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Integrin alpha 8 Antibodies

We offer Integrin alpha 8 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Simple Western, Western Blot. Each Integrin alpha 8 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Integrin alpha 8 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Integrin alpha 8 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Integrin alpha 8 polyclonal antibodies and browse our Integrin alpha 8 monoclonal antibody catalog.

Alternate Names for Integrin alpha 8 Antibodies

anti-Integrin alpha 8 antibody, anti-ITGA8 antibody, anti-integrin alpha-8 antibody, anti-integrin, alpha 8 antibody

7 Results for "integrin-alpha-8" in Primary Antibodies

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AF4076

Integrin alpha 8 Antibody [Unconjugated]

(26 Publications)

100 μg

AF4076

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF4076

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4076-SP

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MAB6194

Integrin alpha 8 Antibody (481709) [Unconjugated]

(3 Publications)

100 μg

MAB6194

Integrin a8 was detected in immersion fixed H4 human neuroglioma cell line using Mouse Anti-Human Integrin a8 Monoclonal Antibody (Catalog # MAB6194) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6194U-100UG
Alexa Fluor 405	FAB6194V-100UG
Alexa Fluor 488	FAB6194G-100UG
Alexa Fluor 532	FAB6194X
Alexa Fluor 594	FAB6194T-100UG
Alexa Fluor 647	FAB6194R-100UG
Alexa Fluor 700	FAB6194N-100UG
Alexa Fluor 750	FAB6194S-100UG
Allophycocyanin	FAB6194A
Allophycocyanin/Cy7	FAB6194APCCY7
Biotin	FAB6194B
CoraFluor 1	FAB6194CL1
DyLight 350	FAB6194D
DyLight 405	FAB6194E
DyLight 488	FAB6194K
DyLight 550	FAB6194L
DyLight 594	FAB6194M
DyLight 650	FAB6194W
DyLight 680	FAB6194Y
DyLight 755	FAB6194Z
FITC	FAB6194F
HRP	FAB6194H
Janelia Fluor 525	FAB6194JF525
Janelia Fluor 549	FAB6194I
Janelia Fluor 646	FAB6194J
Janelia Fluor 669	FAB6194JF669
PE/Atto594	FAB6194PEATT594
PE/Cy5.5	FAB6194PECY55
PE/Cy7	FAB6194PECY7
PerCP	FAB6194C
Phycoerythrin	FAB6194P
mFluor Violet 450 SE	FAB6194MFV450
mFluor Violet 500 SE	FAB6194MFV500
mFluor Violet 610 SE	FAB6194MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB6194P
Carrier Free	FAB6194A
Carrier Free	MAB6194-SP
Carrier Free	FAB6194V-100UG
Carrier Free	FAB6194U-100UG
Carrier Free	FAB6194T-100UG
Carrier Free	FAB6194S-100UG
Carrier Free	FAB6194R-100UG
Carrier Free	FAB6194N-100UG
Carrier Free	FAB6194G-100UG

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NBP1-86519

Integrin alpha 8 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP1-86519

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP1-86519] - Staining of human lung shows weak to moderate membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody [NBP1-86519] - Staining of human small intestine shows weak to moderate membranous positivity in smooth muscle cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86519-25ul

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NBP2-76482

Integrin alpha 8 Antibody (CL7304) - BSA Free

100 ul

NBP2-76482

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Analysis in human lung and pancreas tissues. Corresponding ITGA8 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Staining of human lung shows strong membranous positivity in pneumocytes.

Immunohistochemistry-Paraffin: Integrin alpha 8 Antibody (CL7304) [NBP2-76482] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44412AF350
Alexa Fluor 405	NBP3-44412AF405
Alexa Fluor 488	NBP3-44412AF488
Alexa Fluor 532	NBP3-44412AF532
Alexa Fluor 594	NBP3-44412AF594
Alexa Fluor 647	NBP3-44412AF647
Alexa Fluor 700	NBP3-44412AF700
Alexa Fluor 750	NBP3-44412AF750
Allophycocyanin	NBP3-44412APC
Biotin	NBP3-44412B
CoraFluor 1	NBP3-44412CL1
DyLight 350	NBP3-44412UV
DyLight 405	NBP3-44412V
DyLight 488	NBP3-44412G
DyLight 550	NBP3-44412R
DyLight 594	NBP3-44412DL594
DyLight 650	NBP3-44412C
DyLight 680	NBP3-44412FR
DyLight 755	NBP3-44412IR
FITC	NBP3-44412F
HRP	NBP3-44412H
Janelia Fluor 525	NBP3-44412JF525
Janelia Fluor 549	NBP3-44412JF549
Janelia Fluor 646	NBP3-44412JF646
Janelia Fluor 669	NBP3-44412JF669
PE	NBP3-44412PE
PerCP	NBP3-44412PCP
mFluor Violet 450 SE	NBP3-44412MFV450
mFluor Violet 500 SE	NBP3-44412MFV500
mFluor Violet 610 SE	NBP3-44412MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44412
BSA Free	NBP2-76482-25ul

