JMJD2D Antibody

Images

 
Western Blot: JMJD2D Antibody [NBP1-03357] - Western Blot Image of anti-JMJD2D. Whole cell protein from PC3 (lane 1), SHSY5Y (lane 2), and HeLa (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ChIP, ICC/IF (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

JMJD2D Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human JMJD2D protein (within residues 460-520). [Swiss-Prot# Q6B0I6]
Localization
Nucleus
Predicted Species
Primate (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4D
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/ml
  • Chromatin Immunoprecipitation
Application Notes
This JMJD2D antibody is useful for Western blot, where a band is seen at ~55 kDa.Chromatin Immunoprecipitation was reported in scientific literature. This antibody is not useful for ICC/IF.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC9)
JMJD2D Lysate (NBL1-12105)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-03357 in the following applications:

Publications
Read Publication using
NBP1-03357 in the following applications:

Reactivity Notes

Human. Immunogen sequence has 92% homology to monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD2D Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • FLJ10251
  • JHDM3D
  • JmjC domain-containing histone demethylation protein 3D
  • JMJD2Dlysine-specific demethylase 4D
  • jumonji domain containing 2D
  • Jumonji domain-containing protein 2D
  • lysine (K)-specific demethylase 4D
  • MGC141909

Background

Jumonji lysine demethylases (JMJDs) are oxygenases catalyzing the demethylation of mono-, di-, or trimethylated lysines, and its members (JMJD2A, JMJD2B, JMJD2C, and JMJD2D/ KDM4D) are Fe(II)-/alpha-ketoglutarate-dependent enzymes harboring a JmjC catalytic domain. JMJD2 shows differential substrate specificities and unlike, JMJD2A, JMJD2B, and JMJD2C that efficiently demethylate H3K9me3 as well as H3K36me3, JMJD2D is specific for H3K9me3 site. These enzymes have been implicated in transcriptional regulation, cell-cycle progression, nuclear hormone signaling, embryonic stem cell self-renewal and development. Overexpression of several JMJD2 homologs has been linked to cancer e.g. JMJD2D, JMJD2A and JMJD2C are overexpressed in prostate cancer wherein they function as AR coactivators to upregulate AR target genes and JMJD2D has been shown to function as a pro-proliferative protein in colon cancer cells. JMJD2D has also been reported to interact with the ligand binding domain of AR and thereby activate AR gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JMJD2D Antibody (NBP1-03357)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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ChIP
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Human
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Review for JMJD2D Antibody (NBP1-03357) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-03357:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot JMJD2D NBP1-03357
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reviewed by:
Anonymous
WB Human 01/11/2012
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-435 cell lysates
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit-IgG, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL prime

Product General Protocols

View specific protocols for JMJD2D Antibody (NBP1-03357): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for JMJD2D Antibody (NBP1-03357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP1-03357V
FITC Labeled NBP1-03357F

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Bioinformatics Tool for JMJD2D Antibody (NBP1-03357)

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Recent Reviews

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2
0
1
0

Anonymous
01/11/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM4D
Entrez
Uniprot