (PMID: 26517388). Can be used for protease-digested formalin-fixed, paraffin embedded human or animal tissue sections. Localizes the basal membrane of blood vessels, epithelium, nerve, and muscle fibers. IHC-P: This concentration was determined by immunoperoxidase staining of protease-digested human tissue. Antigen unmasking is critical in using this product. Optimal dilutions/concentrations should be determined by the end user. indirect ELISA: suitable
Theoretical MW
337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-883 in the following applications:

Reactivity Notes

Should not react with carp, lizard, frog, snake. Stains basal membranes of human, pig and cat. Fish reactivity reported in scientific literature (PMID: 26517388). Human and mouse reactivity reported in scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for Laminin Antibody (LAM-89)

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • PTBHS
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-883
Species: Hu, Mu, Po, Fe, Fi, Ca(-), Ch(-), Gp(-), Gt(-), Rb(-), Re(-), Rt(-), Sh(-)
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for Laminin Antibody (NB600-883)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Fish.

We have publications tested in 2 applications: ICC/IF, IHC-P.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Fish
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NB600-883 Applications Species
Gurcu B, Koca YB, Ozkut M, Tuglu MI. Matrix changes due to the toxic effects of metronidazole in intestinal tissue of fish (Onchorhynchus mykiss). Chemosphere. 2015 Oct 24 [PMID: 26517388] (IHC-P, Fish) IHC-P Fish
Khan M, Ashraf M Human iPS Cells Derived Skeletal Muscle Progenitor Cells Promote Myoangiogenesis and Restore Dystrophin in Duchenne Muscular Dystrophic Mice bioRxiv Jan 1 2020 (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Balan E, De Groote E, Bouillon M et al. No effect of the endurance training status on senescence despite reduced inflammation in skeletal muscle of older individuals Am. J. Physiol. Endocrinol. Metab. Jul 21 2020 [PMID: 32691630] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB600-883). (Showing 1 - 4 of 4 FAQs).

  1. What research areas can this product be used in?
    • All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
  2. What is the theoretical molecular weight for your Laminin antibodies?
    • The theoretical molecular weight for Laminin antibodies is 337 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
    • The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

Secondary Antibodies

 

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol LAMA1
Entrez