Mer Antibody [Unconjugated]

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Mer was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Mer mRNA was detected in formalin-fixed paraffin-embedded tissue sections of mouse kidney probed with ACD RNAScope® Probe (Catalog # 441241) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Mouse Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF591) at 1 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

• Reactivity: Mu
• Applications: Flow, IHC, CyTOF-ready, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mer Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
  Glu23-Phe498
  Accession # Q60805
• Specificity: Detects mouse Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human Mer is observed and less than 1% cross-reactivity with recombinant mouse (rm) Axl and rmDtk is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MERTK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 1-15 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 1-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mer Antibody [Unconjugated]

• c-Eyk
• c-mer proto-oncogene tyrosine kinase
• C-mer
• EC 2.7.10
• EC 2.7.10.1
• MER receptor tyrosine kinase
• Mer
• MerTK
• MGC133349
• Receptor tyrosine kinase MerTK
• RP38Proto-oncogene c-Mer
• STK kinase
• tyrosine-protein kinase Mer

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1, 2).

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mer Antibody (AF591)(43)

Publications using AF591

• H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022-08-04;0(0):. 2022-08-04 [PMID: 35927581] (Flow Cytometry, Mouse)
• L Zheng, J Jia, Y Chen, R Liu, R Cao, M Duan, M Zhang, Y Xu Pentoxifylline alleviates ischemic white matter injury through up-regulating Mertk-mediated myelin clearance Journal of Neuroinflammation, 2022-05-31;19(1):128. 2022-05-31 [PMID: 35642056] (IHC, Mouse)
• X Huang, C Liu, N Kong, Y Xiao, A Yurdagul, I Tabas, W Tao Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages Nature Protocols, 2022-02-04;0(0):. 2022-02-04 [PMID: 35121853] (IHC, Mouse)
• Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke Microglia use TAM receptors to detect and engulf amyloid &beta plaques Nature Immunology, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859405] (IHC, Mouse)
• NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3 Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7. 2021-02-01 [PMID: 33538769] (Western Blot, Mouse)
• JM Rosin, CM Marsters, F Malik, R Far, L Adnani, C Schuurmans, QJ Pittman, DM Kurrasch Embryonic Microglia Interact with Hypothalamic Radial Glia during Development and Upregulate the TAM Receptors MERTK and AXL following an Insult Cell Reports, 2021-01-05;34(1):108587. 2021-01-05 [PMID: 33406432] (IHC, Mouse)
• H Wu, J Zheng, S Xu, Y Fang, Y Wu, J Zeng, A Shao, L Shi, J Lu, S Mei, X Wang, X Guo, Y Wang, Z Zhao, J Zhang Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury Journal of Neuroinflammation, 2021-01-05;18(1):2. 2021-01-05 [PMID: 33402181] (IHC, Mouse)
• C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain Nature Communications, 2020-11-30;11(1):6133. 2020-11-30 [PMID: 33257673] (Western Blot, Mouse)
• CY Lu, K Santosa, A Jablonka-S, B Vannucci, A Fuchs, I Turnbull, D Pan, M Wood, AK Snyder-War Macrophage-derived vascular endothelial growth factor-A is integral to neuromuscular junction reinnervation after nerve injury J Neurosci, 2020-11-06;0(0):. 2020-11-06 [PMID: 33158964] (Flow Cytometry, Mouse)
• JC DuBois, AK Ray, P Davies, B Shafit-Zag Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis J Neuroinflammation, 2020-10-29;17(1):324. 2020-10-29 [PMID: 33121494] (Functional Assay, Mouse)
