G9a/EHMT2 Antibody (A8620A) [Unconjugated]

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ELISA
• Clone: A8620A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23005

G9a/EHMT2 Antibody (A8620A) [Unconjugated] Summary

• Immunogen: Recombinant mouse G9a/EHMT2
  aa 15-114
• Specificity: This antibody specifically recognizes mouse G9a and cross-reacts with human G9a. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EHMT2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Direct ELISA
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for G9a/EHMT2 Antibody (A8620A) [Unconjugated]

• ankyrin repeat-containing protein
• BAT8
• C6orf30
• chromosome 6 open reading frame 30
• DKFZp686H08213
• EC 2.1.1.-
• EC 2.1.1.43
• EHMT2
• euchromatic histone-lysine N-methyltransferase 2HLA-B-associated transcript 8
• FLJ35547
• G9A histone methyltransferase
• G9a
• G9AEm:AF134726.3
• H3-K9-HMTase 3
• Histone H3-K9 methyltransferase 3
• histone-lysine N-methyltransferase, H3 lysine-9 specific 3
• HLA-B associated transcript 8
• KMT1CNG36/G9a
• Lysine N-methyltransferase 1C
• NG36
• Protein G9a

Background

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for G9a/EHMT2 Antibody (PP-A8620A-00)(11)

Publications using PP-A8620A-00

• Able, AA;Richard, AJ;Stephens, JM; TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1) Biology 2023-04-30 [PMID: 37237488] (Western Blot, Mouse)
• LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122 Cancers, 2021-05-14;13(10):. 2021-05-14 [PMID: 34069116] (IHC, Human)
• T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis Cell Death & Disease, 2021-01-19;12(1):99. 2021-01-19 [PMID: 33468997] (IHC, Western Blot, Mouse)
• Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell Cyclin D1 integrates G9a-mediated histone methylation Oncogene, 2019-02-04;0(0):. 2019-02-04 [PMID: 30718920] (ChIP, ICC, Western Blot, Mouse)
• L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions Cell Death Dis, 2018-05-01;9(5):480. 2018-05-01 [PMID: 29691366] (Western Blot, Human)
• RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 2017-07-07;8(37):62081-62098. 2017-07-07 [PMID: 28977928] (IHC, Human)
• CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma Mol. Cancer Ther, 2017-03-06;0(0):. 2017-03-06 [PMID: 28265008] (IHC-P, Human)
• Li , Kai-Chun, Hua , Kuo-Tai, Lin , Yi-Shen, Su , Chia-Yi, Ko , Jenq-Yuh, Hsiao , Michael, Kuo , Min-Lian, Tan , Ching-Ti Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma. Mol Cancer, 2014-07-15;13(0):172. 2014-07-15 [PMID: 25027955] (IHC, Human)
• Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev, 2014-02-15;28(4):317-27. 2014-02-15 [PMID: 24532712] (Western Blot, Human)
• Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM. Cancer Res., 2010-10-12;70(20):7830-40. 2010-10-12 [PMID: 20940408] (IHC-P, Human)
• Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity. EMBO J, 2008-09-25;27(20):2691-701. 2008-09-25 [PMID: 18818693] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: EHMT2