Integrin alpha 8 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by ...read more
Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 8 at approximately 150 kDa (as indicated) using 10 µg/mL of ...read more
Integrin alpha 8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Integrin alpha 8 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8
Phe38-Phe1007
Accession # A28ARA8
Specificity
Detects mouse and rat Integrin alpha 8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin  alpha 8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5, recombinant mouse (rm) Integrin alpha 5, rhIntegrin alpha V and rmIntegrin  alpha V is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ITGA8
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF4076 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 8 Antibody [Unconjugated]

  • Integrin alpha 8
  • integrin alpha-8
  • integrin, alpha 8
  • ITGA8

Background

Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha 8 protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 8 Antibody (AF4076)(8)

We have publications tested in 2 confirmed species: Mouse, Canine.

We have publications tested in 6 applications: Flow, IHC, IHC Frozen, IHC Frozen, IHC-Fr, WB.


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(2)
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(7)
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Showing Publications 1 - 8 of 8.
Publications using AF4076 Applications Species
CF Hung, CL Wilson, YH Chow, LM Schnapp Role of integrin alpha8 in murine model of lung fibrosis PLoS ONE, 2018;13(5):e0197937. 2018 [PMID: 29813125] (WB,, Mouse) WB Mouse
N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development Dev. Cell, 2017;41(4):349-365.e3. 2017 [PMID: 28535371] (IHC-Fr,, Mouse) IHC-Fr Mouse
K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development Am. J. Pathol., 2017;0(0):. 2017 [PMID: 28527294] (IHC,, Mouse) IHC Mouse
SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017;0(0):. 2017 [PMID: 28219886] (Flow,, Mouse) Flow Mouse
X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage PLoS ONE, 2016;11(12):e0168343. 2016 [PMID: 27959966] (IHC,, Canine) IHC Canine
Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis. PLoS ONE, 2014;9(6):e99083. 2014 [PMID: 24915008] (IHC Frozen,, Mouse) IHC Frozen Mouse
Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013;504(7479):277-81. 2013 [PMID: 24336287] (IHC Frozen,, Mouse) IHC Frozen Mouse
Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins. J. Cell Biol., 2012;197(5):677-89. 2012 [PMID: 22613833] (WB,, Mouse) WB Mouse

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Bioinformatics

Gene Symbol ITGA8
Uniprot