Insulin Antibody (3A6)

Product Discontinued

Insulin Antibody (3A6) Summary

• Immunogen: Derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
• Specificity: This cross-reacts with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA 1:100-1:2000
• Application Notes: Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture-detection): NB100-73008 - href= "/products/Insulin-Antibody-8E2_NB100-73009" >NB100-73009. Use in ELISA reported in scientific literature (PMID 28132934).

Reactivity Notes

Insulin, human. Mouse reactivity reported in scientific literature (PMID: 28132934).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Insulin Antibody (3A6)

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Insulin Antibody (NB100-73008)(5)

Publications using NB100-73008

• Lopez-MuNoz G, FernAndez-Costa J, Ortega M Et al. Plasmonic nanocrystals on polycarbonate substrates for direct and label-free biodetection of Interleukin-6 in bioengineered 3D skeletal muscles Nanophotonics 2021-10-08
• Lin X, Wang G, Ma L, Liu G Study on Factors Affecting the Performance of a CRISPR/Cas-Assisted New Immunoassay: Detection of Salivary Insulin as an Example Frontiers in Bioengineering and Biotechnology 2021-11-11 [PMID: 34858958]
• Ortega MA, RodrIguez-Comas J, Yavas O et al. In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip Biosensors 2021-04-28 [PMID: 33924867] (ELISA, Mouse)
• Cao Y, Kakar P, Hossen MN et al. Sustained epidermal powder drug delivery via skin microchannels J Control Release 2017-01-27 [PMID: 28132934] (ELISA, Mouse)
• Fernandez Costa J, Ortega M, RodrIguez Comas J et al. Training on a Chip: A Multi Organ Device to Study the Effect of Muscle Exercise on Insulin Secretion in Vitro Advanced Materials Technologies 2022-08-23 (Human)
  
  Details:
  Microfluidics

FAQs for Insulin Antibody (NB100-73008). (Showing 1 - 2 of 2 FAQs).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.
2. 1) What is the available longest shelf life you currently have in stock? And best recommended storage temperature? 2) Could please recommend another antibody which may pair well with NB100-73008 in Sandwich ELISA? Could you please recommend the type of plate (eg. 96 or 480 well plates, and whether strip or solid) that would be best suited to carrying to the Sandwich ELISA using the above provided antibody and another antibody pair which you may recommend?
   • We recommend storing this antibody at 4 degrees C and do not recommend freezing. This antibody, along with all others, we guarantee for 6 months from date of receipt. If the customer would like to use NB100-73008 as the capture antibody, we recommend NBP1-50246 as the detection antibody. As far as what sort of plate for the customer to use, traditionally, ELISA is done in a 96-well plate, but any medium that the customer would prefer should work just fine.

Bioinformatics

• Gene Symbol: INS
• Entrez:
  • 3630(Human)