Cyclin B1 Antibody [Unconjugated]


Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. more
Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) at 10 µg/mL overnight at 4 more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclin B1 at more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC

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Cyclin B1 Antibody [Unconjugated] Summary

E. coli-derived recombinant human Cyclin B1
Accession # P14635
Detects human and mouse Cyclin B1 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Read Publications using
AF6000 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyclin B1 Antibody [Unconjugated]

  • CCNB
  • CCNB1
  • Cyclin B1
  • G2/mitotic-specific cyclin B1
  • G2/mitotic-specific cyclin-B1


Cyclin B1 (also CCNB1 and G2/mitotic-specific cyclin-B1) is a member of the cyclin AB subfamily, cyclin family of proteins. Although its predicted MW is 50 kDa, it runs anomalously at 62 kDa in SDS-PAGE. Cyclin B1 associates with both CDK1 and 2 providing substrate specificity to a phosphorylating complex. A phosphor‑CDK1:Cyclin B1 complex is inactive and cytosolic during interphase. At the beginning of mitosis, CDK1 is dephosphorylated and activated, and the CDK1:Cyclin B1 complex initiates formation of the mitotic scaffold. Human Cyclin B1 is 433 amino acids (aa) in length. It contains two cyclin box folds (aa 201‑290 and 298‑383) and two substrate binding sites (aa 298‑342 and 343‑380). Phosphorylation occurs at Ser9, Ser35, Ser69, and Thr321. There is one potential alternative start site at Met252 and deletions of aa 363‑399 and 365‑433. Over aa 1‑91, human Cyclin B1 shares 63% aa identity with mouse Cyclin B1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: ELISA, IHC, IHC-P, IP, KD, S-ELISA, WB
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Species: Bv, Fe, Hu, Mu, Rt
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Species: Bv, Hu, Pm, Mu, Po, Rt
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Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
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Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
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Publications for Cyclin B1 Antibody (AF6000)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 6 of 6.
Publications using AF6000 Applications Species
N Krämer, M Schütz, UG Mato, L Herhaus, M Marschall, C Zimmermann Recombinant Human Cytomegalovirus Expressing an Analog-Sensitive Kinase pUL97 as Novel Tool for Functional Analyses Viruses, 2022;14(10):. 2022 [PMID: 36298840] (Bioassay, Human) Bioassay Human
M Schütz, R Müller, E Socher, C Wangen, F Full, E Wyler, D Wong, M Scherer, T Stamminger, S Chou, WD Rawlinson, ST Hamilton, H Sticht, M Marschall Highly Conserved Interaction Profiles between Clinically Relevant Mutants of the Cytomegalovirus CDK-like Kinase pUL97 and Human Cyclins: Functional Significance of Cyclin H International Journal of Molecular Sciences, 2022;23(19):. 2022 [PMID: 36233116] (Western Blot, Human) Western Blot Human
FA Moretti, G Giardino, TCH Attenborou, AS Gkazi, BK Margetts, G la Marca, L Fairbanks, T Crompton, HB Gaspar Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy Scientific Reports, 2021;11(1):23221. 2021 [PMID: 34853379] (Western Blot, Mouse) Western Blot Mouse
M Schütz, M Steingrube, E Socher, R Müller, S Wagner, M Kögel, H Sticht, M Marschall Functional Relevance of the Interaction between Human Cyclins and the Cytomegalovirus-Encoded CDK-Like Protein Kinase pUL97 Viruses, 2021;13(7):. 2021 [PMID: 34198986] (Immunoprecipitation, Human) Immunoprecipitation Human
T Uesato, T Ogihara, A Hara, H Iida, T Miyatsuka, Y Fujitani, S Takeda, H Watada Enhanced Expression of the Key Mitosis Regulator Cyclin B1 Is Mediated by PDZ-Binding Kinase in Islets of Pregnant Mice J Endocr Soc, 2018;2(3):207-219. 2018 [PMID: 29594255] (Western Blot, Mouse) Western Blot Mouse
Porrello , Emanuela, Rivellini , Cristina, Dina , Giorgia, Triolo , Daniela, Del Carro , Ubaldo, Ungaro , Daniela, Panattoni , Martina, Feltri , Maria La, Wrabetz , Lawrence, Pardi , Ruggero, Quattrini , Angelo, Previtali , Stefano Jab1 regulates Schwann cell proliferation and axonal sorting through p27. J Exp Med, 2014;211(1):29-43. 2014 [PMID: 24344238] (Western Blot, Mouse) Western Blot Mouse

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Gene Symbol CCNB1