Integrin alpha 8 Antibody [Unconjugated]

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 8 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8
  Phe38-Phe1007
  Accession # A28ARA8
• Specificity: Detects mouse and rat Integrin alpha 8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5, recombinant mouse (rm) Integrin alpha 5, rhIntegrin alpha V and rmIntegrin alpha V is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITGA8
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 8 Antibody [Unconjugated]

• Integrin alpha 8
• integrin alpha-8
• integrin, alpha 8
• ITGA8

Background

Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha 8 protein, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 8 Antibody (AF4076)(26)

Publications using AF4076

• Liu, J;Prahl, LS;Huang, A;Hughes, AJ; Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation bioRxiv : the preprint server for biology 2024-02-08 [PMID: 38370771] (Immunohistochemistry, Mouse)
• Viola, JM;Liu, J;Huang, A;Grindel, SH;Prahl, LS;Hughes, AJ; Rho/ROCK activity tunes cell compartment segregation and differentiation in nephron-forming niches bioRxiv : the preprint server for biology 2023-11-10 [PMID: 37986773] (Immunohistochemistry, Mouse)
• Huang B, Zeng Z, Li H et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids bioRxiv 2023-05-26 [PMID: 37293038]
• P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression Nature Cell Biology, 2023-01-30;0(0):. 2023-01-30 [PMID: 36717629] (ICC, IHC, Mouse)
• Andrew Clugston, Andrew Bodnar, Débora Malta Cerqueira, Yu Leng Phua, Alyssa Lawler, Kristy Boggs et al. Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development Genomics 2022-01-01 [PMID: 34942352]
• Sun-Sang J Sung Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli BIO-PROTOCOL 2020-07-05 [PMID: 33659333]
• Quan M Phan, Gracelyn M Fine, Lucia Salz, Gerardo G Herrera, Ben Wildman, Iwona M Driskell et al. Lef1 expression in fibroblasts maintains developmental potential in adult skin to regenerate wounds eLife 2020-09-29 [PMID: 32990218]
• S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing Cell Stem Cell, 2020-08-04;27(3):396-412.e6. 2020-08-04 [PMID: 32755548] (ICC, IHC, Mouse, Transgenic Mouse)
• Rawah Faraj, Angela Irizarry-Alfonzo, Pawan Puri Molecular characterization of nephron progenitors and their early epithelial derivative structures in the nephrogenic zone of the canine fetal kidney European Journal of Histochemistry 2019-09-23 [PMID: 31544449]
• Lori L O'Brien, Alexander N Combes, Kieran M Short, Nils O Lindström, Peter H Whitney, Luise A Cullen-McEwen et al. Wnt11 directs nephron progenitor polarity and motile behavior ultimately determining nephron endowment eLife 2018-12-05 [PMID: 30516471]
• S Oba, N Ayuzawa, M Nishimoto, W Kawarazaki, K Ueda, D Hirohama, F Kawakami-M, T Shimosawa, T Marumo, T Fujita Aberrant DNA methylation of Tgfb1 in diabetic kidney mesangial cells Sci Rep, 2018-11-05;8(1):16338. 2018-11-05 [PMID: 30397232] (ICC, Mouse)
• Chun-Chun Cheng, Ko Tsutsui, Toru Taguchi, Noriko Sanzen, Asako Nakagawa, Kisa Kakiguchi et al. Hair follicle epidermal stem cells define a niche for tactile sensation eLife 2018-10-25 [PMID: 30355452]
• CF Hung, CL Wilson, YH Chow, LM Schnapp Role of integrin alpha8 in murine model of lung fibrosis PLoS ONE, 2018-05-29;13(5):e0197937. 2018-05-29 [PMID: 29813125] (IHC, Western Blot, Mouse)
• Dominic Cosgrove, Brianna Dufek, Daniel T. Meehan, Duane Delimont, Michael Hartnett, Gina Samuelson et al. Lysyl oxidase like-2 contributes to renal fibrosis in Col4a3/Alport Mice. Kidney International 2018-08-01 [PMID: 29759420]
• Tomohiro Ogawa, Yuchang Li, Ingrid Lua, Andrea Hartner, Kinji Asahina Isolation of a unique hepatic stellate cell population expressing integrin alpha 8 from embryonic mouse livers Developmental Dynamics 2018-06-01 [PMID: 29665133]
• Ines Marek, Robert Becker, Fabian B. Fahlbusch, Carlos Menendez-Castro, Wolfgang Rascher, Christoph Daniel et al. Expression of the Alpha8 Integrin Chain Facilitates Phagocytosis by Renal Mesangial Cells Cellular Physiology and Biochemistry 2018-01-01 [PMID: 29544224]
• David A. D. Munro, Peter Hohenstein, Thomas M. Coate, Jamie A. Davies Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney Developmental Dynamics 2017-12-01 [PMID: 28929539]
• N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development Dev. Cell, 2017-05-22;41(4):349-365.e3. 2017-05-22 [PMID: 28535371] (IHC-Fr, Mouse)
• K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development Am. J. Pathol., 2017-05-17;0(0):. 2017-05-17 [PMID: 28527294] (IHC, Mouse)
• SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219886] (Flow Cytometry, IHC, Mouse)
• Sebastian Herdl, Hanna Huebner, Gudrun Volkert, Ines Marek, Carlos Menendez-Castro, Stephanie C. Noegel et al. Integrin alpha 8 Is Abundant in Human, Rat, and Mouse Trophoblasts Reproductive Sciences 2017-10-01 [PMID: 28136130]
• X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage PLoS ONE, 2016-12-13;11(12):e0168343. 2016-12-13 [PMID: 27959966] (IHC, Canine)
• Dufek Brianna, Meehan Daniel T, Delimont Duane et al. Endothelin A receptor activation on mesangial cells initiates Alport glomerular disease. Kidney International 1/1/2016 [PMID: 27165837]
• Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis. PLoS ONE, 2014-06-10;9(6):e99083. 2014-06-10 [PMID: 24915008] (IHC-Fr, Mouse)
• Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-12-12;504(7479):277-81. 2013-12-12 [PMID: 24336287] (IHC-Fr, Mouse)
• Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins. J. Cell Biol., 2012-05-21;197(5):677-89. 2012-05-21 [PMID: 22613833] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGA8
• Uniprot:
  • A28ARA8()