FGFR1 Antibody (M19B2)

Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.

Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.

Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: M19B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FGFR1 Antibody (M19B2) Summary

• Immunogen: Recombinant human ectodomain of FGFR1b expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
• Epitope: Epitope is within the NH2-terminus of Ig loop II sequence lys145 and glu159 between the acidic box and heparin-binding sequence of FGFR1.
• Localization: Cell Membrane
• Specificity: Reacts with both alpha and beta isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Flow Cytometry 1 ug per million cells
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
• Application Notes: This antibody is CyTOF ready. Use in Immunohistochemistry reported in scientific literature (PMID: 25182532).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FGFR1 Antibody (M19B2)

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Background

FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1 Antibody (NB600-1287)(4)

Publications using NB600-1287

• Shih CH, Chiang TB, Wang WJ. Synergistic suppression of a disintegrin acurhagin-C in combination with AZD4547 and reparixin on terminating development for human osteosarcoma MG-63 cell Biochem. Biophys. Res. Commun. 2017 Aug 17 [PMID: 28823917] (Flow, Human)
• Schwenk BM, Hartmann H, Serdaroglu A et al. TDP-43 loss of function inhibits endosomal trafficking and alters trophic signaling in neurons EMBO J. Sep 12 2016 [PMID: 27621269] (WB, Human)
• Fu T, Seok S, Choi S et al. MiR-34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte FGF21 signaling and SIRT1 function. Mol Cell Biol. 2014 Sep 02 [PMID: 25182532]
• Sasaki S, Ishida T, Toyota M et al. Interferon-alpha/beta and anti-fibroblast growth factor receptor 1 monoclonal antibody suppress hepatic cancer cells in vitro and in vivo. PLoS One. 2011 May 9 [PMID: 21573021] (FLOW, Human)

Review for FGFR1 Antibody (NB600-1287) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Lisa Renshaw Flow Other 12/16/2010

Summary

• Application: Flow Cytometry
• Sample Tested: transfected cell line
• Species: Other
• Comments: we get great FACS results with Novus antibodies

Comments

• Comments: we get great FACS results with Novus antibodies

FAQs for FGFR1 Antibody (NB600-1287). (Showing 1 - 2 of 2 FAQs).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Bioinformatics

• Gene Symbol: FGFR1
• Entrez:
  • 2260(Human)
  • 79114(Rat)
• Uniprot:
  • P11362()
  • Q04589(Rat)