JMJD2B Antibody

Images

 
Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody ...read more
Western Blot: JMJD2B Antibody [NB100-74605] - Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Dilution used for WB at 0.5 ug/ml.
Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74604.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

JMJD2B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1056 and 1096 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB100-74605 in the following applications:

Publications
Read Publications using
NB100-74605 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD2B Antibody

  • EC 1.14.11
  • FLJ44906
  • JHDM3B
  • JmjC domain-containing histone demethylation protein 3B
  • JMJD2BEC 1.14.11.-
  • jumonji domain containing 2B
  • Jumonji domain-containing protein 2B
  • KIAA0876lysine-specific demethylase 4B
  • lysine (K)-specific demethylase 4B

Background

JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JMJD2B Antibody (NB100-74605)(3)

Review for JMJD2B Antibody (NB100-74605) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-74605:
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WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for JMJD2B Antibody (NB100-74605) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for JMJD2B Antibody (NB100-74605)

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Recent Reviews

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Anonymous
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM4B
Entrez
Uniprot