IRAK4 Antibody

Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

IRAK4 Antibody Summary

• Immunogen: This antibody was developed against a mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.
• Localization: Cell Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot 1:1000 - 1:2000
• Application Notes: Immunoprecipitation: see Pacquelet et al (2007) for details.
• Control:

  NIH 3T3 Whole Cell Lysate
  IRAK4 Overexpression Lysate

Reactivity Notes

Rat reactivity reported in multiple pieces of scientific literature

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for IRAK4 Antibody

• EC 2.7.11
• EC 2.7.11.1
• interleukin-1 receptor-associated kinase 4
• IPD1
• IRAK4
• IRAK-4
• NY-REN-64
• REN64
• Renal carcinoma antigen NY-REN-64

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members (2). The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified (3). IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK4 Antibody (NB500-597)(12)

Publications using NB500-597

• Li , Wang H, Li Z et al. The inhibition of RANKL expression in fibroblasts attenuate CoCr particles induced aseptic prosthesis loosening via the MyD88-independent TLR signaling pathway. Biochem. Biophys. Res. Commun. 2018-06-25 [PMID: 29940143] (WB, Human)
• Lee YS, Park JS, Jung SM et al. Inhibition of lethal inflammatory responses through the targeting of membrane-associated Toll-like receptor 4 signaling complexes with a Smad6-derived peptide EMBO Mol Med 2015-03-12 [PMID: 25766838] (WB, Mouse)
• Valencia Pacheco GJ, Pinzon Herrera F, Cruz Lopez JJ et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013-06-30 [PMID: 24892454] (WB)
• Mirza MK, Yuan J, Gao XP et al. Caveolin-1 deficiency dampens Toll-like receptor 4 signaling through eNOS activation. Am J Pathol. 2010-05-01 [PMID: 20304961]
• Chen Yong, Liu Haizhong, Liu Zuojin et al. Blockade of inducible costimulator pathway to prevent acute rejection in rat liver transplantation. Am J Surg. 2009-08-01 [PMID: 19628063] (Rat)
• Xu Fa Liang, You Hai Bo, Li Xu Hong et al. Glycine attenuates endotoxin-induced liver injury by downregulating TLR4 signaling in Kupffer cells. Am J Surg. 2008-07-01 [PMID: 18565339] (Mouse)
• Pathak SK, Basu S, Basu KK et al. Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages. Nat Immunol. 2007-06-01 [PMID: 17486091]
• Pacquelet S, Johnson JL, Ellis BA et al. Cross-talk between IRAK-4 and the NADPH oxidase. Biochem J. 2007-05-01 [PMID: 17217339]
• Liu Zuo-jin, Yan Lu-nan, Li Shen-wei et al. Glycine blunts transplantative liver ischemia-reperfusion injury by downregulating interleukin 1 receptor associated kinase-4. Acta Pharmacol Sin. 2006-11-01 [PMID: 17049125] (WB, Rat)
• Choi KC, Lee YS, Lim S et al. Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1. Nat Immunol. 2006-10-01 [PMID: 16951688]
• Hatao F, Muroi M, Hiki N et al. Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein. J Leukoc Biol. 2004-10-01 [PMID: 15258191] (WB)
• Asehnoune K, Strassheim D, Mitra S et al. Involvement of reactive oxygen species in Toll-like receptor 4-dependent activation of NF-kappa B. J Immunol. 2004-02-15 [PMID: 14764725]

Bioinformatics

• Gene Symbol: IRAK4
• Entrez:
  • 51135(Human)
  • 266632(Mouse)
• Uniprot:
  • NP_001107654(Human)