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BTK [p Tyr223] Antibody - BSA Free

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Western Blot: BTK [p Tyr223] Antibody [NBP1-78295] - WB analysis of BTKpY223 on Ramos whole cell lysate with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess ...read more
Immunohistochemistry: BTK [p Tyr223] Antibody [NBP1-78295] - IHC analysis of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.09 mg/ml

Order Details

BTK [p Tyr223] Antibody - BSA Free Summary

Immunogen
A synthetic phosphorylated peptide made to a peptide surrounding amino acid 223 of the human BTK protein [UniProt Q06187]
Modification
p Tyr223
Localization
Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BTK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 25724205)
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 ug/ml
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control Peptide
BTK Peptide (NBP1-78295PEP)
Publications
Read Publications using
NBP1-78295 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BTK [p Tyr223] Antibody - BSA Free

  • Agammaglobulinaemia tyrosine kinase
  • AGMX1
  • AGMX1MGC126262
  • AT
  • ATKIMD1
  • B-cell progenitor kinase
  • BPK
  • Bruton agammaglobulinemia tyrosine kinase
  • Bruton tyrosine kinase
  • BTK
  • dominant-negative kinase-deficient Brutons tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • IMD1
  • PSCTK1
  • tyrosine-protein kinase BTK
  • XLA
  • XLAMGC126261

Background

Bruton's tyrosine kinase (BTK) takes part in B-cell ontogeny, differentiation, and signaling. A kinase member of the Tec family, it's signaling leads to cytoskeletal changes, as well as calcium, NF kappa B, and NFAT signaling. Defects in the gene cause X-linked agammaglobulinemia (XLA). Autophosphorylation can occur on Tyr-223 and Tyr-551. pY223 activates the protein that is inhibited by IBTK, and creates a docking site for SH2 domain proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-78295
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Publications for BTK Antibody (NBP1-78295)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, ICC/IF, IF/IHC, PLA, WB.


Filter By Application
FLOW
(1)
ICC/IF
(1)
IF/IHC
(1)
PLA
(1)
WB
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP1-78295 Applications Species
Rehman R, Miller M, Krishnamurthy SS et al. Met/HGFR triggers detrimental reactive microglia in TBI Cell reports 2022-12-27 [PMID: 36577378] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse
Zhang J FLT3/FLT3L signaling modulates splenic dendritic cells maturation upon traumatic brain injury and is enhanced by ethanol intoxication Thesis 2023-01-01 (ICC/IF, Mouse)

Details:
1:200 ICC/IF diltuion
ICC/IF Mouse
FlUmann R, RehkAmper T, Nieper P Et al. An Autochthonous Mouse Model of Myd88- and BCL2-Driven Diffuse Large B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities Blood Cancer Discov 2021-01-15 [PMID: 33447829] (PLA, Mouse)

Details:
Citation using the HRP version of this antibody.
PLA Mouse
Wang SP, Iwata S, Nakayamada S et al. Amplification of IL-21 signalling pathway through Bruton's tyrosine kinase in human B cell activation Rheumatology (Oxford) 2015-02-26 [PMID: 25724205] (FLOW, Human)

Details:
BTK [p Tyr223] antibody used for FLOW on human CD19+ B cells in PBMCs alongwith rabbit IgG isotype control (see full text for detailed protocol).
FLOW Human
Eda H, Santo L, Cirstea DD et al. A novel Bruton's tyrosine kinase inhibitor CC-292 in combination with the proteasome inhibitor carfilzomib impacts the bone microenvironment in a multiple myeloma model with resultant antimyeloma activity. Leukemia 2014-02-12 [PMID: 24518207] (WB, Human) WB Human
Gleixner KV, Peter B, Blatt K, Suppan V et al. Synergistic growth-inhibitory effects of ponatinib and midostaurin (PKC412) on neoplastic mast cells carrying KIT D816V Haematologica 2013-03-28 [PMID: 23539538] (WB, Human) WB Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BTK
Entrez
Uniprot