IRAK4 Antibody


Western Blot: IRAK4 Antibody [NBP1-77231] - WB detection of IRAK4 protein in HeLa whole cell lysate using IRAK4 antibody using 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions respectively.
Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody [NBP1-77231] - ICC-IF staining of IRAK4 protein on K562 cells using IRAK4 antibody at 10 ug/mL concentration.
Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody [NBP1-77231] - Immunocytochemical staining of IRAK-4 protein in K562 cells using IRAK4 antibody at 10 ug/ml concentration and HRP conjugated secondary - DAB more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC, IF
1 mg/ml

Order Details

IRAK4 Antibody Summary

Antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


Application Notes
IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 2 mg/ml. A 50 kDa band can be detected. Anti-IRAK-4 has no cross response to other IRAKs.
Control Peptide
IRAK4 Peptide (NBP1-77231PEP)

Reactivity Notes


Alternate Names for IRAK4 Antibody

  • EC 2.7.11
  • EC
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64


Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Blogs on IRAK4.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol IRAK4

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