Lactate Dehydrogenase C Antibody

Images

 
Western Blot: Lactate Dehydrogenase C Antibody [NB100-68160] - (1ug/ml) staining of Mouse Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68160] - Staining of Mouse Testis. (1ug/ml). Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68160] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68160] - (5ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Lactate Dehydrogenase C Antibody Summary

Immunogen
Peptide with sequence C-DSDKEHWKNVHKQ corresponding to internal region according to NP_002292.1, NP_059144.1.
Epitope
DSDKEHWKNVHKQ
Specificity
Both variants represent identical product (NP_002292.1 and NP_059144.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LDHC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-6 ug/ml
  • Immunohistochemistry-Paraffin 5-6 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.3-1ug/ml
Application Notes
Western blot: Approx 35kDa band observed in Mouse Testis lysates (calculated MW of 35.9kDa according to Mouse NP_038608.1). Primary incubation was 1 hour.
Control
Lactate Dehydrogenase C Overexpression Lysate
Publications
Read Publication using NB100-68160.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase C Antibody

  • Cancer/testis antigen 32
  • CT32EC 1.1.1.27
  • EC 1.1.1
  • lactate dehydrogenase C
  • lactate dehydrogenase C4
  • LDH testis subunit
  • LDH3MGC111073
  • LDH-C
  • LDHX
  • LDH-X
  • L-lactate dehydrogenase C chain

Background

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase C Antibody (NB100-68160)(1)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LDHC
Entrez
Uniprot