BTK [p Tyr223] Antibody

Images

 
Western Blot: BTK [p Tyr223] Antibody [NBP1-78295] - WB analysis of BTKpY223 on Ramos whole cell lysate with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess ...read more
Immunohistochemistry: BTK [p Tyr223] Antibody [NBP1-78295] - IHC analysis of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.09 mg/ml

BTK [p Tyr223] Antibody Summary

Immunogen
A synthetic phosphorylated peptide made to a peptide surrounding amino acid 223 of the human BTK protein [UniProt Q06187]
Modification
p Tyr223
Localization
Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BTK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This BTK [pY223] antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in FLOW cytometry reported in scientific literature ( PMID 25724205)
Control Peptide
Publications
Read Publications using
NBP1-78295 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.09 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BTK [p Tyr223] Antibody

  • Agammaglobulinaemia tyrosine kinase
  • AGMX1
  • AGMX1MGC126262
  • AT
  • ATKIMD1
  • B-cell progenitor kinase
  • BPK
  • Bruton agammaglobulinemia tyrosine kinase
  • Bruton tyrosine kinase
  • BTK
  • dominant-negative kinase-deficient Brutons tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • IMD1
  • PSCTK1
  • tyrosine-protein kinase BTK
  • XLA
  • XLAMGC126261

Background

Bruton's tyrosine kinase (BTK) takes part in B-cell ontogeny, differentiation, and signaling. A kinase member of the Tec family, it's signaling leads to cytoskeletal changes, as well as calcium, NF kappa B, and NFAT signaling. Defects in the gene cause X-linked agammaglobulinemia (XLA). Autophosphorylation can occur on Tyr-223 and Tyr-551. pY223 activates the protein that is inhibited by IBTK, and creates a docking site for SH2 domain proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5129
Species: Hu
Applications: WB
NBP1-32945
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37585
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NB100-1343
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
AF3206
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
NBP2-37578
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP2-38535
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46411
Species: Hu
Applications: WB, IHC
7270-IL/CF
Species: Hu
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-55408
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF

Publications for BTK Antibody (NBP1-78295)(3)

Reviews for BTK Antibody (NBP1-78295) (0)

There are no reviews for BTK Antibody (NBP1-78295). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BTK Antibody (NBP1-78295) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-78295AF350
Alexa Fluor 405 NBP1-78295AF405
Alexa Fluor 488 NBP1-78295AF488
Alexa Fluor 532 NBP1-78295AF532
Alexa Fluor 594 NBP1-78295AF594
Alexa Fluor 647 NBP1-78295AF647
Alexa Fluor 700 NBP1-78295AF700
Alexa Fluor 750 NBP1-78295AF750
Biotin NBP1-78295B
DyLight 350 NBP1-78295UV
DyLight 405 NBP1-78295V
DyLight 488 NBP1-78295G
DyLight 550 NBP1-78295R
DyLight 594 NBP1-78295DL594
DyLight 650 NBP1-78295C
DyLight 680 NBP1-78295FR
DyLight 755 NBP1-78295IR
FITC NBP1-78295F
HRP NBP1-78295H
Janelia Fluor 549 NBP1-78295JF549
Janelia Fluor 646 NBP1-78295JF646

Additional BTK Products

Bioinformatics Tool for BTK Antibody (NBP1-78295)

Discover related pathways, diseases and genes to BTK Antibody (NBP1-78295). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BTK Antibody (NBP1-78295)

Discover more about diseases related to BTK Antibody (NBP1-78295).
 

Pathways for BTK Antibody (NBP1-78295)

View related products by pathway.

PTMs for BTK Antibody (NBP1-78295)

Learn more about PTMs related to BTK Antibody (NBP1-78295).
 

Research Areas for BTK Antibody (NBP1-78295)

Find related products by research area.

Blogs on BTK.

Repurposing FDA-approved drugs to combat the rise of antibiotic resistance
By Beth Melson, MSAntibiotic resistance is a global threat to public health. Widespread, inappropriate use of antibiotics, such as to treat viral infections or promote growth in livestock, has led to increased incid...  Read full blog post.

CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BTK [p Tyr223] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol BTK
Entrez
Uniprot