p38 alpha Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 alpha was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows recombinant human p38 beta , p38 gamma , p38δ and Recombinant Human Active p38 alpha (Catalog # 5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Orexin B, p38 alpha , and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38 alpha knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p38 alpha at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

p38 alpha Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human p38 alpha
  Met1-Ser360
  Accession # Q16539
• Specificity: Detects human, mouse and rat p38 alpha . Does not detect recombinant human p38 beta , p38 gamma or p38δ.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK14
• Purity Statement: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 alpha Antibody [Unconjugated]

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Background

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p38 alpha Antibody (AF8691)(6)

Publications using AF8691

• M ?witalska, B Filip-Psur, M Milczarek, M Psurski, A Moszy?ska, AM D?browska, M Gawro?ska, K Krzymi?ski, M Bagi?ski, R Bartoszews, J Wietrzyk Combined anticancer therapy with imidazoacridinone C-1305 and paclitaxel in human lung and colon cancer xenografts-Modulation of tumour angiogenesis Oncogene, 2022-06-14;0(0):. 2022-06-14 [PMID: 35701366] (Capillary Immunoblot, Xenograft)
• SA Harper, JR Bassler, S Peramsetty, Y Yang, LM Roberts, D Drummer, RT Mankowski, C Leeuwenbur, K Ricart, RP Patel, MM Bamman, SD Anton, BC Jaeger, TW Buford Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial Exp Gerontol, 2020-10-15;0(0):111111. 2020-10-15 [PMID: 33068691] (Flow Cytometry, Human)
• M Iba, C Kim, J Florio, M Mante, A Adame, E Rockenstei, S Kwon, R Rissman, E Masliah Role of Alterations in Protein Kinase p38&gamma in the Pathogenesis of the Synaptic Pathology in Dementia With Lewy Bodies and &alpha-Synuclein Transgenic Models Front Neurosci, 2020-03-31;14(0):286. 2020-03-31 [PMID: 32296304] (IHC, Human, Mouse)
• Michael Torzewski Selective p38? MAP kinase/MAPK14 inhibition in enzymatically-modified LDL-stimulated human monocytes: implications for atherosclerosis FASEB J., 2016-11-08;0(0):. 2016-11-08 [PMID: 27871059] (IHC-P, Human)
• Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Int. J. Cancer, 2008-10-15;123(8):1741-50. 2008-10-15 [PMID: 18324679] (Western Blot, Human)
• Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T Interaction between the CCR5 chemokine receptors and microbial HSP70. Eur. J. Immunol., 2006-09-01;36(9):2304-14. 2006-09-01 [PMID: 16909434] (Western Blot, Human)

Review for p38 alpha Antibody (AF8691) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 10/24/2018

Summary

• Application: Western Blot
• Sample Tested: Human breast cancer cells
• Species: Human
• Lot: GIG0915111

Bioinformatics

• Gene Symbol: MAPK14
• Uniprot:
  • Q16539()