| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61800-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22174-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38192-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56243-25ul | |||
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 uM concentrations for 24 h, and intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA and protein levels were examined by qPCR and western blotting, respectively. Data represent means +/- SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0009.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell and animal models. HepG2 cells were incubated with a mixture of palmitic acid (PA) and oleic acid (OA) (1:2 ratio) at 800 uM concentrations for 24 h, and intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA and protein levels were examined by qPCR and western blotting, respectively. Data represent means +/- SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: JMJD2B Antibody [NB100-74605] - Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Dilution used for WB at 0.5 ug/ml.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B on HeLa whole cell lysate using NB100-74605. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74604.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Detection of Human JMJD2B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-NB100-74605-img0006.jpg)
![Immunoprecipitation: JMJD2B Antibody [NB100-74605] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74605 (lot NB100-74605-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot NB100-74605-1) of this antibody For blotting immunoprecipitated JMJD2B, NB100-74605 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Immunoprecipitation-NB100-74605-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level and represses PPARgamma2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. The levels of JMJD2B, H3K9me, H3K9me2, and H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31953-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Knockdown-Validated-NB100-74605-img0008.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Gomisin N (GN) represses the expression of JMJD2B, PPAR gamma 2, & PPAR gamma 2 steatosis target genes in HepG2 cells. HepG2 cells were treated with GN (100 μM) for 12 h. (A) JMJD2B expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are given in Supplementary Figure S4. (B) PPAR gamma 2 expression was measured by qPCR. (C,D) The expression of PPAR gamma 2 steatosis target genes was assessed by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415395913.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415525238.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241612623.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Knockdown of JMJD2B reduces the intracellular TG level & represses PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. (B) Intracellular TG levels were measured by a TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. scramble RNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415525267.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - JMJD2B expression increases in hepatic steatotic cell & animal models. (A) HepG2 cells were incubated with a mixture of palmitic acid (PA) & oleic acid (OA) (1:2 ratio) at 800 μM concentrations for 24 h, & intracellular triglyceride (TG) levels were analyzed by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S1A. (B) HepG2 cells were treated with T0901317 (10 μM) for 24 h, & intracellular triglyceride (TG) levels were measured by a TG assay kit. JMJD2B mRNA & protein levels were examined by qPCR & western blotting, respectively. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. no treatment. The full-length western blots are presented in Supplementary Figure S1B. (C) Total RNAs were isolated from the livers of HFD-induced obese mice. The JMJD2B mRNA levels were assessed by qPCR. Data represent means ± SEM of 5 mice. *p < 0.05 vs. ND mice. ND: normal diet. HFD: high fat diet. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241612639.jpg)
![Western Blot: JMJD2B Antibody [NB100-74605] - Overexpression of JMJD2B increases intracellular TG levels & stimulates PPAR gamma 2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, & H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPAR gamma 2 expression was assessed by qPCR & western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPAR gamma 2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-310202415535110.jpg)
![Immunohistochemistry: JMJD2B Antibody [NB100-74605] - Adenovirus-mediated JMJD2B overexpression stimulated hepatic steatosis in vivo. C57BL/6 mice (8 weeks old) were injected with adenovirus Ad-GFP or Ad-JMJD2B. After injection, Ad-injected mice were fed a HFD for 2 weeks. (A) JMJD2B expression was determined by qPCR (B) Immunostaining of JMJD2B (scale bar = 50 μm). Representative photographs are shown. JMJD2B positive cells were counted in ten random areas at 400× magnification & analyzed by using image J. Quantification of immunostaining assay is represented as percentage of JMJD2B positive cells. (C) Hepatic triglyceride (TG) levels were measured by a TG assay kit. (D) Liver morphology, ORO & H&E staining (scale bar = 100 μm). Representative images are presented. (E) Serum total TG levels. (F) Serum total cholesterol levels. Data represent means ± SEM from 5 mice. *p < 0.05 vs. Ad-GFP-infected mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30214048), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74605_rabbit-polyclonal-jmjd2b-antibody-31020241555239.jpg)
![Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Western-Blot-NBP3-16639-img0003.jpg "Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunocytochemistry-Immunofluorescence-NBP3-16639-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunocytochemistry-Immunofluorescence-NBP3-16639-img0001.jpg)
![Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunoprecipitation analysis of 300ug extracts of HCT116 cells using 3ug JMJD2B antibody (NBP3-16639). Western blot was performed from the immunoprecipitate using JMJD2B antibody (NBP3-16639) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-8I0I1-Immunoprecipitation-NBP3-16639-img0004.jpg)
![Chromatin Immunoprecipitation- JMJD2B Antibody (8I0I1) [NBP3-16639] - Analysis of extracts of 5637; cells, using KDM4B/JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-chromatin-immunoprecipitation-276202384446..jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202511411487.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-5220251136516.jpg)
![Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [NBP3-16639] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202511374118.jpg)
![Chromatin Immunoprecipitation: JMJD2B Antibody (8I0I1) [JMJD2B] - Chromatin immunoprecipitation analysis of extracts of 5637; cells, using JMJD2B antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202517311039.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [JMJD2B] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16639_rabbit-jmjd2b-mab-8i0i1-52202517333899.jpg)
![Western Blot: JMJD2B Antibody (JE36-58) [NBP3-32494] - Western blot analysis of JMJD2B on different lysates with Rabbit anti-JMJD2B antibody (NBP3-32494) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT116 cell lysate (20 ug/Lane) Lane 3: SW480 cell lysate (20 ug/Lane) Lane 4: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32494) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32494_rabbit-jmjd2b-mab-je36-58-27820241631681.jpg "Western Blot: JMJD2B Antibody (JE36-58) [NBP3-32494] - Western blot analysis of JMJD2B on different lysates with Rabbit anti-JMJD2B antibody (NBP3-32494) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HCT116 cell lysate (20 ug/Lane) Lane 3: SW480 cell lysate (20 ug/Lane) Lane 4: Mouse testis tissue lysate (40 ug/Lane) Predicted band size: 121 kDa Observed band size: 150 kDa Exposure time: 42 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32494) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JMJD2B Antibody (JE36-58) [NBP3-32494] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-JMJD2B antibody (NBP3-32494) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32494) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32494_rabbit-jmjd2b-mab-je36-58-298202414115414.jpg)
![Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (JE36-58) [NBP3-32494] - Immunocytochemistry analysis of HeLa cells labeling JMJD2B with Rabbit anti-JMJD2B antibody (NBP3-32494) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-JMJD2B antibody (NBP3-32494) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32494_rabbit-jmjd2b-mab-je36-58-29202410484824.jpg)
![Western Blot: JMJD2B Antibody (3A6H6) [NBP2-61800] - Analysis using KDM4B mAb against human KDM4B (AA: 960-1096) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/JMJD2B-Antibody-3A6H6-Western-Blot-NBP2-61800-img0003.jpg "Western Blot: JMJD2B Antibody (3A6H6) [NBP2-61800] - Analysis using KDM4B mAb against human KDM4B (AA: 960-1096) recombinant protein. (Expected MW is 41 kDa)")
![Western Blot: JMJD2B Antibody (3A6H6) [NBP2-61800] - Analysis using KDM4B mAb against HEK293 (1) and KDM4B (AA: 960-1096)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-3A6H6-Western-Blot-NBP2-61800-img0004.jpg)
![ELISA: JMJD2B Antibody (3A6H6) [NBP2-61800] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/JMJD2B-Antibody-3A6H6-ELISA-NBP2-61800-img0001.jpg)
![ELISA: JMJD2B Antibody (3A6H6) [NBP2-61800] - Analysis of NIH/3T3 cells using KDM4B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/JMJD2B-Antibody-3A6H6-ELISA-NBP2-61800-img0002.jpg)
![Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 ug per reaction. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605. For blotting immunoprecipitated JMJD2B, NB100-74604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-NB100-74604-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 ug per reaction. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605. For blotting immunoprecipitated JMJD2B, NB100-74604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: JMJD2B Antibody (SR1194) [NBP3-22174] - Western blot analysis using NBP3-22174 in (1) SW480 cell lysate; (2) Mouse testis cell lysate.](http://images.novusbio.com/fullsize/nbp3-22174_rabbit-jmjd2b-mab-sr1194-6720239533716.jpg "Western Blot: JMJD2B Antibody (SR1194) [NBP3-22174] - Western blot analysis using NBP3-22174 in (1) SW480 cell lysate; (2) Mouse testis cell lysate.")
![Western Blot: JMJD2B Antibody [NBP3-38192] - Western blot analysis of lysates from Mouse brain, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38192_rabbit-jmjd2b-pab-10122024140433.jpg "Western Blot: JMJD2B Antibody [NBP3-38192] - Western blot analysis of lysates from Mouse brain, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: JMJD2B Antibody [NBP2-56243] - Staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56243-img0001.jpg "Immunocytochemistry/Immunofluorescence: JMJD2B Antibody [NBP2-56243] - Staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.")
![Western Blot: JMJD2B Overexpression Lysate (Adult Normal) [NBP2-10300] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JMJD2B.](http://images.novusbio.com/fullsize/JMJD2B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10300-img0001.jpg "Western Blot: JMJD2B Overexpression Lysate (Adult Normal) [NBP2-10300] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JMJD2B.")
![Western Blot: JMJD2B Antibody [H00023030-B01P] - Analysis of KDM4B expression in transfected 293T cell line by KDM4B polyclonal antibody. Lane 1: KDM4B transfected lysate(120.56 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/JMJD2B-Antibody-Western-Blot-H00023030-B01P-img0002.jpg "Western Blot: JMJD2B Antibody [H00023030-B01P] - Analysis of KDM4B expression in transfected 293T cell line by KDM4B polyclonal antibody. Lane 1: KDM4B transfected lysate(120.56 KDa). Lane 2: Non-transfected lysate.")