PDGFR alpha Antibody [Unconjugated]

Western blot shows lysates of mouse uterus tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PDGF R alpha at approximately 160-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

PDGF R alpha was detected in immersion fixed frozen sections of mouse embryo using 15 µg/mL Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Human PDGF‑AA (Catalog # 221-AA) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002. Proliferation elicited by Recombinant Human PDGF‑AA (250 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse PDGF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1062). The ND50 is typically 0.2-1.6 µg/mL.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDGFR alpha Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha
  Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala)
  Accession # P26618
• Specificity: Detects mouse PDGF R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PDGF R alpha , rhPDGF R beta , and recombinant mouse PDGF R beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFRA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.2 - 1.6 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGFR alpha Antibody [Unconjugated]

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha  and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta  hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta  homo-dimerization is induced only by PDGF-BB and -DD (1-4). Both PDGF R alpha  and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha  and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets, and megakaryocytes) or only the  beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells, and macrophages) (1, 2). Recombinant mouse and human soluble PDGF R beta  bind PDGF with high affinity and are potent PDGF antagonists.

1. Betsholtz, C. et al. (2001) BioEssays 23:494.
2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (AF1062)(135)

Publications using AF1062

• Waldhauser, V;Baroti, T;Fröb, F;Wegner, M; PBAF Subunit Pbrm1 Selectively Influences the Transition from Progenitors to Pre-Myelinating Cells during Oligodendrocyte Development Cells 2023-06-06 [PMID: 37371026] (IHC, Mouse)
• Norris, AM;Appu, AB;Johnson, CD;Zhou, LY;McKellar, DW;Renault, MA;Hammers, D;Cosgrove, BD;Kopinke, D; Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration Nature communications 2023-06-24 [PMID: 37355632] (IHC, Mouse)
• Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ; Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration Cell reports 2023-06-16 [PMID: 37330908]
• Alber, AB;Marquez, HA;Ma, L;Kwong, G;Thapa, BR;Villacorta-Martin, C;Lindstrom-Vautrin, J;Bawa, P;Wang, F;Luo, Y;Ikonomou, L;Shi, W;Kotton, DN; Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme Nature communications 2023-06-13 [PMID: 37311756] (IHC, Transgenic Mouse)
• Kalgudde Gopal, S;Dai, R;Stefanska, AM;Ansari, M;Zhao, J;Ramesh, P;Bagnoli, JW;Correa-Gallegos, D;Lin, Y;Christ, S;Angelidis, I;Lupperger, V;Marr, C;Davies, LC;Enard, W;Machens, HG;Schiller, HB;Jiang, D;Rinkevich, Y; Wound infiltrating adipocytes are not myofibroblasts Nature communications 2023-05-25 [PMID: 37230982] (IHC, Mouse)
• Wang, K;Papadopoulos, N;Hamidi, A;Lennartsson, J;Heldin, CH; SUMOylation of PDGF receptor ? affects signaling via PLC? and STAT3, and cell proliferation BMC molecular and cell biology 2023-05-16 [PMID: 37193980] (Immunoprecipitation, IHC, Western Blot, African Green Monkey, Human)
• Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ; Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury Cell reports 2023-05-06 [PMID: 37149868] (IHC, Transgenic Mouse)
• A Kolaj, SK Zahr, BS Wang, T Krawec, H Kazan, G Yang, DR Kaplan, FD Miller The P-body protein 4E-T represses translation to regulate the balance between cell genesis and establishment of the postnatal NSC pool Cell Reports, 2023-03-15;42(3):112242. 2023-03-15 [PMID: 36924490] (ICC, Mouse)
