BTK [p Tyr223] Antibody - BSA Free

Western Blot: BTK [p Tyr223] Antibody [NBP1-78295] - WB analysis of BTKpY223 on Ramos whole cell lysate with peptide competition: 1. ab only, 2. 200 molar excess unmodified immunizing peptide and 3. 200 molar excess modified immunizing peptide.

Immunohistochemistry: BTK [p Tyr223] Antibody [NBP1-78295] - IHC analysis of BTK in kidney cancer xenograft using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.09 mg/ml

BTK [p Tyr223] Antibody - BSA Free Summary

• Immunogen: A synthetic phosphorylated peptide made to a peptide surrounding amino acid 223 of the human BTK protein [UniProt Q06187]
• Modification: p Tyr223
• Localization: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 ug/ml
• Application Notes: This BTK [pY223] antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in FLOW cytometry reported in scientific literature ( PMID 25724205)
• Control Peptide:

  BTK Peptide (NBP1-78295PEP)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.09 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BTK [p Tyr223] Antibody - BSA Free

• Agammaglobulinaemia tyrosine kinase
• AGMX1
• AGMX1MGC126262
• AT
• ATKIMD1
• B-cell progenitor kinase
• BPK
• Bruton agammaglobulinemia tyrosine kinase
• Bruton tyrosine kinase
• BTK
• dominant-negative kinase-deficient Brutons tyrosine kinase
• EC 2.7.10
• EC 2.7.10.2
• IMD1
• PSCTK1
• tyrosine-protein kinase BTK
• XLA
• XLAMGC126261

Background

Bruton's tyrosine kinase (BTK) takes part in B-cell ontogeny, differentiation, and signaling. A kinase member of the Tec family, it's signaling leads to cytoskeletal changes, as well as calcium, NF kappa B, and NFAT signaling. Defects in the gene cause X-linked agammaglobulinemia (XLA). Autophosphorylation can occur on Tyr-223 and Tyr-551. pY223 activates the protein that is inhibited by IBTK, and creates a docking site for SH2 domain proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTK Antibody (NBP1-78295)(4)

Publications using NBP1-78295

• FlUmann R, RehkAmper T, Nieper P Et al. An Autochthonous Mouse Model of Myd88- and BCL2-Driven Diffuse Large B-cell Lymphoma Reveals Actionable Molecular Vulnerabilities Blood Cancer Discov 2021-01-15 [PMID: 33447829] (PLA, Mouse)
  
  Details:
  Citation using the HRP version of this antibody.
• Wang SP, Iwata S, Nakayamada S et al. Amplification of IL-21 signalling pathway through Bruton's tyrosine kinase in human B cell activation Rheumatology (Oxford) 2015-02-26 [PMID: 25724205] (FLOW, Human)
  
  Details:
  BTK [p Tyr223] antibody used for FLOW on human CD19+ B cells in PBMCs alongwith rabbit IgG isotype control (see full text for detailed protocol).
• Eda H, Santo L, Cirstea DD et al. A novel Bruton's tyrosine kinase inhibitor CC-292 in combination with the proteasome inhibitor carfilzomib impacts the bone microenvironment in a multiple myeloma model with resultant antimyeloma activity. Leukemia 2014-02-12 [PMID: 24518207] (WB, Human)
• Gleixner KV, Peter B, Blatt K, Suppan V et al. Synergistic growth-inhibitory effects of ponatinib and midostaurin (PKC412) on neoplastic mast cells carrying KIT D816V Haematologica 2013-03-28 [PMID: 23539538] (WB, Human)

Bioinformatics

• Gene Symbol: BTK
• Entrez:
  • 695(Human)
• Uniprot:
  • Q06187()