Lactate Dehydrogenase Antibody - BSA Free

Immunoprecipitation:Lactate Dehydrogenase AntibodyNBP3-30578] -Detection of human Lactate Dehydrogenase B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lactate Dehydrogenase B antibody NBP3-30578 (lot NBP3-30578-1) used for IP at 6 µg per reaction. Lactate Dehydrogenase B was also immunoprecipitated by rabbit anti-Lactate Dehydrogenase B antibody BL18400. For blotting immunoprecipitated Lactate Dehydrogenase B, NBP3-30578 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lactate Dehydrogenase Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Lactate Dehydrogenase Antibody - BSA Free (NBP3-30578) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 284 and 334
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDHB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Lactate Dehydrogenase Antibody - BSA Free

• EC 1.1.1
• EC 1.1.1.27
• lactate dehydrogenase B
• LDH heart subunit
• LDH-B
• LDH-H
• L-lactate dehydrogenase B chain
• Renal carcinoma antigen NY-REN-46
• TRG-5

Background

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDHA, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) -in the heart, LDH-2 (3H1M) -in the reticuloendothelial system, LDH-3 (2H2M) -in the lungs, LDH-4 (1H3M) -in the kidneys and LDH-5 (4M) -in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LDHB