• K Hoeft, GJL Schaefer, H Kim, D Schumacher, T Bleckwehl, Q Long, BM Klinkhamme, F Peisker, L Koch, J Nagai, M Halder, S Ziegler, E Liehn, C Kuppe, J Kranz, S Menzel, I Costa, A Wahida, P Boor, RK Schneider, S Hayat, R Kramann Platelet-instructed SPP1+ macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent manner Cell Reports, 2023-02-17;42(2):112131. 2023-02-17 [PMID: 36807143] (IHC, Mouse)
• ME Mohr, S Li, AM Trouten, RA Stairley, PL Roddy, C Liu, M Zhang, HM Sucov, G Tao Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury bioRxiv : the preprint server for biology, 2023-02-08;0(0):. 2023-02-08 [PMID: 36798323] (IHC, Mouse)
• E Manieri, G Tie, D Seruggia, S Madha, A Maglieri, K Huang, Y Fujiwara, K Zhang, S Orkin, R He, N McCarthy, RA Shivdasani Structure, signals, and cellular elements of the mouse gastric mesenchymal niche bioRxiv : the preprint server for biology, 2023-02-24;0(0):. 2023-02-24 [PMID: 36798304] (IHC, Mouse)
• NL Jacobsen, AB Morton, SS Segal Angiogenesis precedes myogenesis during regeneration following biopsy injury of skeletal muscle Skeletal Muscle, 2023-02-14;13(1):3. 2023-02-14 [PMID: 36788624] (IHC, Mouse)
• Z Li, K Chen, Q Shao, H Lu, X Zhang, Y Pu, X Sun, H He, L Cao Nanoparticulate MgH2 ameliorates anxiety/depression-like behaviors in a mouse model of multiple sclerosis by regulating microglial polarization and oxidative stress Journal of Neuroinflammation, 2023-01-30;20(1):16. 2023-01-30 [PMID: 36710351] (ICC, IHC, Mouse)
• L Legouez, B Le Dieu-Lu, S Feillet, G Riou, M Yeddou, T Plouchart, N Dourmap, MA Le Ray, S Marret, BJ Gonzalez, C Cleren Effects of MgSO4 Alone or Associated with 4-PBA on Behavior and White Matter Integrity in a Mouse Model of Cerebral Palsy: A Sex- and Time-Dependent Study International Journal of Molecular Sciences, 2022-12-15;23(24):. 2022-12-15 [PMID: 36555591] (IHC, Mouse)
• CA Pagani, AC Bancroft, RJ Tower, N Livingston, Y Sun, JY Hong, RN Kent, AL Strong, JH Nunez, JMR Medrano, N Patel, BA Nanes, KM Dean, Z Li, C Ge, BM Baker, AW James, SJ Weiss, RT Franceschi, B Levi Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization Science Advances, 2022-12-21;8(51):eabq6152. 2022-12-21 [PMID: 36542719] (IHC/IF, Mouse)
• X Hong, Y Jian, S Ding, J Zhou, X Zheng, H Zhang, B Zhou, C Zhuang, J Wan, X Tong Kir4.1 channel activation in NG2 glia contributes to remyelination in ischemic stroke EBioMedicine, 2022-12-15;87(0):104406. 2022-12-15 [PMID: 36527899] (IHC, Mouse)
• P Cattaneo, MGB Hayes, N Baumgarten, D Hecker, S Peruzzo, GS Aslan, P Kunderfran, V Larcher, L Zhang, R Contu, G Fonseca, S Spinozzi, J Chen, G Condorelli, S Dimmeler, MH Schulz, S Heinz, N Guimarães-, SM Evans DOT1L regulates chamber-specific transcriptional networks during cardiogenesis and mediates postnatal cell cycle withdrawal Nature Communications, 2022-12-02;13(1):7444. 2022-12-02 [PMID: 36460641] (IHC/IF, Mouse)
• K Ono, H Gotoh, T Nomura, T Morita, O Baba, M Matsumoto, S Saitoh, N Ohno Ultrastructural characteristics of oligodendrocyte precursor cells in the early postnatal mouse optic nerve observed by serial block-face scanning electron microscopy PLoS ONE, 2022-12-01;17(12):e0278118. 2022-12-01 [PMID: 36454994] (IHC, Mouse)
• C Bernhardt, E Sock, F Fröb, S Hillgärtne, M Nemer, M Wegner KLF9 and KLF13 transcription factors boost myelin gene expression in oligodendrocytes as partners of SOX10 and MYRF Nucleic Acids Research, 2022-11-11;0(0):. 2022-11-11 [PMID: 36318265] (IHC, Mouse)
• T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging Nature Neuroscience, 2022-10-24;25(11):1446-1457. 2022-10-24 [PMID: 36280798] (IHC, Mouse)
