Mer Antibody [Unconjugated]

Images

 
J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, ...read more
Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed ...read more
Mer mRNA was detected in formalin-fixed paraffin-embedded tissue sections of mouse kidney probed with ACD RNAScope® Probe (Catalog # 441241) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, Dual ISH-IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Mer Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Specificity
Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MERTK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 1-15 ug/mL
Control
Mer Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
AF591 in the following applications:

Publications
Read Publications using
AF591 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mer Antibody [Unconjugated]

  • c-Eyk
  • c-mer proto-oncogene tyrosine kinase
  • C-mer
  • EC 2.7.10
  • EC 2.7.10.1
  • MER receptor tyrosine kinase
  • Mer
  • MerTK
  • MGC133349
  • Receptor tyrosine kinase MerTK
  • RP38Proto-oncogene c-Mer
  • STK kinase
  • tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Mer Antibody (AF591)(32)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: Cell Culture, Flow Cytometry, ICC, IF, IHC-Fr, IHC-P, Immunoprecipitation, In Vivo, Neutralization, Western Blot, in vitro.


Filter By Application
Cell Culture
(1)
Flow Cytometry
(6)
ICC
(1)
IF
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(4)
In Vivo
(1)
Neutralization
(2)
Western Blot
(12)
in vitro
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(28)
Rat
(2)
All Species
Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using AF591 Applications Species
B Moon, J Lee, SA Lee, C Min, H Moon, D Kim, S Yang, H Moon, J Jeon, YE Joo, D Park Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis Cells, 2020;9(7):. 2020 [PMID: 32640697] (ICC, Human) ICC Human
EC Damisah, RA Hill, A Rai, F Chen, CV Rothlin, S Ghosh, J Grutzendle Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo Sci Adv, 2020;6(26):eaba3239. 2020 [PMID: 32637606] (IF, Mouse) IF Mouse
A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31689560] (Cell Culture, Mouse) Cell Culture Mouse
RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer Cancer Res., 2019;0(0):. 2019 [PMID: 30877108] (Flow Cytometry, Mouse) Flow Cytometry Mouse
D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage Nat. Immunol., 2019;20(2):163-172. 2019 [PMID: 30643263] (IHC-Fr, Mouse) IHC-Fr Mouse
DG Thomas, AC Doran, P Fotakis, M Westerterp, P Antonson, H Jiang, XC Jiang, JÅ Gustafsson, I Tabas, AR Tall LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux Cell Rep, 2018;25(13):3774-3785.e4. 2018 [PMID: 30590048]
W Du, H Huang, N Sorrelle, RA Brekken Sitravatinib potentiates immune checkpoint blockade in refractory cancer models JCI Insight, 2018;3(21):. 2018 [PMID: 30385724] (Western Blot, Mouse) Western Blot Mouse
CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models Front Immunol, 2018;9(0):742. 2018 [PMID: 29706963] (In Vivo, Mouse) In Vivo Mouse
A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28874532] (Western Blot, Mouse) Western Blot Mouse
A Canfrán-Du, N Rotllan, X Zhang, M Fernández-, C Ramírez-Hi, E Araldi, L Daimiel, R Busto, C Fernández-, Y Suárez Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28674080] (Western Blot, Mouse) Western Blot Mouse
Show All 32 Publications.

Reviews for Mer Antibody (AF591) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF591:
Filter by Applications
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Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 Mer AF591
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4
reviewed by:
Fuyi Chen
Mouse 03/16/2018
View

Summary

Sample TestedAdult mouse brain
SpeciesMouse
LotDGS031611B

Comments

Commentsantigen retrieval for 15 mins. 1:250 dilution.
  4
reviewed by:
Juwen DuBois
Mouse 04/03/2017
View

Summary

Sample TestedAdult mouse brain, brain and spinal cord
SpeciesMouse
LotDGS0416031

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FAQs for Mer Antibody (AF591) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 FAB591G
Alexa Fluor 647 FAB591R
Biotin BAF591
Phycoerythrin FAB591P

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Fuyi Chen
03/16/2018
Application:
Species: Mouse

 
Juwen DuBois
04/03/2017
Application:
Species: Mouse

Bioinformatics

Gene Symbol MERTK
Uniprot