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H00008516-M02

Integrin alpha 8 Antibody (2G7) - Azide and BSA Free

(1 Publication)

0.1 mg

H00008516-M02

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-59940

Integrin alpha 8 Antibody - BSA Free

(1 Publication)

100 ul

NBP1-59940

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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BAF4076

Integrin alpha 8 Antibody [Biotin]

(7 Publications)

50 μg

BAF4076

Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse Integrin a8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of Mouse Lung and Rat Lung. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Integrin alpha 8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Integrin alpha 8 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Mesangial cells and not podocytes display considerable transient damage in the course of the EC model.Mice were analyzed at baseline (day 0), day 1, and day 7 following disease induction. A. Quantification of glomerular mesangial injury by alpha 8-integrin (mesangial cell marker) staining of kidney slices. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. **p<0.01, ***p<0.001 by one-way ANOVA; B. Representative alpha 8-integrin staining images in the course of the EC model. Scale bars correspond to 25 μm; C. Quantification of podocyte depletion and injury on kidney slices by WT1 and nephrin staining, respectively. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. *p<0.05, **p<0.01, ns- not significant by one-way ANOVA; D. Representative WT1/nephrin co-staining images in the course of the EC model. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

RLCs selectively differentiate to intraglomerular mesangial cells during EC model.A. Representative confocal microscopy images for day 0 and day 7 of beta -gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices; B. Representative confocal microscopy images for day 0 and day 7 of beta -gal and the mesangial cell markers PDGF beta R (upper panels) or alpha 8-integrin (lower panels) co-stained kidney slices; C. Representative confocal microscopy images for day 0 and day 7 of beta -gal/WT1 (podocyte marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker throughout. The channels for green ( beta -gal) and red (corresponding cell marker) fluorescent signals in the dashed squares on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Vascular development is limited in the reconstituted kidney organoids in vitro (A) Scheme for kidney organoid reconstitution and culture in vitro. Three types of kidney progenitors at E11.5 (NPs, SPs, and Hoxb7-GFP+ UBs) were aggregated overnight with or without ECs. The organoids were subsequently cultured at the air-liquid interface for 2–6 days (days 3–7). (B) Sorting of ECs, NP, and SPs. CD31+ and/or Flk1+ cells were sorted as ECs (left), and the cells in the CD31−/Flk1− fraction (left) were further sorted into Itga8+ NP and Pdgfra+ SP fractions (right). (C–F) Whole-mount staining of kidney organoids aggregated with ECs and cultured for the indicated days. GFP+ UB branching and CD31+ vasculature formation (C,D), as well as Nephrin+ glomerulus formation (E), were observed. No Cx40+ arterioles (F) were observed. (G) Section staining of glomeruli (nephrin). No CD31+ ECs were detected in glomeruli. (H–L) Whole-mount (H–K) or section (L) staining of kidney organoids aggregated without ECs and cultured for the indicated days. No apparent differences between the organoids with (C–G) and without (H–L) ECs were observed. Scale bars: 100 µm (C–F, H–K); 10 µm (G,L). Representative images of three organoids each with and without ECs from three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30718617), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Biotin

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF4076
Carrier Free	AF4076-SP

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Integrin alpha 8 Antibodies

Integrin alpha 8 Antibody [Unconjugated]

Integrin alpha 8 Antibody (481709) [Unconjugated]

Integrin alpha 8 Antibody - BSA Free

Integrin alpha 8 Antibody (CL7304) - BSA Free

Integrin alpha 8 Antibody (2G7) - Azide and BSA Free

Integrin alpha 8 Antibody - BSA Free

Integrin alpha 8 Antibody [Biotin]