PDGFR alpha Antibody [Unconjugated]

Images

 
PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) ...read more
PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was ...read more
Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002. Proliferation ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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PDGFR alpha Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618
Specificity
Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha, rhPDGF R beta, and recombinant mouse PDGF R beta is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRA
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2 - 1.6 ug/mL
Reviewed Applications
Read 4 Reviews rated 5
using
AF1062 in the following applications:

Publications
Read Publications using
AF1062 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFR alpha Antibody [Unconjugated]

  • alpha-type platelet-derived growth factor receptor
  • CD140 antigen-like family member A
  • CD140a antigen
  • CD140a
  • EC 2.7.10
  • EC 2.7.10.1
  • MGC74795
  • PDGF R alpha
  • PDGFR alpha
  • PDGFR2
  • PDGFRA
  • PDGFRA/BCR fusion
  • PDGF-R-alpha
  • platelet-derived growth factor receptor, alpha polypeptide
  • rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
  • RHEPDGFRA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

  1. Betsholtz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, B/N

Publications for PDGFR alpha Antibody (AF1062)(52)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
ICC
(6)
IHC
(25)
IHC-Fr
(8)
IHC-P
(8)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(50)
Rat
(1)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using AF1062 Applications Species
MN Wosczyna, CT Konishi, EE Perez Carb, TT Wang, RA Walsh, Q Gan, MW Wagner, TA Rando Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle Cell Rep, 2019;27(7):2029-2035.e5. 2019 [PMID: 31091443] (ICC, Mouse) ICC Mouse
JM Goldstein, M Tabebordba, K Zhu, LD Wang, KA Messemer, B Peacker, S Ashrafi Ka, M Gonzalez-C, Y Shwartz, JKW Cheng, R Xiao, T Barungi, C Albright, YC Hsu, LH Vandenberg, AJ Wagers In Situ Modification of Tissue Stem and Progenitor Cell Genomes Cell Rep, 2019;27(4):1254-1264.e7. 2019 [PMID: 31018138] (IHC, Mouse) IHC Mouse
MD Hartley, T Banerji, IJ Tagge, LL Kirkemo, P Chaudhary, E Calkins, D Galipeau, MD Shokat, MJ DeBell, S Van Leuven, H Miller, G Marracci, E Pocius, T Banerji, SJ Ferrara, JM Meinig, B Emery, D Bourdette, TS Scanlan Myelin repair stimulated by CNS-selective thyroid hormone action JCI Insight, 2019;4(8):. 2019 [PMID: 30996143] (IHC, Mouse) IHC Mouse
PA Joshi, PD Waterhouse, K Kasaian, H Fang, O Gulyaeva, HS Sul, PC Boutros, R Khokha PDGFR?+ stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland Nat Commun, 2019;10(1):1760. 2019 [PMID: 30988300] (IHC, Mouse) IHC Mouse
X Han, Y Wang, W Pu, X Huang, L Qiu, Y Li, W Yu, H Zhao, X Liu, L He, L Zhang, Y Ji, J Lu, KO Lui, B Zhou Lineage Tracing Reveals the Bipotency of SOX9+ Hepatocytes during Liver Regeneration Stem Cell Reports, 2019;12(3):624-638. 2019 [PMID: 30773487] (IHC, Mouse) IHC Mouse
CF Guerrero-J, PH Dedhia, S Jin, R Ruiz-Vega, D Ma, Y Liu, K Yamaga, O Shestova, DL Gay, Z Yang, K Kessenbroc, Q Nie, WS Pear, G Cotsarelis, MV Plikus Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds Nat Commun, 2019;10(1):650. 2019 [PMID: 30737373] (IHC-P, Mouse) IHC-P Mouse
L Lukjanenko, S Karaz, P Stuelsatz, U Gurriaran-, J Michaud, G Dammone, F Sizzano, O Mashinchia, S Ancel, E Migliavacc, S Liot, G Jacot, S Metairon, F Raymond, P Descombes, A Palini, B Chazaud, MA Rudnicki, CF Bentzinger, JN Feige Aging Disrupts Muscle Stem Cell Function by Impairing Matricellular WISP1 Secretion from Fibro-Adipogenic Progenitors Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30686765] (IHC, Mouse) IHC Mouse
D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage Nat. Immunol., 2019;20(2):163-172. 2019 [PMID: 30643263] (IHC-Fr, Mouse) IHC-Fr Mouse
K Kretzschma, Y Post, M Bannier-Hé, A Mattiotti, J Drost, O Basak, VSW Li, M van den Bo, QD Gunst, D Versteeg, L Kooijman, S van der El, JH van Es, E van Rooij, MJB van den Ho, H Clevers Profiling proliferative cells and their progeny in damaged murine hearts Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30530645] (IHC-Fr, Mouse) IHC-Fr Mouse
S Oishi, D Harkins, ND Kurniawan, M Kasherman, L Harris, O Zalucki, RM Gronostajs, THJ Burne, M Piper Heterozygosity for Nuclear Factor One X in mice models features of Malan syndrome EBioMedicine, 2018;0(0):. 2018 [PMID: 30503862] (IHC, Mouse) IHC Mouse
Show All 52 Publications.

Reviews for PDGFR alpha Antibody (AF1062) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF1062:
Filter by Applications
ICC
(2)
IHC-Fr
(2)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry PDGFR alpha AF1062
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reviewed by:
Kevin Thorburn
ICC Mouse 07/12/2019
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Summary

ApplicationImmunocytochemistry
Sample TestedPrimary Oligodendrocyte Precursor Cells
SpeciesMouse
LotHMQ0217091

Comments

CommentsCells were obtained via immunopanning Foo et al. 2011 and grown in a defined, serum-free media for 3 days. Cells were fixed with 4% PFA for 10 mins, washed 3 times with D-PBS containing Ca2+ and Mg2+, blocked in D-PBS containing 10% donkey serum for 30min and stained overnight with PDGFR alpha antibody final concentration 0.4 micrograms/mL in D-PBS containing 2% donkey serum and 2% BSA. The next day, cells were washed with D-PBS three times and incubated with Alexa Fluor 488 conjugated Jackson Immuno donkey anti-goat IgG H+L for 30 mins at room temperature. Cells were then washed 3 times with D-PBS and visualized.
Immunohistochemistry-Frozen PDGFR alpha AF1062
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reviewed by:
Jun Cai
IHC-Fr Mouse 03/15/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord
SpeciesMouse
LotHMQ0217031
Immunocytochemistry PDGFR alpha AF1062
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reviewed by:
Xing Fu
ICC Mouse 08/12/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedmouse skeletal muscle primary cells,mouse skeletal muscle-derived cells,mouse skeletal muscle cells
SpeciesMouse
LotHMQ0217111
Immunohistochemistry-Frozen PDGFR alpha AF1062
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reviewed by:
Michelle Tallquist
IHC-Fr Mouse 01/07/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult Mouse Lung Tissue,Adult heart
SpeciesMouse
LotHMQ0215021

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FAQs for PDGFR alpha Antibody (AF1062) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 FAB1062G
Allophycocyanin FAB1062A
Biotin BAF1062
Phycoerythrin FAB1062P

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Recent Reviews

5
5
4
4
0
3
0
2
0
1
0

Kevin Thorburn
07/12/2019
Application: ICC
Species: Mouse

Jun Cai
03/15/2019
Application: IHC-Fr
Species: Mouse

Xing Fu
08/12/2018
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol PDGFRA
Uniprot