p38 alpha Antibody [Unconjugated]

Images

 
Western blot shows recombinant human p38 beta, p38 gamma, p38δ and Recombinant Human Active p38 alpha (Catalog # 5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at ...read more
Orexin B, p38 alpha, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Rabbit Anti-Human/Mouse/Rat p38 alpha ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38 alpha knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

View Available Formulations
Catalog# & Formulation Size Price

p38 alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p38 alpha
Met1-Ser360
Accession # Q16539
Specificity
Detects human, mouse and rat p38 alpha. Does not detect recombinant human p38 beta, p38 gamma or p38δ.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
AF8691 in the following applications:

Publications
Read Publications using
AF8691 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 alpha Antibody [Unconjugated]

  • MAPK14
  • p38 alpha
  • SAPK2a

Background

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC, IHC-P, KO
7270-IL/CF
Species: Hu
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00005706-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, RNAi
NBP1-47839
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB1846
Species: Hu, Mu, Rt
Applications: WB, ICC, KO
NBP1-47833
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
NB100-56530
Species: Hu
Applications: WB
NBP2-67861
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF8691
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO

Publications for p38 alpha Antibody (AF8691)(3)

Review for p38 alpha Antibody (AF8691) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF8691:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot p38 alpha AF8691
Enlarge
reviewed by:
Anonymous
WB Human 10/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cells
SpeciesHuman
LotGIG0915111

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for p38 alpha Antibody (AF8691) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional p38 alpha Products

Bioinformatics Tool for p38 alpha Antibody (AF8691)

Discover related pathways, diseases and genes to p38 alpha Antibody (AF8691). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for p38 alpha Antibody (AF8691)

Discover more about diseases related to p38 alpha Antibody (AF8691).
 

Pathways for p38 alpha Antibody (AF8691)

View related products by pathway.

PTMs for p38 alpha Antibody (AF8691)

Learn more about PTMs related to p38 alpha Antibody (AF8691).

Blogs on p38 alpha

There are no specific blogs for p38 alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/24/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPK14
Uniprot