IRAK4 Antibody

Images

 
Western Blot: IRAK4 Antibody [NBP1-77231] - WB detection of IRAK4 protein in HeLa whole cell lysate using IRAK4 antibody using 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions respectively.
Immunocytochemistry: IRAK4 Antibody [NBP1-77231] - Immunocytochemical staining of IRAK-4 protein in K562 cells using IRAK4 antibody at 10 ug/ml concentration and HRP conjugated secondary - DAB chromogen detection method.
Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody [NBP1-77231] - ICC-IF staining of IRAK4 protein on K562 cells using IRAK4 antibody at 10 ug/mL concentration.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IRAK4 Antibody Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4. The immunogen is located within the last 50 amino acids of IRAK-4.
Specificity
IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRAK4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunocytochemistry
  • Immunofluorescence
Application Notes
IRAK-4 antibody can be used for the detection of IRAK-4 by Western blot at 1 to 2 mg/ml. A 50 kDa band can be detected. Anti-IRAK-4 has no cross response to other IRAKs.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IRAK4 Peptide (NBP1-77231PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRAK4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK4
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64

Background

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on IRAK4.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

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Bioinformatics

Gene Symbol IRAK4
Entrez
Uniprot