• B Moon, J Lee, SA Lee, C Min, H Moon, D Kim, S Yang, H Moon, J Jeon, YE Joo, D Park Mertk Interacts with Tim-4 to Enhance Tim-4-Mediated Efferocytosis Cells, 2020-07-06;9(7):. 2020-07-06 [PMID: 32640697] (ICC, Human)
• EC Damisah, RA Hill, A Rai, F Chen, CV Rothlin, S Ghosh, J Grutzendle Astrocytes and microglia play orchestrated roles and respect phagocytic territories during neuronal corpse removal in vivo Sci Adv, 2020-06-26;6(26):eaba3239. 2020-06-26 [PMID: 32637606] (IF, Mouse)
• A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):. 2019-11-02 [PMID: 31689560] (Cell Culture, Mouse)
• RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer Cancer Res., 2019-03-15;0(0):. 2019-03-15 [PMID: 30877108] (Flow Cytometry, Mouse)
• D Aran, AP Looney, L Liu, E Wu, V Fong, A Hsu, S Chak, RP Naikawadi, PJ Wolters, AR Abate, AJ Butte, M Bhattachar Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage Nat. Immunol., 2019-01-14;20(2):163-172. 2019-01-14 [PMID: 30643263] (IHC-Fr, Mouse)
• DG Thomas, AC Doran, P Fotakis, M Westerterp, P Antonson, H Jiang, XC Jiang, JÅ Gustafsson, I Tabas, AR Tall LXR Suppresses Inflammatory Gene Expression and Neutrophil Migration through cis-Repression and Cholesterol Efflux Cell Rep, 2018-12-26;25(13):3774-3785.e4. 2018-12-26 [PMID: 30590048] (Neutralization, Mouse)
• W Du, H Huang, N Sorrelle, RA Brekken Sitravatinib potentiates immune checkpoint blockade in refractory cancer models JCI Insight, 2018-11-02;3(21):. 2018-11-02 [PMID: 30385724] (Western Blot, Mouse)
• CEJ Waterborg, S Beermann, MGA Broeren, MB Bennink, MI Koenders, PLEM van Lent, WB van den Be, PM van der Kr, FAJ van de Loo Protective Role of the MER Tyrosine Kinase via Efferocytosis in Rheumatoid Arthritis Models Front Immunol, 2018-04-13;9(0):742. 2018-04-13 [PMID: 29706963] (Activation, Immunoprecipitation, In Vivo, Western Blot, Mouse)
• A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury Proc. Natl. Acad. Sci. U.S.A., 2017-09-05;0(0):. 2017-09-05 [PMID: 28874532] (IHC, Western Blot, Mouse)
• A Canfrán-Du, N Rotllan, X Zhang, M Fernández-, C Ramírez-Hi, E Araldi, L Daimiel, R Busto, C Fernández-, Y Suárez Macrophage deficiency of miR-21 promotes apoptosis, plaque necrosis, and vascular inflammation during atherogenesis EMBO Mol Med, 2017-09-01;0(0):. 2017-09-01 [PMID: 28674080] (Flow Cytometry, Western Blot, Mouse)
• M Bellesi, L de Vivo, M Chini, F Gilli, G Tononi, C Cirelli Sleep Loss Promotes Astrocytic Phagocytosis and Microglial Activation in Mouse Cerebral Cortex J. Neurosci., 2017-05-24;37(21):5263-5273. 2017-05-24 [PMID: 28539349] (IHC, Western Blot, Mouse)
• A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain Neuron, 2017-05-03;94(3):500-516.e9. 2017-05-03 [PMID: 28472653] (Neutralization, Mouse)
• M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa Proc. Natl. Acad. Sci. U.S.A, 2017-01-03;0(0):. 2017-01-03 [PMID: 28049839] (Western Blot, Mouse)
• MicroRNA-126 overexpression rescues diabetes-induced impairment in efferocytosis of apoptotic cardiomyocytes Sci Rep, 2016-11-09;6(0):36207. 2016-11-09 [PMID: 27827458] (Immunoprecipitation, Mouse)
• Liver X receptor and STAT1 cooperate downstream of Gas6/Mer to induce anti-inflammatory arginase 2 expression in macrophages Sci Rep, 2016-07-13;6(0):29673. 2016-07-13 [PMID: 27406916] (Mouse)
• MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation Proc Natl Acad Sci USA, 2016-05-19;0(0):. 2016-05-19 [PMID: 27199481] (Western Blot, Mouse)
• L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke TAM receptors regulate multiple features of microglial physiology Nature, 2016-04-06;532(7598):240-4. 2016-04-06 [PMID: 27049947] (Western Blot, Mouse)
• Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells. J Immunol, 2015-04-27;194(11):5200-10. 2015-04-27 [PMID: 25917096] (Flow Cytometry, Mouse)
• Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease. J Neurosci, 2015-04-22;35(16):6532-43. 2015-04-22 [PMID: 25904803] (Flow Cytometry, IHC, Western Blot, Mouse)
• Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med, 2015-02-01;7(2):211-26. 2015-02-01 [PMID: 25604058] (Western Blot, Mouse)
• Zagorska A, Traves P, Lew E, Dransfield I, Lemke G Diversification of TAM receptor tyrosine kinase function. Nat Immunol, 2014-09-07;15(10):920-8. 2014-09-07 [PMID: 25194421] (Immunoprecipitation, Neutralization, Mouse)
• Nascimento M, Huang S, Smith A, Everts B, Lam W, Bassity E, Gautier E, Randolph G, Pearce E Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis. PLoS Pathog, 2014-08-21;10(8):e1004282. 2014-08-21 [PMID: 25144366] (Flow Cytometry, Mouse)
• Richardson , Monica L, Fu , Chi-Ling, Pennington , Luke F, Honeycutt , Jared D, Odegaard , Justin I, Hsieh , Yi-Ju, Hammam , Olfat, Conti , Simon L, Hsieh , Michael A new mouse model for female genital schistosomiasis. PLoS Negl Trop Dis, 2014-05-01;8(5):e2825. 2014-05-01 [PMID: 24786606] (Flow Cytometry, Mouse)
• Jakubzick C, Gautier E, Gibbings S, Sojka D, Schlitzer A, Johnson T, Ivanov S, Duan Q, Bala S, Condon T, Van Rooijen N, Grainger J, Belkaid Y, Ma'ayan A, Riches D, Yokoyama W, Ginhoux F, Henson P, Randolph G Minimal differentiation of classical monocytes as they survey steady-state tissues and transport antigen to lymph nodes. Immunity, 2013-09-05;39(3):599-610. 2013-09-05 [PMID: 24012416] (Flow Cytometry, Mouse)
• Brown JE, Krodel M, Pazos M Cross-phosphorylation, signaling and proliferative functions of the Tyro3 and Axl receptors in Rat2 cells. PLoS ONE, 2012-05-14;7(5):e36800. 2012-05-14 [PMID: 22606290] (Immunoprecipitation, Rat)
• Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-kappaB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury. J. Cell. Sci., 2012-03-16;91(6):921-32. 2012-03-16 [PMID: 22427680] (ICC, Neutralization, Mouse)
• Galvan MD, Foreman DB, Zeng E Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells. J. Immunol., 2012-03-14;188(8):3716-23. 2012-03-14 [PMID: 22422887] (Western Blot, Mouse)
• Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J. Neurosci., 2010-11-17;30(46):15521-34. 2010-11-17 [PMID: 21084607] (ICC, Western Blot, Mouse)
• Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010-03-26;115(23):4963-72. 2010-03-26 [PMID: 20348395] (Western Blot, Mouse)
• Thorp E, Cui D, Schrijvers DM, Kuriakose G, Tabas I Mertk receptor mutation reduces efferocytosis efficiency and promotes apoptotic cell accumulation and plaque necrosis in atherosclerotic lesions of apoe-/- mice. Arterioscler. Thromb. Vasc. Biol., 2008-05-01;28(8):1421-8. 2008-05-01 [PMID: 18451332] (IHC-P, Mouse)
• Chang Y, Finnemann SC Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis. J. Cell. Sci., 2007-08-07;120(0):3053-63. 2007-08-07 [PMID: 17684062] (Immunoprecipitation, Rat)
• Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation. Blood, 2006-10-17;109(3):1026-33. 2006-10-17 [PMID: 17047157] (Flow Cytometry, Western Blot, Mouse)
• Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 2005-02-23;3(4):733-41. 2005-02-23 [PMID: 15733062] (Flow Cytometry, Mouse)

Reviews for Mer Antibody (AF591) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Fuyi Chen immunohistochemistry free floating section Mouse 03/16/2018

Summary

• Application: Other
• Sample Tested: Adult mouse brain
• Species: Mouse
• Lot: DGS031611B

Comments

• Comments: antigen retrieval for 15 mins. 1:250 dilution.

reviewed by: Verified Customer in vivo Mouse 04/03/2017

Summary

• Application: Other
• Sample Tested: Adult mouse brain,brain and spinal cord
• Species: Mouse
• Lot: DGS0416031

Comments

• Comments: Used in vivo to inhibit Mer activation.

Bioinformatics

• Gene Symbol: MERTK
• Uniprot:
  • Q60805()