• M Sobecki, J Chen, E Krzywinska, S Nagarajan, Z Fan, E Nelius, JM Monné Rodr, F Seehusen, A Hussein, G Moschini, EY Hajam, R Kiran, D Gotthardt, J Debbache, C Badoual, T Sato, T Isagawa, N Takeda, C Tanchot, E Tartour, A Weber, S Werner, J Loffing, L Sommer, V Sexl, C Münz, C Feghali-Bo, E Pachera, O Distler, J Snedeker, C Jamora, C Stockmann Vaccination-based immunotherapy to target profibrotic cells in liver and lung Cell Stem Cell, 2022-09-15;0(0):. 2022-09-15 [PMID: 36113462] (Flow Cytometry, IHC, Mouse)
• RM Beiter, C Rivet-Noor, AR Merchak, R Bai, DM Johanson, E Slogar, K Sol-Church, CC Overall, A Gaultier Evidence for oligodendrocyte progenitor cell heterogeneity in the adult mouse brain Scientific Reports, 2022-07-28;12(1):12921. 2022-07-28 [PMID: 35902669] (IHC, Mouse)
• SE Ha, B Jin, BG Jorgensen, H Zogg, L Wei, R Singh, C Park, M Kurahashi, S Kim, G Baek, SM Poudrier, MY Lee, KM Sanders, S Ro Transcriptome profiling of subepithelial PDGFRalpha cells in colonic mucosa reveals several cell-selective markers PLoS ONE, 2022-05-13;17(5):e0261743. 2022-05-13 [PMID: 35560163] (IHC, Mouse)
• JM Jacob, SE Di Carlo, I Stzepourgi, A Lepelletie, PD Ndiaye, H Varet, R Legendre, E Kornobis, A Benabid, G Nigro, L Peduto PDGFRalpha-induced stromal maturation is required to restrain postnatal intestinal epithelial stemness and promote defense mechanisms Cell Stem Cell, 2022-05-05;29(5):856-868.e5. 2022-05-05 [PMID: 35523143] (Flow Cytometry, Mouse)
• S Ghorbani, E Jelinek, R Jain, B Buehner, C Li, BM Lozinski, S Sarkar, DK Kaushik, Y Dong, TN Wight, S Karimi-Abd, GJ Schenk, EM Strijbis, J Geurts, P Zhang, CC Ling, VW Yong Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination Nature Communications, 2022-05-04;13(1):2445. 2022-05-04 [PMID: 35508608] (IHC, Mouse)
• JK Knowles, H Xu, C Soane, A Batra, T Saucedo, E Frost, LT Tam, D Fraga, L Ni, K Villar, S Talmi, JR Huguenard, M Monje Maladaptive myelination promotes generalized epilepsy progression Nature Neuroscience, 2022-05-02;25(5):596-606. 2022-05-02 [PMID: 35501379] (IHC, Rat)
• Y Zhang, E Manouchehr, M Herre, J Cedervall, Q Qiao, Z Miao, A Hamidi, L Hellman, M Kamali-Mog, AK Olsson Platelet-Derived PDGFB Promotes Recruitment of Cancer-Associated Fibroblasts, Deposition of Extracellular Matrix and Tgfbeta Signaling in the Tumor Microenvironment Cancers, 2022-04-12;14(8):. 2022-04-12 [PMID: 35454853] (Mouse)
• T Potluri, MJ Taylor, JJ Stulberg, RL Lieber, H Zhao, SE Bulun An estrogen-sensitive fibroblast population drives abdominal muscle fibrosis in an inguinal hernia mouse model JCI Insight, 2022-04-19;7(9):. 2022-04-19 [PMID: 35439171] (ICC, Mouse)
• S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation Journal of Cell Science, 2022-04-12;0(0):. 2022-04-12 [PMID: 35293576] (IHC, Mouse)
• XR Ma, X Zhu, Y Xiao, HM Gu, SS Zheng, L Li, F Wang, ZJ Dong, DX Wang, Y Wu, C Yang, W Jiang, K Yao, Y Yin, Y Zhang, C Peng, L Gao, Z Meng, Z Hu, C Liu, L Li, HZ Chen, Y Shu, Z Ju, JW Zhao Restoring nuclear entry of Sirtuin 2 in oligodendrocyte progenitor cells promotes remyelination during ageing Nature Communications, 2022-03-09;13(1):1225. 2022-03-09 [PMID: 35264567] (IF, Mouse)
• A Fernández-, P Lu, AC Geraghty, E Song, MH Lee, J Wood, B Yalç?n, KR Taylor, S Dutton, L Acosta-Alv, L Ni, D Contreras-, JR Gehlhausen, J Klein, C Lucas, T Mao, J Silva, MA Peña-Herná, A Tabachniko, T Takahashi, L Tabacof, J Tosto-Manc, E Breyman, A Kontorovic, D McCarthy, M Quezado, M Hefti, D Perl, R Folkerth, D Putrino, A Nath, A Iwasaki, M Monje Mild respiratory SARS-CoV-2 infection can cause multi-lineage cellular dysregulation and myelin loss in the brain bioRxiv : the preprint server for biology, 2022-01-10;0(0):. 2022-01-10 [PMID: 35043113] (IHC, Mouse)
