Rabbit IgG Isotype Control [Alexa Fluor® 488]

Images

 
Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor 488] [NBP2-24982] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

Order Details

Rabbit IgG Isotype Control [Alexa Fluor® 488] Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHG1
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ul/1 million cells
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antiobdy. Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24982 in the following applications:

Publications
Read Publications using
NBP2-24982 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50 mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for Rabbit IgG Isotype Control [Alexa Fluor® 488]

  • IgG

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for IgG Isotype Control (NBP2-24982)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow-CS, ICC/IF.


Filter By Application
Flow-CS
(1)
ICC/IF
(2)
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP2-24982 Applications Species
Takanashi Naoya, Tomosada Yohsuke, Villena Julio et al. Advanced application of bovine intestinal epithelial cell line for evaluating regulatory effect of lactobacilli against heat-killed enterotoxigenic Escherichia coli-mediated inflammation. BMC Microbiol. 2013 [PMID: 23497067] (ICC/IF) ICC/IF
Turco F, Sarnelli G, Cirillo C et al. Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut. 2014 Jan 1 [PMID: 23292665] (ICC/IF, Human)

Details:
To test any non-specific antigen-binding sites, additional experiments were performed using specific isotype antibody controls at the same concentration as the primary antibodies.
ICC/IF Human
Shimazu T, Villena J, Tohno M et al. Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infect Immun. 2012 Jan [PMID: 22083706] (Flow-CS)

Details:
Cat no 20304E (normal rabbit IgG isotype control): Flow (cell surface), Fig 4C (porcine intestinal epitheliocyte cell line).
Flow-CS
Wu J, Meng Z, Jiang M et al. Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific. Immunology. 2010 Mar [PMID: 19922426]
Patole PS, Pawar RD, Lech M et al. Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice. Nephrol Dial Transplant. 2006 Nov [PMID: 16954173]

Details:
Antibodies cited: For IHC (frozen) on mouse kidney sections (Fig. 5): TLR3 (IMG-516), TLR7 (IMG-581A), TLR9 (IMG-431) and Rabbit IgG isotype control antibody (20304). For Flow (Intacellular) and Flow (Cell surface) on J774 mouse macrophage-like cell line

Review for IgG Isotype Control (NBP2-24982) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using NBP2-24982:
Filter by Applications
ICC/IF
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG NBP2-24982
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reviewed by:
Anonymous
ICC/IF 02/01/2018
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotB6112117AF488

Comments

CommentsThis antibody served as a negative control for ICC staining.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for IgG Isotype Control (NBP2-24982) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Alexa Fluor 488 NBP2-24982
Alexa Fluor 647 NBP2-24891AF647
Alexa Fluor 700 NBP2-24891AF700
Allophycocyanin/Cy7 NBP2-24891APCCY7
DyLight 488 NBP2-24891G
FITC NBP2-24892
PE NBP2-24983

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
02/01/2018
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol IGHG1