G9a/EHMT2 Antibody (A8620A) [Unconjugated] Summary
Immunogen
Recombinant mouse G9a/EHMT2 aa 15-114
Specificity
This antibody specifically recognizes mouse G9a and cross-reacts with human G9a. Not yet tested in other species.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EHMT2
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Applications/Dilutions
Dilutions
Direct ELISA Immunohistochemistry Immunoprecipitation Western Blot
Publications
Read Publications using PP-A8620A-00 in the following applications:
Packaging, Storage & Formulations
Storage
This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70°C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer
A liquid formulation in physiologic saline with 0.1% NaN3 .
Preservative
0.1% Sodium Azide
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for G9a/EHMT2 Antibody (A8620A) [Unconjugated]
Background
Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for mouse embryogenesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Applications: WB
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Publications for G9a/EHMT2 Antibody (PP-A8620A-00)(18)
We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.
We have publications tested in 6 applications: ChIP, ICC, IHC, IHC-P, Immunohistochemistry, Western Blot.
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Showing Publications 1 -
10 of 18.
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Publications using PP-A8620A-00
Applications
Species
Huang, Z;Cui, W;Ratnayake, I;Tawil, R;Pfeifer, GP; SMCHD1 maintains heterochromatin and genome compartments in human myoblasts bioRxiv : the preprint server for biology 2024-07-10 [PMID: 39026812] (Western Blot, Human)
Western Blot
Human
Hsu, CM;Chang, KC;Chuang, TM;Chu, ML;Lin, PW;Liu, HS;Kao, SY;Liu, YC;Huang, CT;Wang, MH;Yeh, TJ;Gau, YC;Du, JS;Wang, HC;Cho, SF;Hsiao, CE;Tsai, Y;Hsiao, SY;Hung, LC;Yen, CH;Hsiao, HH; High G9a Expression in DLBCL and Its Inhibition by Niclosamide to Induce Autophagy as a Therapeutic Approach Cancers 2023-08-17 [PMID: 37627178] (IHC, Human)
IHC
Human
Able, AA;Richard, AJ;Stephens, JM; TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1) Biology 2023-04-30 [PMID: 37237488] (Western Blot, Mouse)
Western Blot
Mouse
LT Yuan, WJ Lee, YC Yang, BR Chen, CY Yang, MW Chen, JQ Chen, M Hsiao, MH Chien, KT Hua Histone Methyltransferase G9a-Promoted Progression of Hepatocellular Carcinoma Is Targeted by Liver-Specific Hsa-miR-122 Cancers, 2021-05-14;13(10):. 2021-05-14 [PMID: 34069116] (IHC, Human)
IHC
Human
T Nakatsuka, K Tateishi, H Kato, H Fujiwara, K Yamamoto, Y Kudo, H Nakagawa, Y Tanaka, H Ijichi, T Ikenoue, T Ishizawa, K Hasegawa, M Tachibana, Y Shinkai, K Koike Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis Cell Death & Disease, 2021-01-19;12(1):99. 2021-01-19 [PMID: 33468997] (IHC, Western Blot, Mouse)
IHC, Western Blot
Mouse
Tian H, Billings T, Walker M et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis Mol Biol Cell 2020-11-11 [PMID: 33175657]
Z Li, X Jiao, G Di Sante, A Ertel, MC Casimiro, M Wang, S Katiyar, X Ju, DV Klopfenste, A Tozeren, W Dampier, I Chepelev, A Jeltsch, RG Pestell Cyclin D1 integrates G9a-mediated histone methylation Oncogene, 2019-02-04;0(0):. 2019-02-04 [PMID: 30718920] (ChIP, ICC, Western Blot, Mouse)
ChIP, ICC, Western Blot
Mouse
L Li, W Liu, H Wang, Q Yang, L Zhang, F Jin, Y Jin Mutual inhibition between HDAC9 and miR-17 regulates osteogenesis of human periodontal ligament stem cells in inflammatory conditions Cell Death Dis, 2018-05-01;9(5):480. 2018-05-01 [PMID: 29691366] (Western Blot, Human)
Western Blot
Human
RJ Chen, CT Shun, ML Yen, CH Chou, MC Lin Methyltransferase G9a promotes cervical cancer angiogenesis and decreases patient survival Oncotarget, 2017-07-07;8(37):62081-62098. 2017-07-07 [PMID: 28977928] (IHC, Human)
IHC
Human
Ozge Gursoy-Yuzugullu, Chelsea Carman, Rodolfo Bortolozo Serafim, Marios Myronakis, Valeria Valente, Brendan D. Price Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity Oncotarget 2017-04-11 [PMID: 28445939]
CW Liu, KT Hua, KC Li, HF Kao, RL Hong, JY Ko, M Hsiao, ML Kuo, CT Tan Histone methyltransferase G9a drives chemotherapy resistance by regulating the glutamate-cysteine ligase catalytic subunit in head and neck squamous cell carcinoma Mol. Cancer Ther, 2017-03-06;0(0):. 2017-03-06 [PMID: 28265008] (IHC-P, Human)
IHC-P
Human
Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al. Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆ Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2016-07-01 [PMID: 26952936]
Ghislain Auclair, Julie Borgel, Lionel A. Sanz, Judith Vallet, Sylvain Guibert, Michael Dumas et al. EHMT2 directs DNA methylation for efficient gene silencing in mouse embryos Genome Research 2016-02-01 [PMID: 26576615]
Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee et al. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development eLife 2015-11-09 [PMID: 26551560] (Immunohistochemistry, Transgenic Mouse)
Immunohistochemistry
Transgenic Mouse
Li , Kai-Chun, Hua , Kuo-Tai, Lin , Yi-Shen, Su , Chia-Yi, Ko , Jenq-Yuh, Hsiao , Michael, Kuo , Min-Lian, Tan , Ching-Ti Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma. Mol Cancer, 2014-07-15;13(0):172. 2014-07-15 [PMID: 25027955] (IHC, Human)
IHC
Human
Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F The methyltransferase G9a regulates HoxA9-dependent transcription in AML. Genes Dev, 2014-02-15;28(4):317-27. 2014-02-15 [PMID: 24532712] (Western Blot, Human)
Western Blot
Human
Chen MW, Hua KT, Kao HJ, Chi CC, Wei LH, Johansson G, Shiah SG, Chen PS, Jeng YM, Cheng TY, Lai TC, Chang JS, Jan YH, Chien MH, Yang CJ, Huang MS, Hsiao M, Kuo ML H3K9 histone methyltransferase G9a promotes lung cancer invasion and metastasis by silencing the cell adhesion molecule Ep-CAM. Cancer Res., 2010-10-12;70(20):7830-40. 2010-10-12 [PMID: 20940408] (IHC-P, Human)
IHC-P
Human
Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity. EMBO J, 2008-09-25;27(20):2691-701. 2008-09-25 [PMID: 18818693] (Western Blot, Mouse)
Western Blot
Mouse
Show All 18 Publications.
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