• V Reyes Nico, JM Allaire, AB Alfonso, D Pupo Gómez, V Pomerleau, V Giroux, F Boudreau, N Perreault Altered Mucus Barrier Integrity and Increased Susceptibility to Colitis in Mice upon Loss of Telocyte Bone Morphogenetic Protein Signalling Cells, 2021-10-29;10(11):. 2021-10-29 [PMID: 34831177] (IHC, Mouse)
• YJ Li, JP Ao, X Huang, HL Lu, HY Fu, NN Song, WX Xu, J Chen Involvement of PAR2 in platelet-derived growth factor receptor-alpha-positive cell proliferation in the colon of diabetic mice Physiological Reports, 2021-11-01;9(21):e15099. 2021-11-01 [PMID: 34755491] (IHC, Mouse)
• F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021-10-20;599(7883):125-130. 2021-10-20 [PMID: 34671159] (IHC, Mouse)
• N Cohen, H Massalha, S Ben-Moshe, A Egozi, M Rozenberg, K Bahar Halp, S Itzkovitz Spatial gene expression maps of the intestinal lymphoid follicle and associated epithelium identify zonated expression programs PloS Biology, 2021-10-11;19(10):e3001214. 2021-10-11 [PMID: 34634036] (IF, Mouse)
• Y Wang, Y Zhang, S Zhang, B Kim, VL Hull, J Xu, P Prabhu, M Gregory, V Martinez-C, X Zhan, W Deng, F Guo PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination Cell Reports, 2021-10-05;37(1):109695. 2021-10-05 [PMID: 34610310] (IHC, Mouse)
• X Zhang, Y Liu, X Hong, X Li, CK Meshul, C Moore, Y Yang, Y Han, WG Li, X Qi, H Lou, S Duan, TL Xu, X Tong NG2 glia-derived GABA release tunes inhibitory synapses and contributes to stress-induced anxiety Nature Communications, 2021-09-30;12(1):5740. 2021-09-30 [PMID: 34593806] (IHC, Mouse)
• H Shin, HD Kawai Sensitive timing of undifferentiation in oligodendrocyte progenitor cells and their enhanced maturation in primary visual cortex of binocularly enucleated mice PLoS ONE, 2021-09-17;16(9):e0257395. 2021-09-17 [PMID: 34534256] (IHC, Mouse)
• Y Takanezawa, S Tanabe, D Kato, R Ozeki, M Komoda, T Suzuki, H Baba, R Muramatsu Microglial ASD-related genes are involved in oligodendrocyte differentiation Scientific Reports, 2021-09-08;11(1):17825. 2021-09-08 [PMID: 34497307] (ICC, IHC, Mouse)
• K Lee, SO Park, PC Choi, SB Ryoo, H Lee, LE Peri, T Zhou, RD Corrigan, AC Yanez, SB Moon, BA Perrino, KM Sanders, SD Koh Molecular and functional characterization of detrusor PDGFRalpha positive cells in spinal cord injury-induced detrusor overactivity Scientific Reports, 2021-08-11;11(1):16268. 2021-08-11 [PMID: 34381120] (IHC, Mouse)
• L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation Cell Stem Cell, 2021-08-02;0(0):. 2021-08-02 [PMID: 34343491] (IHC, Mouse)
• I De, V Maklakova, S Litscher, MM Boyd, LC Klemm, Z Wang, C Kendziorsk, LS Collier Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages Journal of Neuroinflammation, 2021-07-19;18(1):162. 2021-07-19 [PMID: 34281564] (IHC, Mouse)
• KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams Regional specialization and fate specification of bone stromal cells in skeletal development Cell Reports, 2021-07-13;36(2):109352. 2021-07-13 [PMID: 34260921] (IHC, Mouse)
• SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality Cell Reports, 2021-06-08;35(10):109223. 2021-06-08 [PMID: 34107247] (IHC, Mouse)
• A Waisman, AM Norris, M Elías Cost, D Kopinke Automatic and unbiased segmentation and quantification of myofibers in skeletal muscle Scientific Reports, 2021-06-03;11(1):11793. 2021-06-03 [PMID: 34083673] (IHC, Mouse)
• F Pfeiffer, A Sherafat, A Nishiyama The Impact of Fixation on the Detection of Oligodendrocyte Precursor Cell Morphology and Vascular Associations Cells, 2021-05-24;10(6):. 2021-05-24 [PMID: 34073801] (IHC, Mouse)
• Y Pan, JD Hysinger, T Barron, NF Schindler, O Cobb, X Guo, B Yalç?n, C Anastasaki, SB Mulinyawe, A Ponnuswami, S Scheaffer, Y Ma, KC Chang, X Xia, JA Toonen, JJ Lennon, EM Gibson, JR Huguenard, LM Liau, JL Goldberg, M Monje, DH Gutmann NF1 mutation drives neuronal�activity-dependent initiation of optic glioma Nature, 2021-05-26;594(7862):277-282. 2021-05-26 [PMID: 34040258] (ICC, IHC, Mouse)
