Insulin Antibody (2D11-H5) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P, CyTOF-ready
Clone
2D11-H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Insulin Antibody (2D11-H5) - Azide and BSA Free Summary

Immunogen
The antibody reacts with the Insulin antigen.
Specificity
Insulin is a protein consisting of an alpha-chain of 21 amino acids and a beta-chain of 30 amino acids and produced in the beta-cells of the pancreas. 2D11-H5 is a specific insulin mAb as tested by ELISA and on human pancreatic tissue. Source: A BALB/c mouse was immunised with swine insulin. The mouse spleen cells were fused with SP2/0 Ag14 cells and the clones were selected on anti-insulin activity.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
INS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • CyTOF-ready
Application Notes
Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen and Immunoprecipitation applications cited in literature.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Insulin Antibody (2D11-H5) - Azide and BSA Free

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Insulin Antibody (NBP2-33146) (0)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Insulin Antibody (NBP2-33146). (Showing 1 - 1 of 1 FAQ).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Insulin Antibody (NBP2-33146)

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Blogs on Insulin.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.

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Signalling Advances in Adiponectin Antibody Research
Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I...  Read full blog post.

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Bioinformatics

Gene Symbol INS
Entrez