Novus Biologicals products are now on bio-techne.com

Integrin beta 3/CD61 Antibody (SJ19-09)

Images

 
Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse lung tissue. IF-stained paraffin section. Image from verified customer review.
Western Blot: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Mouse bone marrow cell lysate with DMSO (lane 1), chemical agonist #1 (lane 2), chemical agonist #2 (lane 3), chemical agonist #3 (lane 4). WB image ...read more
Immunocytochemistry/ Immunofluorescence: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Staining Integrin beta 3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (SJ19-09) [NBP2-67416] - Analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SJ19-09
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Integrin beta 3/CD61 Antibody (SJ19-09) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human Integrin beta 3/CD61 aa 729-788 / 788. (SwissProt: P05106 Human; SwissProt: O54890 Mouse; Entrez Gene: 29302 Rat)
Localization
Cell membrane, Cell projection, Cell junction.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ITGB3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Western Blot 1:1000
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-67416 in the following applications:

Publications
Read Publications using
NBP2-67416 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Integrin beta 3/CD61 Antibody (SJ19-09)

  • CD61 antigen
  • CD61
  • GP3A
  • GP3Aplatelet glycoprotein IIIa
  • GPIIIA
  • GPIIIaintegrin beta-3
  • INGRB3
  • Integrin beta 3
  • integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
  • ITG B3
  • ITGB3
  • Platelet membrane glycoprotein IIIa

Background

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB7616
Species: Hu
Applications: CyTOF-ready, Flow, WB
NB110-68136
Species: Fe, Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-81858
Species: Hu
Applications: IHC, IHC-P, WB
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
137-PS
Species: Hu
Applications: BA
NBP1-97507
Species: Bv, Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-76544
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-88866
Species: Hu
Applications: IHC, IHC-P
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NB300-500
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
NBP1-89296
Species: Hu
Applications: IHC, IHC-P, WB
AF4117
Species: Rt
Applications: IHC, WB
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NB600-586
Species: Ca, Fe, Hu, Mu
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-232
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB

Publications for Integrin beta 3/CD61 Antibody (NBP2-67416)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.


Filter By Application
ICC/IF
(3)
IF/IHC
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
All Species

Reviews for Integrin beta 3/CD61 Antibody (NBP2-67416) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP2-67416:
Filter by Applications
WB
(1)
immunofluorescence staining
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Integrin beta 3/CD61 NBP2-67416
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 09/14/2019
View

Summary

ApplicationWestern Blot
Sample Testedmacrophage cell lysate,mouse primary cell
SpeciesMouse
LotHL1203
Other Integrin beta 3/CD61 NBP2-67416
Enlarge
5
reviewed by:
Verified Customer
immunofluorescence staining Mouse 07/05/2019
View

Summary

ApplicationOther
Sample TestedLung tissue
SpeciesMouse
LotHL1203

Comments

CommentsParaffin Section

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 3/CD61 Antibody (NBP2-67416) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Integrin beta 3/CD61 Products

Research Areas for Integrin beta 3/CD61 Antibody (NBP2-67416)

Find related products by research area.

Blogs on Integrin beta 3/CD61

There are no specific blogs for Integrin beta 3/CD61, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
09/14/2019
Application: WB
Species: Mouse

Verified Customer
07/05/2019
Application: immunofluorescence staining
Species: Mouse

Bioinformatics

Gene Symbol ITGB3