IRAK4 Antibody - BSA Free

Western Blot: IRAK4 Antibody [NBP1-77231] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IRAK4-NBP1-77231 (1 ug/mL), IRAK4 (1 ug/mL), beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: IRAK4 Antibody [NBP1-77231] - Immunocytochemical staining of IRAK-4 protein in K562 cells using IRAK4 antibody at 10 ug/ml concentration and HRP conjugated secondary - DAB chromogen detection method.

Western Blot: IRAK4 Antibody [NBP1-77231] - WB detection of IRAK4 protein in HeLa whole cell lysate using IRAK4 antibody using 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions respectively.

Western Blot: IRAK4 Antibody [NBP1-77231] - Western Blot Validation in Human Cell Lines. Loading: 15 ug/ of lysates per lane. Antibodies: IRAK4 NBP1-77231 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231] - Validation of IRAK4 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRAK4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IRAK4 Antibody - BSA Free (NBP1-77231) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK-4. The immunogen is located within the last 50 amino acids of IRAK-4. Amino Acid Squence: KKVQQLLQEMTAS
• Specificity: IRAK-4 antibody is predicted to not cross-react with other members of the IRAK protein family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRAK4 Peptide (NBP1-77231PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (85%), Mouse: (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRAK4 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• interleukin-1 receptor-associated kinase 4
• IPD1
• IRAK4
• IRAK-4
• NY-REN-64
• REN64
• Renal carcinoma antigen NY-REN-64

Background

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kappaB and MAP kinase (MAPK) by IL-1R/TLR. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4. Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK4
• Uniprot:
  • AAM15772(Human)