Lactate Dehydrogenase B Antibody

Images

 
Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP1-31945] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP1-31945] - Paraffin-embedded gastric carcinoma; antibody dilution 1:200.
Western Blot: Lactate Dehydrogenase B Antibody [NBP1-31945] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Mk, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

Lactate Dehydrogenase B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LDH-B. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (96%), Bovine (98%), Rabbit (100%), Monkey (100%), Primate (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%) and Chimpanzee (100%), Chicken (87%), Xenopus laevis (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.3 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase B Antibody

  • EC 1.1.1
  • EC 1.1.1.27
  • Epididymis Secretory Protein Li 281
  • HEL-S-281
  • Lactate Dehydrogenase B
  • LDH heart subunit
  • LDHB
  • LDH-B
  • LDHBD
  • LDH-H
  • L-lactate dehydrogenase B chain
  • Renal Carcinoma Antigen NY-REN-46
  • TRG-5

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LDHB
Entrez