Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

Images

 
Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; ...read more
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and ...read more
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal ...read more
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
OTI2D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) Summary

Immunogen
Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000
Theoretical MW
36.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-47822 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody (OTI2D11)

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-47822)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-47822) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71121AF350
Alexa Fluor 405 NBP2-71121AF405
Alexa Fluor 488 NBP2-71121AF488
Alexa Fluor 532 NBP2-71121AF532
Alexa Fluor 594 NBP2-71121AF594
Alexa Fluor 647 NBP2-71121AF647
Alexa Fluor 700 NBP2-71121AF700
Alexa Fluor 750 NBP2-71121AF750
Biotin NBP2-71121B
DyLight 350 NBP2-71121UV
DyLight 405 NBP2-71121V
DyLight 488 NBP2-71121G
DyLight 550 NBP2-71121R
DyLight 594 NBP2-71121DL594
DyLight 650 NBP2-71121C
DyLight 680 NBP2-71121FR
DyLight 755 NBP2-71121IR
FITC NBP2-71121F
HRP NBP2-71121H
Janelia Fluor 549 NBP2-71121JF549
Janelia Fluor 646 NBP2-71121JF646

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Bioinformatics

Gene Symbol LDHA
Entrez