Lactate Dehydrogenase C Antibody

Images

 
Western Blot: Lactate Dehydrogenase C Antibody [NB100-68160] - Staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.03ug/ml)
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NB100-68160] - Staining of Mouse Testis. (1ug/ml). Data kindly provided by Dr. Erwin Goldberg, Northwestern University, Evanston, IL USA.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Lactate Dehydrogenase C Antibody Summary

Immunogen
Peptide with sequence C-DSDKEHWKNVHKQ corresponding to internal region according to NP_002292.1, NP_059144.1.
Epitope
DSDKEHWKNVHKQ
Specificity
Both variants represent identical product (NP_002292.1 and NP_059144.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LDHC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 30-35 kDa band observed in mouse testis lysates (calculated MW of 36.6 kDa band according to human NP_002292.1 and 35.9 kDa band according to mouse NP_038608.1). IHC: Strong signal in seminiferous tubules of mouse testis
Positive Control
Lactate Dehydrogenase C Lysate (NBL1-12473)
Publications
Read Publication using NB100-68160.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase C Antibody

  • Cancer/testis antigen 32
  • CT32EC 1.1.1.27
  • EC 1.1.1
  • lactate dehydrogenase C
  • lactate dehydrogenase C4
  • LDH testis subunit
  • LDH3MGC111073
  • LDH-C
  • LDHX
  • LDH-X
  • L-lactate dehydrogenase C chain

Background

Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase C Antibody (NB100-68160)(1)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LDHC
Entrez
Uniprot