IRAK4 Antibody

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Western Blot: IRAK4 Antibody [NB500-597] - Analysis of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using this antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

IRAK4 Antibody Summary

Immunogen
This antibody was developed against a mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRAK4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Flow Cytometry
  • Immunoprecipitation
Application Notes
Immunoprecipitation: see Pacquelet et al (2007) for details.
Control
NIH 3T3 Lysate (NB800-PC8)
IRAK4 Lysate (NBL1-12028)
Publications
Read Publications using
NB500-597 in the following applications:

  • WB
    4 publications

Reactivity Notes

Rat reactivity reported in multiple pieces of scientific literature

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for IRAK4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • interleukin-1 receptor-associated kinase 4
  • IPD1
  • IRAK4
  • IRAK-4
  • NY-REN-64
  • REN64
  • Renal carcinoma antigen NY-REN-64

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). IL-1 receptor-associated kinases (IRAKs) are important mediators in the signal transduction of Toll/IL-1 receptor (TIR) family members (2). The cytoplasmic domains of TIR proteins interact with the adapter protein, MyD88. MyD88 then recruits IRAKs (IRAK-1, -2, and M), which in turn interact with other adapter molecules, such as TRAF6 to activate NF-kB and MAPK pathways. Recently, a new member of this family, IRAK-4 has been identified (3). IRAK-4 may act as an upstream activator of IRAK-1. IRAK-4 is important for LPS activation of TLRS. Mice lacking IRAK-4 are resistant to lethal doses of LPS and are also severely impaired in their responses to viral and bacterial challenges (4, 5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IRAK4 Antibody (NB500-597)(11)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Mouse
(2)
Rat
(2)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB500-597 Applications Species
Lee YS, Park JS, Jung SM et al. Inhibition of lethal inflammatory responses through the targeting of membrane-associated Toll-like receptor 4 signaling complexes with a Smad6-derived peptide EMBO Mol Med 2015 Mar 12 [PMID: 25766838] (WB, Mouse) WB Mouse
Valencia Pacheco GJ, Pinzon Herrera F, Cruz Lopez JJ et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013 Jun 30 [PMID: 24892454] (WB) WB
Mirza MK, Yuan J, Gao XP et al. Caveolin-1 deficiency dampens Toll-like receptor 4 signaling through eNOS activation. Am J Pathol. 2010 May [PMID: 20304961]
Chen Yong, Liu Haizhong, Liu Zuojin et al. Blockade of inducible costimulator pathway to prevent acute rejection in rat liver transplantation. Am J Surg. 2009 Aug [PMID: 19628063] (Rat) Rat
Xu Fa Liang, You Hai Bo, Li Xu Hong et al. Glycine attenuates endotoxin-induced liver injury by downregulating TLR4 signaling in Kupffer cells. Am J Surg. 2008 Jul [PMID: 18565339] (Mouse) Mouse
Pathak SK, Basu S, Basu KK et al. Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages. Nat Immunol. 2007 Jun [PMID: 17486091]
Pacquelet S, Johnson JL, Ellis BA et al. Cross-talk between IRAK-4 and the NADPH oxidase. Biochem J. 2007 May 1 [PMID: 17217339]
Liu Zuo-jin, Yan Lu-nan, Li Shen-wei et al. Glycine blunts transplantative liver ischemia-reperfusion injury by downregulating interleukin 1 receptor associated kinase-4. Acta Pharmacol Sin. 2006 Nov [PMID: 17049125] (WB, Rat) WB Rat
Choi KC, Lee YS, Lim S et al. Smad6 negatively regulates interleukin 1-receptor-Toll-like receptor signaling through direct interaction with the adaptor Pellino-1. Nat Immunol. 2006 Oct [PMID: 16951688]
Hatao F, Muroi M, Hiki N et al. Prolonged Toll-like receptor stimulation leads to down-regulation of IRAK-4 protein. J Leukoc Biol. 2004 Oct [PMID: 15258191] (WB) WB
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Blogs on IRAK4.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

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Bioinformatics

Gene Symbol IRAK4
Entrez
Uniprot