| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-597-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3919-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0340A488-100 | |||
| Alexa Fluor 546 | DDX0340A546-100 | |||
| Alexa Fluor 647 | DDX0340A647 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77231-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21753-50ul | |||
![Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IRAK4-Antibody-Western-Blot-NB500-597-img0002.jpg "Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.")
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0003.jpg "Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.")
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0006.jpg)
![Flow Cytometry: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Flow-Cytometry-NBP2-37575-img0004.jpg)
![Western Blot: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Western-Blot-NBP2-37575-img0002.jpg)
![Immunohistochemistry-Paraffin: IRAK4 Antibody (2H9) [NBP2-37575] - Analysis of human lung cancer tissues using IRAK4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-Immunohistochemistry-NBP2-37575-img0005.jpg)
![ELISA: IRAK4 Antibody (2H9) [NBP2-37575] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/IRAK4-Antibody-2H9-ELISA-NBP2-37575-img0001.jpg)
![Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Western-Blot-DDX0340P-100-img0011.jpg "Western Blot: IRAK4 Antibody (6F8) [DDX0340P-100] - Western Blot with 6F8 (Innateplateform, CLARA, Lyon)")
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - IHC staining of human FFPE tonsil section(Vermi William courtesy)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0009.jpg)
![Flow Cytometry: IRAK4 Antibody (6F8) [DDX0340P-100] - Intracellular FACS Staining of canine cell line with 6F8 (Bonnefont C, personal comm, 2011).](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Flow-Cytometry-DDX0340P-100-img0003.jpg)
![Immunohistochemistry: IRAK4 Antibody (6F8) [DDX0340P-100] - Analysis of human tonsil with 6F8 (DDX0340)](http://images.novusbio.com/fullsize/IRAK4-Antibody-6F8-Immunohistochemistry-DDX0340P-100-img0008.jpg)
![Western Blot: IRAK4 Antibody [NBP1-77231] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IRAK4-NBP1-77231 (1 ug/mL), IRAK4 (1 ug/mL), beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK4-Antibody-Western-Blot-NBP1-77231-img0009.jpg "Western Blot: IRAK4 Antibody [NBP1-77231] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: IRAK4-NBP1-77231 (1 ug/mL), IRAK4 (1 ug/mL), beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: IRAK4 Antibody [NBP1-77231] - Immunocytochemical staining of IRAK-4 protein in K562 cells using IRAK4 antibody at 10 ug/ml concentration and HRP conjugated secondary - DAB chromogen detection method.](http://images.novusbio.com/fullsize/IRAK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77231-img0007.jpg)
![Western Blot: IRAK4 Antibody [NBP1-77231] - WB detection of IRAK4 protein in HeLa whole cell lysate using IRAK4 antibody using 1:1000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions respectively.](http://images.novusbio.com/fullsize/IRAK4-Antibody-Western-Blot-NBP1-77231-img0005.jpg)
![Western Blot: IRAK4 Antibody [NBP1-77231] - Western Blot Validation in Human Cell Lines. Loading: 15 ug/ of lysates per lane. Antibodies: IRAK4 NBP1-77231 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IRAK4-Antibody-Western-Blot-NBP1-77231-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: IRAK4 Antibody - BSA Free [NBP1-77231] - Validation of IRAK4 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 Cells labeling IRAK4 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77231_rabbit-polyclonal-irak4-antibody-2711202420263214.jpg)
![Western Blot: IRAK4 Antibody (SR1922) [NBP3-21753] - Western blot analysis using NBP3-21753 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21753_rabbit-irak4-mab-sr1922-672023106230.jpg "Western Blot: IRAK4 Antibody (SR1922) [NBP3-21753] - Western blot analysis using NBP3-21753 in Jurkat cell lysate.")
![SDS-Page: IRAK4 Recombinant Protein [H00051135-P01]](http://images.novusbio.com/fullsize/IRAK4-Recombinant-Protein-SDS-Page-H00051135-P01-img0002.jpg "SDS-Page: IRAK4 Recombinant Protein [H00051135-P01]")
![Functional: Recombinant Human IRAK4 Protein [H00051135-P01]](http://images.novusbio.com/fullsize/Recombinant-Human-IRAK4-Protein-Functional-H00051135-P01-img0003.jpg)
![Western Blot: IRAK4 Antibody (JE41-77) [NBP3-32482] - Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32482_rabbit-irak4-mab-je41-77-27820241631694.jpg "Western Blot: IRAK4 Antibody (JE41-77) [NBP3-32482] - Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IRAK4 Antibody (JE41-77) [NBP3-32482] - Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-IRAK4 antibody (NBP3-32482) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32482) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32482_rabbit-irak4-mab-je41-77-298202413585559.jpg)
![Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.](http://images.novusbio.com/fullsize/IRAK4-Antibody-10H4-Western-Blot-NBP3-15153-img0002.jpg "Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.")
![Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153] - Overlay histogram showing Jurkat cells stained with IRAK4 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/IRAK4-Antibody-10H4-Flow-Cytometry-NBP3-15153-img0001.jpg)
![Western Blot: IRAK4 Overexpression Lysate (Adult Normal) [NBL1-12028] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK4.](http://images.novusbio.com/fullsize/IRAK4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12028-img0002.jpg "Western Blot: IRAK4 Overexpression Lysate (Adult Normal) [NBL1-12028] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRAK4.")
![ELISA: Human IRAK4 ELISA Kit (Colorimetric) [NBP3-39605] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39605_human-irak4-elisa-kit-colorimetric-2110202415362398.png "ELISA: Human IRAK4 ELISA Kit (Colorimetric) [NBP3-39605] - Standard Curve Reference")