• O Contreras, A Córdova-Ca, E Brandan PDGF-PDGFR network differentially regulates the fate, migration, proliferation, and cell cycle progression of myogenic cells Cellular Signalling, 2021-05-08;84(0):110036. 2021-05-08 [PMID: 33971280] (ICC/IF, Western Blot, Mouse)
• MN Wosczyna, EE Perez Carb, MW Wagner, S Paredes, CT Konishi, L Liu, TT Wang, RA Walsh, Q Gan, CS Morrissey, TA Rando Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells Cell Stem Cell, 2021-05-03;0(0):. 2021-05-03 [PMID: 33945794] (Flow Cytometry, IHC, Mouse)
• K Kimura, K Ramirez, TAV Nguyen, Y Yamashiro, A Sada, H Yanagisawa Contribution of PDGFR&amp;alpha-positive cells in maintenance and injury responses in mouse large vessels Scientific Reports, 2021-04-21;11(1):8683. 2021-04-21 [PMID: 33883668] (IHC, Mouse)
• W Mu, S Li, J Xu, X Guo, H Wu, Z Chen, L Qiao, G Helfer, F Lu, C Liu, QF Wu Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice Nature Communications, 2021-04-16;12(1):2288. 2021-04-16 [PMID: 33863883] (IHC, Mouse)
• L Mészáros, MJ Riemenschn, H Gassner, F Marxreiter, S von Hörste, A Hoffmann, J Winkler Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophy Acta neuropathologica communications, 2021-04-14;9(1):68. 2021-04-14 [PMID: 33853667] (IHC, Mouse)
• T Hiroshige, KI Uemura, S Hirashima, K Hino, A Togo, K Ohta, T Igawa, KI Nakamura Identification of PDGFR&amp;alpha-positive interstitial cells in the distal segment of the murine vas deferens Scientific Reports, 2021-04-06;11(1):7553. 2021-04-06 [PMID: 33824385] (IHC, Mouse)
• K Moore, D Fulmer, L Guo, N Koren, J Glover, R Moore, C Gensemer, T Beck, J Morningsta, R Stairley, RA Norris PDGFR&amp;alpha: Expression and Function during Mitral Valve Morphogenesis Journal of cardiovascular development and disease, 2021-03-13;8(3):. 2021-03-13 [PMID: 33805717] (IHC, Mouse)
• S Choi, B Zhang, S Ma, M Gonzalez-C, D Stein, X Jin, ST Kim, YL Kang, A Besnard, A Rezza, L Grisanti, JD Buenrostro, M Rendl, M Nahrendorf, A Sahay, YC Hsu Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence Nature, 2021-03-31;0(0):. 2021-03-31 [PMID: 33790465] (IHC, Mouse)
• D Yamakawa, D Katoh, K Kasahara, T Shiromizu, M Matsuyama, C Matsuda, Y Maeno, M Watanabe, Y Nishimura, M Inagaki Primary cilia-dependent lipid raft/caveolin dynamics regulate adipogenesis Cell Reports, 2021-03-09;34(10):108817. 2021-03-09 [PMID: 33691104] (ICC, IHC, Mouse)
• LL Boshans, H Soh, WM Wood, TM Nolan, II Mandoiu, Y Yanagawa, AV Tzingounis, A Nishiyama Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2 Scientific Reports, 2021-02-11;11(1):3552. 2021-02-11 [PMID: 33574458] (IHC, Mouse)
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• Su EJ, Fredriksson L, Geyer M, Folestad E, Cale J, Andrae J, Gao Y, Pietras K, Mann K, Yepes M, Strickland DK, Betsholtz C, Eriksson U, Lawrence DA Activation of PDGF-CC by tissue plasminogen activator impairs blood-brain barrier integrity during ischemic stroke. Nat. Med., 2008-06-22;14(7):731-7. 2008-06-22 [PMID: 18568034] (Western Blot, Mouse)
• Makar TK, Trisler D, Bever CT, Goolsby JE, Sura KT, Balasubramanian S, Sultana S, Patel N, Ford D, Singh IS, Gupta A, Valenzuela RM, Dhib-Jalbut S Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis. J. Neuroimmunol., 2008-05-08;196(1):67-81. 2008-05-08 [PMID: 18471898] (IHC-P, Mouse)
• Luyt K, Varadi A, Durant CF, Molnar E Oligodendroglial metabotropic glutamate receptors are developmentally regulated and involved in the prevention of apoptosis. J. Neurochem., 2006-07-06;99(2):641-56. 2006-07-06 [PMID: 16836654] (ICC, Mouse)
• Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma. Clin. Cancer Res., 2006-05-01;12(9):2676-88. 2006-05-01 [PMID: 16675559] (IHC-P, Human)

