ITK Antibody

Immunocytochemistry/Immunofluorescence: ITK Antibody [NBP1-31732] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ITK stained by ITK antibody [N2C1], Internal diluted at 1:500.Blue: Fluoroshield with DAPI.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (NBP1-31732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (NBP1-31732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ITK Antibody Summary

• Description: Novus Biologicals Rabbit ITK Antibody (NBP1-31732) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Mouse (90%), Rat (91%), Rhesus Macaque (98%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ITK Antibody

• EC 2.7.10
• EC 2.7.10.2
• EMT
• EMTMGC126257
• homolog of mouse T-cell itk/tsk
• IL2-inducible T-cell kinase
• ITK
• Kinase EMT
• LYK
• LYKMGC126258
• PSCTK2
• T-cell-specific kinase
• Tcsk
• tyrosine-protein kinase ITK/TSK
• Tyrosine-protein kinase Lyk

Background

IL2-inducible T-cell kinase. This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ITK