Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

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Genetic Strategies: Knockout Validated: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Total cell extracts of wild-type (RPE1WT) and KDM3A gene-edited RPE1 mutants (KDM3ACr22, named ...read more
Independent Antibodies: Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Detection of Human JMJD1A/JHDM2A on HeLa whole cell lysate using NB100-77282. Lysine (K)-specific ...read more
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody [NB100-77282] - Human cancer cells stained with NB100-77282 and Alexa Fluor 488 conjugated goat-anti-rabbit secondary ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1271 and 1321 of human jumonji domain containing 1A (JmjC domain-containing histone demethylation protein 2A) using the numbering given in entry NP_060903.2 (GeneID 55818).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM3A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Chromatin Immunoprecipitation (ChIP)
  • Knockout Validated
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID: 19624751). Use in Immunohistochemistry reported in scientific literature (PMID: 30405805). This Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody is validated for ICC/IF from a verified customer review.
Control
Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-77282 in the following applications:

Publications
Read Publications using
NB100-77282 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24554764).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM2A
  • JHDM2AJMJD1AJHMD2A
  • JmjC domain-containing histone demethylation protein 2A
  • JMJD1
  • JMJD1A
  • jumonji C domain-containing histone demethylase 2A
  • jumonji domain containing 1
  • jumonji domain containing 1A
  • Jumonji domain-containing protein 1A
  • KDM3A
  • KIAA0742
  • KIAA0742DKFZp686A24246
  • Lysine (K)specific Demethylase 3A
  • Lysine (K)-specific Demethylase 3A
  • lysine-specific demethylase 3A
  • testis-specific protein A
  • TSGA
  • TSGADKFZp686P07111

Background

This gene encodes a zinc finger protein that contains a jumonji domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-77282
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IP, ChIP, KO

Publications for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ChIP, IHC, IP, WB.


Filter By Application
ChIP
(1)
IHC
(1)
IP
(2)
WB
(5)
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Human
(3)
Mouse
(3)
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Showing Publications 1 - 9 of 9.
Publications using NB100-77282 Applications Species
Kim J, Choi Sa, Kim J et Al. Lysine-specific demethylase 3A is important for autophagic occurrence Biochem. Biophys. Res. Commun. Mar 19 2020 [PMID: 32201075] (Human) Human
Li X, Oh S, Song H et al. A potential common role of the Jumonji C domain containing 1A histone demethylase and chromatin remodeler ATRX in promoting colon cancer. Oncol Lett Sep 24 2018 [PMID: 30405805] (WB, IHC, Human) WB, IHC Human
Yuan SGS Epigenetic Reprogramming in Tumor Plasticity Thesis Jan 1 2020 (IP) IP
Yeyati PL, Schiller R, Mali G et al. KDM3A coordinates actin dynamics with intraflagellar transport to regulate cilia stability. J. Cell Biol. 2017 Feb 28 [PMID: 28246120] (IP, WB, Human) IP, WB Human
Ueda J, Ho JC, Lee KL et al. The Hypoxia-Inducible Epigenetic Regulators Jmjd1a and G9a Provide a Mechanistic Link between Angiogenesis and Tumor Growth. Mol. Cell. Biol. 2014 Jul 28 [PMID: 25071150] (WB, Mouse)

Details:
JMJD1A antibody used in WB on wild-type (WT) and Jmjd1a knockout (KO1 and KO2) embryonic stem cells (Fig 2B), 1% hypoxia exposed WT ES cells (Fig 4A), WT or Jmjd1a KO ES allograft tumors (8D) and Jmjd1a KO ES cells stably transfected with G9a shRNA (Fig 10A).
WB Mouse
Kasioulis I, Syred HM, Tate P et al. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Mol. Biol. Cell 2014 Feb 20 [PMID: 24554764] (WB, Mouse) WB Mouse
Javierre B, Rodriguez-Ubreva J, Al-Shahrour F et al. Long-Range Epigenetic Silencing Associates with Deregulation of Ikaros Targets in Colorectal Cancer Cells. Mol Cancer Res. 2011 Jul 7. [PMID: 21737484]
Inagaki T, Tachibana M, Magoori K et al. Obesity and metabolic syndrome in histone demethylase JHDM2a-deficient mice. Genes Cells. 2009 Aug [PMID: 19624751] (ChIP, WB, Mouse) ChIP, WB Mouse
Cadot B, Brunetti M, Coppari S et al. Loss of Histone Deacetylase 4 Causes Segregation Defects during Mitosis of p53-Deficient Human Tumor Cells. Cancer Res;69(15):6074-6082. 2009 [PMID: 19622775]

Review for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-77282:
Filter by Applications
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A NB100-77282
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5
reviewed by:
Anonymous
Human 04/27/2021
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Summary

Sample TestedCancer Cells
SpeciesHuman
LotA1

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (NB100-77282). (Showing 1 - 1 of 1 FAQs).

  1. Would you please let me know if NB100-77282 is validated for ChIP?
    • NB100-77282 hasn't been tested for use in ChIP. Theoretically this antibody can work for ChIP if you get successful cross-linking of JMJD1A to K9 on H3. There should be enough JMJD1A present to be able to capture the quick de-methylation step and you'd only need a couple DNA strands to IP to be able to amplify by PCR. This antibody should work for ChIP but it will most likely IP a low amount of DNA and we can not guarantee it for use in ChIP.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-77282AF350
Alexa Fluor 405 NB100-77282AF405
Alexa Fluor 488 NB100-77282AF488
Alexa Fluor 532 NB100-77282AF532
Alexa Fluor 594 NB100-77282AF594
Alexa Fluor 647 NB100-77282AF647
Alexa Fluor 700 NB100-77282AF700
Alexa Fluor 750 NB100-77282AF750
Biotin NB100-77282B
DyLight 350 NB100-77282UV
DyLight 405 NB100-77282V
DyLight 488 NB100-77282G
DyLight 550 NB100-77282R
DyLight 594 NB100-77282DL594
DyLight 650 NB100-77282C
DyLight 680 NB100-77282FR
DyLight 755 NB100-77282IR
HRP NB100-77282H

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Recent Reviews

5
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1
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Anonymous
04/27/2021
Application:
Species: Human

Bioinformatics

Gene Symbol KDM3A
Entrez
Uniprot