Lactate Dehydrogenase A/LDHA Antibody - Azide Free

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Human lung papillory adenocarcinoma, using LDHA antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LDHA protein stained by LDHA antibody (NBP2-19320) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.

LDHA antibody detects LDHA protein at cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LDHA protein stained by LDHA antibody (NBP2-19320) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.

LDHA antibody detects LDHA protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LDHA antibody detects LDHA protein by western blot analysis.A. 30 ug PC-12 whole cell lysate/extractB. 30 ug Rat2 whole cell lysate/extract10% SDS-PAGELDHA antibody (NBP2-19320) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LDHA antibody detects LDHA protein by western blot analysis.A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug Raw264.7 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract10% SDS-PAGELDHA antibody (NBP2-19320) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LDHA antibody (NBP2-19320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Rb, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Lactate Dehydrogenase A/LDHA Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Lactate Dehydrogenase A/LDHA Antibody - Azide Free (NBP2-19320) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Lactate Dehydrogenase A/LDHA Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase A/LDHA. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (90%), Chimpanzee (100%), Bovine (91%), Rabbit (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDHA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody - Azide Free

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP2-19320)(1)

Publication using NBP2-19320

• Silagi ES, Novais EJ, Bisetto S et al. Lactate Efflux from Intervertebral Disc Cells is Required for Maintenance of Spine Health J. Bone Miner. Res. 2019-11-06 [PMID: 31692093] (IF/IHC, IHC-P, Mouse)

Bioinformatics

• Gene Symbol: LDHA
• Uniprot:
  • P00338(Human)