Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.

Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free [NBP2-71121] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, IHC, IP
• Clone: OTI2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LDHA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000
• Theoretical MW: 36.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) - Azide and BSA Free

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LDHA