Insulin Antibody (8E2)

Product Discontinued

Insulin Antibody (8E2) Summary

• Immunogen: Derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
• Specificity: This cross-reacts with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture-detection): NB100-73008 - 8E2

Reactivity Notes

Insulin, human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Insulin Antibody (8E2)

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipi d metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Insulin Antibody (NB100-73009)(13)

Publications using NB100-73009

• Bima A, Eldakhakhny B, Alamoudi AA et al. Molecular Study of the Protective Effect of a Low-Carbohydrate, High-Fat Diet against Brain Insulin Resistance in an Animal Model of Metabolic Syndrome Brain sciences 2023-09-28 [PMID: 37891752] (ELISA, Rat)
• Matilla L, Martín-Núñez E, Garaikoetxea M et al. Sex-specific role of galectin-3 in aortic stenosis Biology of sex differences 2023-10-24 [PMID: 37875993]
• Yang C, Zhu X, Liu W et al. Dietary Dried Jujube Fruit Powder (DJFP) Supplementation Improves Growth Performance, Antioxidant Stability, and Meat Composition in Broilers Foods (Basel, Switzerland) 2023-03-29 [PMID: 37048283] (ELISA, Chicken)
• Kannan A, Beal JR, Neff AM et al. Runx1 regulates critical factors that control uterine angiogenesis and trophoblast differentiation during placental development bioRxiv : the preprint server for biology 2023-03-23 [PMID: 36993295] (IHC-P, Mouse)
  
  Details:
  Dilutions: 1:200
• Stout MB, Vaughan KL, Isola JVV et al. Assessing tolerability and physiological responses to 17?-estradiol administration in male rhesus macaques GeroScience 2023-03-10 [PMID: 36897526]
• Zhou X, Zhang H, Jiang Y et al. The role of chemerin in the regulation of cGAS-STING pathway in gestational diabetes mellitus placenta FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023-03-01 [PMID: 36786722] (ELISA, Human)
• Iker Etchegaray J, Kelley S, Penberthy K et al. Phagocytosis in the retina promotes local insulin production in the eye Nature metabolism 2023-02-01 [PMID: 36732622]
• Deng Z, Kuno A, Ojima M, Takahashi S MafB Maintains beta-Cell Identity under MafA-Deficient Conditions Molecular and cellular biology 2022-07-11 [PMID: 35862726]
• Chong A, Vandana J, Li G Et Al. Checkpoint Kinase 2 Controls Insulin Secretion and Glucose Homeostasis SSRN Electronic Journal 2021-07-23 (ELISA)
• Chow CFW, Guo X, Asthana P et al. Body weight regulation via MT1-MMP-mediated cleavage of GFRAL Nature metabolism 2022-02-01 [PMID: 35177851]
• Abd El Kader M, Gabr M, Khater S Et al. Impact of Insulin Producing Cells Derived from Adipose Tissue Mesenchymal Stem Cells on Testicular Dysfunction of Diabetic Rats Heliyon 2021-11-01 [PMID: 34820536] (ICC/IF, Rat)
• Srivastava N, Hu H, Vomund AN et al. Chromogranin A Deficiency Confers Protection From Autoimmune Diabetes via Multiple Mechanisms Diabetes 2021-09-08 [PMID: 34497137]
  
  Details:
  Citation using the HRP format of this antibody.
• Woo VS Western Diet and Early Life Stress Program Insulin Secretion in Adulthood J Endocrinol 2013-10-08 [PMID: 24096964]

FAQs for Insulin Antibody (NB100-73009). (Showing 1 - 1 of 1 FAQs).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Bioinformatics

• Gene Symbol: INS
• Entrez:
  • 3630(Human)