Reviews for PDGFR alpha Antibody (AF1062) (4)

Average Rating: 5

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Mouse.

reviewed by: Kevin Thorburn ICC Mouse 07/12/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary Oligodendrocyte Precursor Cells
• Species: Mouse
• Lot: HMQ0217091

Comments

• Comments: Cells were obtained via immunopanning Foo et al. 2011 and grown in a defined, serum-free media for 3 days. Cells were fixed with 4% PFA for 10 mins, washed 3 times with D-PBS containing Ca2+ and Mg2+, blocked in D-PBS containing 10% donkey serum for 30min and stained overnight with PDGFR alpha antibody final concentration 0.4 micrograms/mL in D-PBS containing 2% donkey serum and 2% BSA. The next day, cells were washed with D-PBS three times and incubated with Alexa Fluor 488 conjugated Jackson Immuno donkey anti-goat IgG H+L for 30 mins at room temperature. Cells were then washed 3 times with D-PBS and visualized.

reviewed by: Jun Cai IHC-Fr Mouse 03/15/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: HMQ0217031

reviewed by: Xing Fu ICC Mouse 08/12/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse skeletal muscle cells,mouse skeletal muscle primary cells,mouse skeletal muscle-derived cells
• Species: Mouse
• Lot: HMQ0217111

reviewed by: Michelle Tallquist IHC-Fr Mouse 01/07/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Adult heart,Adult Mouse Lung Tissue
• Species: Mouse
• Lot: HMQ0215021

Bioinformatics

• Gene Symbol: PDGFRA
• Uniprot:
  • P26618()